Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea Deltoidea, Tribulus Terrestris and Panax Japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species Dioscorea deltoidea, Tribulus terrestris and Panax japonicus to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1–2.5 h (D. deltoidea and T. terrestris) or 1 h (P. japonicus) after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of D. deltoidea and T. terrestris acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39– 57%), blood-glucose level (by 46–51%), blood content in urine (by 78–80%) and total cholesterol (25– 36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in D. deltoidea; furostanol-type steroidal glycosides and quinic acid derivatives in T. terrestris; and ginsenosides and malonyl-ginsenosides in P. japonicus. These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This.Funding: Bioreactor cultivation of plant cell suspensions and their biochemical analysis were financially supported by the Ministry of Science and Higher Education of Russian Federation through Megagrant project no. 075-15-2019-1882 and performed by using the equipment of the large-scale research facilities “Experimental biotechnological facility” and “All-Russian Collection of cell cultures of higher plants” of the IPPRAS (EBF IPPRAS and ARCCC HP IPPRAS). Hypoglycemic activity evaluation of the cell biomass was performed with the financial support of the Russian Science Foundation project no. 19-14-00387. The results of the hypoglycemic activity evaluation were obtained by using the equipment of the Center for Collective Use “Analytical Center of the Federal State Budgetary Educational Institution of Higher Education Saint Petersburg State Chemical Pharmaceutical University (SPCPU) of the Ministry of Health of Russia”, equipped with the financial support of the Ministry of Education and Science of Russia

PROSPECTS OF USING PHYTOTHERAPY AT DISORDERS OF FAT AND CARBOHYDRATE METABOLISM

The review describes the first stage of phyto-pharmacological screening. It includes literature data analysis on promising species of medicinal plant raw materials, total extracts and individual substances of plant origin with pronounced pharmacological activity in disorders of fat and carbohydrate metabolism, incl. obesity, diabetes mellitus type 2, metabolic syndrome, and non-alcoholic fatty liver disease, confirmed in preclinical and/or clinical trials

EMBININ IS A PERSPECTIVE CARDIOTONIC MEAN FOR NATURAL ORIGIN

Embinin is a flavone C-glycoside from the aerial part of the milky white tangent (Iris lactea). Cardiotonic action was established earlier for the extract of Iris lactea. Identification of the active substance responsible for this type of biological activity seems to be an urgent task. The purpose of this study was to evaluate the cardiotropic effect and the toxicological profile of embinin. The acute toxicity of embinin was determined in accordance with GOST 32644-2014, the inotropic effect of embinin was evaluated in comparison with digoxin in the model of perfusion of the isolated rat heart by Langendorf, and also in vivo by echocardiographic study with calculation of the left ventricular shortening fraction and electrocardiogram analysis. The embinin in a dose of 2000 mg/kg didn’t cause the death of female rats. The substance has a pronounced inotropic effect. Establishment of a pronounced cardiotonic effect in the experiment and low toxicity, as well as a high probability of the presence of cardioprotective action of embinin, predicted using the PASS program, allow us to consider embinin as a promising molecule for further studies in cardiovascular disease

Profiling of Seed Proteome in Pea (<i>Pisum sativum</i> L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi

Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms&#8212;rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea (Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479