Role of new theatrical practices in forming of cultural environment

На основе социологических опросов актеров и зрителей театра рассматриваются проблемы развития культурной среды региона.On the basis of the sociological questioning of actors and audience of theater the problems of development of cultural environment of region are examined

Exactly solvable model of wormhole supported by phantom energy

We have found a simple exact solution of spherically-symmetrical Einstein equations describing a wormhole for an inhomogeneous distribution of the phantom energy. The equation of state is linear but highly anisotropic: while the radial pressure is negative, the transversal one is positive. At infinity the spacetime is not asymptotically flat and possesses on each side of the bridge a regular cosmological Killing horizon with an infinite area, impenetrable for any particles. This horizon does not arise if the wormhole region is glued to the Schwarzschild region. In doing so, the wormhole can enclose an arbitrary amount of the phantom energy. The configuration under discussion has a limit in which the phantom energy turns into the string dust, the areal radius tends to the constant. In this limit, the strong gravitational mass defect is realized in that the gravitational active mass is finite and constant while the proper mass integrated over the total manifold is infinite.Comment: 6 pages. Two references added, typos corrected. Accepted for publication in Phys. Rev. D as Rapid Communicatio

Generalized Swiss-cheese cosmologies: Mass scales

We generalize the Swiss-cheese cosmologies so as to include nonzero linear momenta of the associated boundary surfaces. The evolution of mass scales in these generalized cosmologies is studied for a variety of models for the background without having to specify any details within the local inhomogeneities. We find that the final effective gravitational mass and size of the evolving inhomogeneities depends on their linear momenta but these properties are essentially unaffected by the details of the background model.Comment: 10 pages, 14 figures, 1 table, revtex4, Published form (with minor corrections

The standard "static" spherically symmetric ansatz with perfect fluid source revisited

Considering the standard "static" spherically symmetric ansatz ds2 = -B(r) dt2 + A(r) dr2 + r2 dOmega2 for Einstein's Equations with perfect fluid source, we ask how we can interpret solutions where A(r) and B(r) are not positive, as they must be for the static matter source interpretation to be valid. Noting that the requirement of Lorentzian signature implies A(r) B(r) >0, we find two possible interpretations: (i) The nonzero component of the source four-velocity does not have to be u0. This provides a connection from the above ansatz to the Kantowski-Sachs (KS) spacetimes. (ii) Regions with negative A(r) and B(r) of "static" solutions in the literature must be interpreted as corresponding to tachyonic source. The combinations of source type and four-velocity direction result in four possible cases. One is the standard case, one is identical to the KS case, and two are tachyonic. The dynamic tachyonic case was anticipated in the literature, but the static tachyonic case seems to be new. We derive Oppenheimer-Volkoff-like equations for each case, and find some simple solutions. We conclude that new "simple" black hole solutions of the above form, supported by a perfect fluid, do not exist.Comment: 24 standard LaTeX pages, no figures. Some change in emphasis; changes in parametrizations of some of the solutions (ND2, TD2, TD3, NS1); one new solution (TS4); removal of an incorrect statement (about ND4

Identification of RIP1 kinase as a specific cellular target of necrostatins

Necroptosis is a cellular mechanism of necrotic cell death induced by apoptotic stimuli in the form of death domain receptor engagement by their respective ligands under conditions where apoptotic execution is prevented. Although it occurs under regulated conditions, necroptotic cell death is characterized by the same morphological features as unregulated necrotic death. Here we report that necrostatin-1, a previously identified small-molecule inhibitor of necroptosis, is a selective allosteric inhibitor of the death domain receptor–associated adaptor kinase RIP1 in vitro. We show that RIP1 is the primary cellular target responsible for the antinecroptosis activity of necrostatin-1. In addition, we show that two other necrostatins, necrostatin-3 and necrostatin-5, also target the RIP1 kinase step in the necroptosis pathway, but through mechanisms distinct from that of necrostatin-1. Overall, our data establish necrostatins as the first-in-class inhibitors of RIP1 kinase, the key upstream kinase involved in the activation of necroptosis

Static charged perfect fluid spheres in general relativity

Interior perfect fluid solutions for the Reissner-Nordstrom metric are studied on the basis of a new classification scheme. General formulas are found in many cases. Explicit new global solutions are given as illustrations. Known solutions are briefly reviewed.Comment: 23 pages, Revtex (galley), journal version, to appear in Phys.Rev.

Plant-Derived Polysaccharide Supplements Inhibit Dextran Sulfate Sodium-Induced Colitis in the Rat

Several plant-derived polysaccharides have been shown to have anti-inflammatory activity in animal models. Ambrotose complex and Advanced Ambrotose are dietary supplements that include aloe vera gel, arabinogalactan, fucoidan, and rice starch, all of which have shown such activity. This study was designed to evaluate these formulations against dextran sulfate sodium (DSS)-induced colitis in rats and to confirm their short-term safety after 14 days of daily dosing. Rats were dosed daily orally with vehicle, Ambrotose or Advanced Ambrotose. On day six groups of rats received tap water or 5% Dextran Sulfate sodium. Ambrotose and Advanced Ambrotose significantly lowered the disease scores and partially prevented the shortening of colon length. An increase in monocyte count was induced by dextran sulfate sodium and inhibited by Ambrotose and Advanced Ambrotose. There were no observable adverse effects after 14-day daily doses. The mechanism of action of the formulations against DSS-induced colitis may be related to its effect on monocyte count

A Three-Component Gene Expression System and Its Application for Inducible Flavonoid Overproduction in Transgenic Arabidopsis thaliana

Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A) promoter, CBF3 (C-repeat Binding Factor 3) transcription factor and cpl1-2 (CTD phosphatase-like 1) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of Anthocyanin Pigment 1) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone

Analysis of high-identity segmental duplications in the grapevine genome

<p>Abstract</p> <p>Background</p> <p>Segmental duplications (SDs) are blocks of genomic sequence of 1-200 kb that map to different loci in a genome and share a sequence identity > 90%. SDs show at the sequence level the same characteristics as other regions of the human genome: they contain both high-copy repeats and gene sequences. SDs play an important role in genome plasticity by creating new genes and modeling genome structure. Although data is plentiful for mammals, not much was known about the representation of SDs in plant genomes. In this regard, we performed a genome-wide analysis of high-identity SDs on the sequenced grapevine (<it>Vitis vinifera</it>) genome (PN40024).</p> <p>Results</p> <p>We demonstrate that recent SDs (> 94% identity and >= 10 kb in size) are a relevant component of the grapevine genome (85 Mb, 17% of the genome sequence). We detected mitochondrial and plastid DNA and genes (10% of gene annotation) in segmentally duplicated regions of the nuclear genome. In particular, the nine highest copy number genes have a copy in either or both organelle genomes. Further we showed that several duplicated genes take part in the biosynthesis of compounds involved in plant response to environmental stress.</p> <p>Conclusions</p> <p>These data show the great influence of SDs and organelle DNA transfers in modeling the <it>Vitis vinifera </it>nuclear DNA structure as well as the impact of SDs in contributing to the adaptive capacity of grapevine and the nutritional content of grape products through genome variation. This study represents a step forward in the full characterization of duplicated genes important for grapevine cultural needs and human health.</p