ETHNIC PATTERNS OF ARTERIAL PRESSURE DAILY PROFILE, AUTONOMIC REGULATION OF CARDIAC FUNCTION AND METABOLIC SYSTEM IN PREGNANT WOMEN WITH ARTERIAL HYPERTENSION

Arterial hypertension (AH) is observed in 4-8 % of pregnant women. Even light previous AH increases the risk of pregnancy and delivery complications up to twice, thereby significantly degrading the prognosis of pregnancy and childbirth. The aim of the study is to evaluate the main cardiovascular and metabolic patterns in pregnant women with AH in depends on their ethnicity. We studied the pregnancy courses in 295 pregnant women with AH. The 1st group was 138 Buryat women and the 2nd group were Russian. In all patients we evaluated hematological, biochemical and hemostatic data as soon as perform daily monitoring of blood pressure. We observed some ethnic patterns of arterial hypertension. Buryat women had dyslipidemia and elevated fibrinogen. Also these patients demonstrated elevation of the value of morning rise in diastolic blood pressure during (DBP) their pregnancies with the most significant value of variability of night DBP. We found the direct link of serum fibrinogen with night DBP в In the 2nd trimester of pregnancy

DISCRIMINANT ANALYSIS AS A METHOD OF DETERMINATION OF GESTATIONAL PERIOD IN DRINKING WOMEN AND HEALTH CONDITION OF THEIR NEWBORNS

The article presents the methods of mathematical analysis of the impactof alcohol on pregnancy, childbirth, postpartum and the state of health of newborn state. With the help of this analysis we revealed informative diagnostic features that let determine the effect of alcohol on pregnancy, childbirth, postpartum and newborn state: the use of alcohol before pregnancy once per month; alcohol consumption before pregnancy 2-4 times per month; smoking during pregnancy; abortion history; anemia during pregnancy; congenital heart disease on the third ultrasound screening; abnormal birth activity; subinvolution of uterus in the postpartum period; newborn gender; gestational age; prematurity and generally heavy state of a newborn. Linear classification function can be calculated by the formula 1.2. Canonical linear discriminant function can be calculated from equation [formula 3]. Diagnostic accuracy of decision rules in the middle is quite high - 76,69 %, in the first group - 75,7 %, in the second group - 77,6 %

Lipid peroxidation and antioxidant protection systems in pregnant women consuming alcohol in small and moderate doses

The analysis of scientific researches shows that the problem of alcohol consumption (beer, dry wine, champagne), in small doses during pregnancy has been actually disregarded for many years. So the problem has become quite urgent, especially in Russia. In order to determine the effect of alcohol on pregnancy and childbirth, women and babies were divided into three groups. Group 1 (n = 101) (control group) included women who did not use alcohol during pregnancy. Group 2 (n = 75) included drinking women whose alcohol consumption during pregnancy was less than 2 doses or 750 ml. Group 3 (n = 33) included moderately drinking women whose alcohol consumption during pregnancy ranged from 3 to 11 doses (from 750 ml to 3850 ml). The study of the lipid peroxidation-antioxidant protection system as a sensitive marker of integrated health disorders in women, consuming alcoholic beverages in a prenatal period, was conducted. It was indicated that a statistically significant decrease in overall antioxidant activity was found in the context of lipid peroxidation activation in women consuming low-alcohol drinks, which demonstrates a lack of functioning of lipid peroxidation system and oxidative stress regardless of the dose of low-alcohol drinks

Influence of factors of solar and geomagnetic activity on development of spontaneous 82 labor activity

Studies of recent decades have proved the effect on the human body a number of physical factors that are caused by the influence of solar corpuscular streams that change the state of the magnetosphere. The least studied problem is the influence of heliogeophysical factors and solar activity on different periods of organism development, from conception to birth. There is no data on the influence of magnetic storms on spontaneous labor, which was the purpose of this study. Solar activity indicators are presented by the Space Weather Prediction Center (SWPC) of the US National Oceanic and Atmospheric Administration (NOAA: www.swpc.noaa.gov). The results of correlation analysis between spontaneous labor activity of 306 pregnant women in the period of 1.01-08.02.2013 depending on solar activity (Wolf numbers), radio emission flux at the wavelength of 10.7 cm and planetary index (Ap-index) characterizing the physical state of the Earth's magneticfield are analyzed. Reliable correlation between the number of urgent delivery and the flow of radio emission at a wavelength of 10.7 cm and the Wolf numbers was not found. Increase in the activity of spontaneous labor by 2.3 times (r = 0.43; p < 0.05) was recorded three days after the magnetic storm (Ap-index = 21nTl) compared to days calm in geomagnetic terms. The data showed a significant role of dynamics of external factors in the onset of labor

The influence of the heliogeophysical factors on the clotting of blood of pregnant women with placental insufficiency

Geomagnetic storms are one of the natural abiotic risk factors for human health. The response of the organism to their impact depends on the individual adaptive abilities, which were formed during the evolution, but can change in the conditions of pathological processes. Evaluation of the effect of a weak magnetic storm (G4v (22 < Ar < 39 nTl) on hemorheological parameters of blood of pregnant women with placental insufficiency compared to healthy pregnant women showed an increase in platelet count (p = 0.05), fibrinogen content (p = 0.02), thrombocyte (p = 0.03), erythrocyte sedimentation rate (p = 0.004). At the same time, a decrease in the number of red blood cells (p = 0.04), hemoglobin concentration (p = 0.001), hematocrit value (p = 0.0009) was registered. We found a strong direct correlation between subpopulations of platelets, which are characterized by different functional activity. Heterogeneity of platelets, their multidirectional correlations with indicators of blood coagulation system of healthy pregnant women can be considered as a response to the change in the disturbance of the earth's magnetic field. For pregnant women with placental disorders, the magnetic storm was associated with increased activity of the blood coagulation system and a significant increase in its components in the blood of patients. Despite the fact that changes in the blood coagulation system occur from the beginning of pregnancy and are often adaptive, in pregnant women with placental disorders, the additional impact of a weak magnetic storm can be a risk of hemostasis disorders and the development of platelet thrombosis, timely diagnosis and therapy of which is important

p53 Amino-Terminus Region (1–125) Stabilizes and Restores Heat Denatured p53 Wild Phenotype

BACKGROUND:The intrinsically disordered N-ter domain (NTD) of p53 encompasses approximately hundred amino acids that contain a transactivation domain (1-73) and a proline-rich domain (64-92) and is responsible for transactivation function and apoptosis. It also possesses an auto-inhibitory function as its removal results in remarkable reduction in dissociation of p53 from DNA. PRINCIPAL FINDINGS/METHODOLOGY:In this report, we have discovered that p53-NTD spanning amino acid residues 1-125 (NTD125) interacted with WT p53 and stabilized its wild type conformation under physiological and elevated temperatures, both in vitro and in cellular systems. NTD125 prevented irreversible thermal aggregation of heat denatured p53, enhanced p21-5'-DBS binding and further restored DBS binding activity of heat-denatured p53, in vitro, in a dose-dependent manner. In vivo ELISA and immunoprecipitation analysis of NTD125-transfected cells revealed that NTD125 shifted equilibrium from p53 mutant to wild type under heat stress conditions. Further, NTD125 initiated nuclear translocation of cytoplasmic p53 in transcriptionally active state in order to activate p53 downstream genes such as p21, Bax, PUMA, Noxa and SUMO. CONCLUSION/SIGNIFICANCE:Here, we showed that a novel chaperone-like activity resides in p53-N-ter region. This study might have significance in understanding the role of p53-NTD in p53 stabilization, conformational activation and apoptosis under heat-stress conditions

Nuclear and Chloroplast Microsatellites Show Multiple Introductions in the Worldwide Invasion History of Common Ragweed, Ambrosia artemisiifolia

BACKGROUND: Ambrosia artemisiifolia is a North American native that has become one of the most problematic invasive plants in Europe and Asia. We studied its worldwide population genetic structure, using both nuclear and chloroplast microsatellite markers and an unprecedented large population sampling. Our goals were (i) to identify the sources of the invasive populations; (ii) to assess whether all invasive populations were founded by multiple introductions, as previously found in France; (iii) to examine how the introductions have affected the amount and structure of genetic variation in Europe; (iv) to document how the colonization of Europe proceeded; (v) to check whether populations exhibit significant heterozygote deficiencies, as previously observed. PRINCIPAL FINDINGS: We found evidence for multiple introductions of A. artemisiifolia, within regions but also within populations in most parts of its invasive range, leading to high levels of diversity. In Europe, introductions probably stem from two different regions of the native area: populations established in Central Europe appear to have originated from eastern North America, and Eastern European populations from more western North America. This may result from differential commercial exchanges between these geographic regions. Our results indicate that the expansion in Europe mostly occurred through long-distance dispersal, explaining the absence of isolation by distance and the weak influence of geography on the genetic structure in this area in contrast to the native range. Last, we detected significant heterozygote deficiencies in most populations. This may be explained by partial selfing, biparental inbreeding and/or a Wahlund effect and further investigation is warranted. CONCLUSIONS: This insight into the sources and pathways of common ragweed expansion may help to better understand its invasion success and provides baseline data for future studies on the evolutionary processes involved during range expansion in novel environments

New approaches in the diagnosis and treatment of latent tuberculosis infection

With nearly 9 million new active disease cases and 2 million deaths occurring worldwide every year, tuberculosis continues to remain a major public health problem. Exposure to Mycobacterium tuberculosis leads to active disease in only ~10% people. An effective immune response in remaining individuals stops M. tuberculosis multiplication. However, the pathogen is completely eradicated in ~10% people while others only succeed in containment of infection as some bacilli escape killing and remain in non-replicating (dormant) state (latent tuberculosis infection) in old lesions. The dormant bacilli can resuscitate and cause active disease if a disruption of immune response occurs. Nearly one-third of world population is latently infected with M. tuberculosis and 5%-10% of infected individuals will develop active disease during their life time. However, the risk of developing active disease is greatly increased (5%-15% every year and ~50% over lifetime) by human immunodeficiency virus-coinfection. While active transmission is a significant contributor of active disease cases in high tuberculosis burden countries, most active disease cases in low tuberculosis incidence countries arise from this pool of latently infected individuals. A positive tuberculin skin test or a more recent and specific interferon-gamma release assay in a person without overt signs of active disease indicates latent tuberculosis infection. Two commercial interferon-gamma release assays, QFT-G-IT and T-SPOT.TB have been developed. The standard treatment for latent tuberculosis infection is daily therapy with isoniazid for nine months. Other options include therapy with rifampicin for 4 months or isoniazid + rifampicin for 3 months or rifampicin + pyrazinamide for 2 months or isoniazid + rifapentine for 3 months. Identification of latently infected individuals and their treatment has lowered tuberculosis incidence in rich, advanced countries. Similar approaches also hold great promise for other countries with low-intermediate rates of tuberculosis incidence