3 research outputs found
Dansyl Derivatives of Cholic Acid as Tools to Build Speciation Diagrams for Sodium Cholate Aggregation
Three fluorescent cholic acid derivatives incorporating the dansyl chromophore at positions 3α, 3β, and 7α have been successfully employed to build the speciation diagram of sodium cholate by means of steady-state and time-resolved fluorescence
Dansyl-Labeled Cholic Acid as a Tool To Build Speciation Diagrams for the Aggregation of Bile Acids
Bile acids (BAs) are a family of natural steroids biosynthesized
from cholesterol in the liver that tend to form aggregates in solution.
A fluorescent derivative of cholic acid, namely 3α-Dns-ChA,
was employed as a reporter to establish the speciation diagrams of
the most abundant BAs that can be found mainly in three microenvironments,
solution, and primary and secondary aggregates. The developed methodology
is based on the analysis of the combined steady-state and time-resolved
experiments performed on 3α-Dns-ChA whose emission behavior
was found to be strongly medium dependent. In particular, speciation
diagrams of sodium glycocholate (NaGCh), sodium taurocholate (NaTCh),
sodium chenodeoxycholate (NaCDCh), sodium glycochenodeoxycholate (NaGCDCh),
sodium deoxycholate (NaDCh), and sodium ursodeoxycholate (NaUDCh)
were successfully built up
Photophysical Probes To Assess the Potential of Cholic Acid Aggregates as Drug Carriers
The two enantiomers of the nonsteroidal antiinflammatory
drug naproxen
and of its methyl ester have been selected as representative probes
with markedly different hydrophobicity to assess the potential of
cholic acid aggregates as drug carriers by means of photophysical
techniques. The different distribution of the probes between bulk
solution and aggregates has been assessed by quenching of their singlet
and triplet excited states by iodide and nitrite anions, respectively.
This straightforward photophysical methodology can, in principle,
be extended to a variety of drugs containing a photoactive chromophore