New cytotoxic benzo(b)thiophenilsulfonamide 1,1-dioxide derivatives inhibit a NADH oxidase located in plasma membranes of tumour cells

A series of benzo(b)thiophenesulfonamide 1,1-dioxide derivatives (BTS) have been designed and synthesized as candidate antineoplastic drugs. Several of these compounds have shown in vitro cytotoxic activity on leukaemic CCRF-CEM cells. The cytotoxic BTS, but not the inactive ones, were able to inhibit a tumour cell-specific NADH oxidase activity present in the membrane of CCRF-CEM cells. © 2001 Cancer Research Campaig

Protein engineering techniques and objectives

Grâce à l’utilisation de la mutagénèse dirigée, de la fusion cellulaire, de la fusion de gènes ou des modifications chimiques, il est maintenant possible d’obtenir des protéines de seconde génération. Ceci nous permet d’améliorer leur fonction, leur affinité pour le substrat ou le récepteur, leur spécificité, leur demi-vie, etc., l’un des principaux problèmes et le besoin le plus urgent, résideront dans la modification de leur immunogénicité.Through the use of site directed mutagenesis, cell or gene fusion, che mical modification, it is now possible to have access to second generation proteins. This allow us to improve their function, their affinity, their half life, etc. One of the main concern and urgent need will also be to modify their immunogenicity

Méthode originale de mesure de la radio activité des aliments d'origine animale

Morre Jean. Méthode originale de mesure de la radio-activité des aliments d’origine animale. In: Bulletin de l'Académie Vétérinaire de France tome 112 n°6, 1959. pp. 357-364

Combination of PCR targeting the VD2 of omp1 and reverse line blot analysis for typing of urogenital Chlamydia trachomatis serovars in cervical scrape specimens.

50% contained both serovars D and E. The nested VD2 PCR-RLB developed is a simple, fast, and specific method for the identification of individual urogenital C. trachomatis serovars previously detected by using plasmid PCR. Moreover, it is an appropriate method for studying multiple C. trachomatis infections and for use in large epidemiological studies

Sodium et potassium dans les aliments de régime et dans ceux du nourrisson

L’étude que nous avons faite et qui est loin d’être exhaustive, montre : — qu’il y a plusieurs sortes d’aliments de régime désodés avec des taux très différents : de 60 mg à 300 mg pour 100 g de produit frais ; — que le jambon cuit n’est pas, « priori, un aliment de régime désodé, seuls certains le sont. La saveur n’est pas un critère : il existe des jambons fades (aux polyphosphates) riches en sodium ; — que dans un régime le jaune de l’œuf doit être retenu pour 48 mg/100 g et le blanc pour 170 mg/100 g dans le calcul de l’apport de sodium ; — que la teneur en sodium est faible pour les fromages blancs, plus élevée pour les yaourts et très importante pour les fromages fondus ; — que du fait que l’alimentation du jeune bébé doit comporter peu de sodium, les aliments tout préparés doivent paraître fades à la mère ; celle-ci doit préférer les préparations aux fruits pour le nourrisson, car le taux de sodium est très faible : 8,4 mg/100 g contre 254 mg/100 g pour celles aux légumes ou aux viandes

Decomposition Analyses Applied to a Complex Ultradian Biorhythm: The Oscillating NADH Oxidase Activity of Plasma Membranes Having a Potential Time-Keeping (Clock) Function

Seasonal decomposition analyses were applied to the statistical evaluation of an oscillating activity for a plasma membrane NADH oxidase activity with a temperature compensated period of 24 min. The decomposition fits were used to validate the cyclic oscillatory pattern. Three measured values, average percentage error (MAPE), a measure of the periodic oscillation, mean average deviation (MAD), a measure of the absolute average deviations from the fitted values, and mean standard deviation (MSD), the measure of standard deviation from the fitted values plus R-squared and the Henriksson-Merton p value were used to evaluate accuracy. Decomposition was carried out by fitting a trend line to the data, then detrending the data if necessary, by subtracting the trend component. The data, with or without detrending, were then smoothed by subtracting a centered moving average of length equal to the period length determined by Fourier analysis. Finally, the time series were decomposed into cyclic and error components. The findings not only validate the periodic nature of the major oscillations but suggest, as well, that the minor intervening fluctuations also recur within each period with a reproducible pattern of recurrence. biological clockdecomposition analysesultradian rhythmtemperature compensationcircadian rhythmcultured cells

Prosthetic heart valve evaluation by magnetic resonance imaging

Objective: To evaluate the potential of magnetic resonance imaging (MRI) for evaluation of velocity fields downstream of prosthetic aortic valves. Furthermore, to provide comparative data from bileaflet aortic valve prostheses in vitro and in patients. Methods: A pulsatile flow loop was set up in a 7.0 Tesla MRI scanner to study fluid velocity data downstream of a 25 mm aortic bileaflet heart valve prosthesis. Three dimensional surface plots of velocity fields were displayed. In six NYHA class I patients blood velocity profiles were studied downstream of their St. Jude Medical aortic valves using a 1.5 Tesla MRI whole-body scanner. Blood velocity data were displayed as mentioned above. Results: Fluid velocity profiles obtained from in vitro studies 0.25 valve diameter downstream of the valve exhibited significant details about the cross sectional distribution of fluid velocities. This distribution completely reflected the valve design. Blood velocity profiles in humans were considerably smoother and in some cases skewed with the highest velocities toward the anterior-right ascending aortic wall. Conclusion: Display and interpretation of fluid and blood velocity data obtained downstream of prosthetic valves is feasible both in vitro and in vivo using the MRI technique. An in vitro model with a straight tube and the test valve oriented orthogonally to the long axis of the test tube does not entail fluid velocity profiles which are compatible to those obtained from humans, probably due to the much more complex human geometry, and variable alignment of the valve with the ascending aorta. With the steadily improving quality of MRI scanners this technique has significant potential for comparative in vitro and in vivo hemodynamic evaluation of heart valve