Erosion and Water Quality as Modeled by Creams: A Case Study of the Sedlicky Catchment

In the process of verifying and validating the models of agricultural nonpoint source pollution at IIASA, a study was made of the Sedlicky brook (Bohemia, Czechoslovakia) case. The CREAMS model, verified at the Samsin research area (Czechoslovakia) has been used as the mathematical instrument. The validation results of the CREAMS model for the boundary conditions between the field level and the watershed level seem to show that under certain conditions, it can be applied to small watersheds. For large watersheds, modification of the hydrology submodel is necessary in order to describe the comprehensive hydrologic phenomena, particularly, the interflow and some of the subsurface flow

Procedures, Numerical Parameters and Coefficients of the Creams Model: Application and Verification in Czechoslovakia

Problems of agricultural nonpoint source pollution have been investigated by the Resources and Environment Area (Task 2) at IIASA. The CREAMS model has been used as a mathematical aid to arrive at an in-depth understanding of erosion and to predict its influence on agriculture. The CREAMS model was created using data from North America. Investigations of its general use and verification under various conditions were useful. This paper summarizes the results of the verification of this model in a research area in Czechoslovakia and focuses attention on certain points which must be carefully considered during application of this model

Novel bi- and trifunctional inhibitors of tumor-associated proteolytic systems

Serine proteases, cysteine proteases, and matrix metalloproteinases (MMPs) are involved in cancer cell invasion and metastasis. Recently, a recombinant bifunctional inhibitor (chCysuPA(19-31)) directed against cysteine proteases and the urokinasetype plasminogen activator (uPA)/plasmin serine protease system was generated by introducing the uPA receptor (uPAR)binding site of uPA into chicken cystatin (chCysWT). In the present study, we designed and recombinantly produced multifunctional inhibitors also targeting MMPs. The inhibitors comprise the Nterminal inhibitory domain of human TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) or TIMP-3, fused to chCysuPA(19-31) or chCysWT. As demonstrated by various techniques, these fusion proteins effectively interfere with all three targeted protease systems. In in vitro Matrigel invasion assays, the addition of recombinant inhibitors strongly reduced invasion of ovarian cancer cells (OVMZ-6\#8). Additionally, OVMZ 6\#8 cells were stably transfected with expression plasmids encoding the various inhibitors. Synthesis and secretion of the inhibitors was verified by a newly developed ELISA, which selectively detects the recombinant proteins. Invasive capacity of inhibitorproducing cells was significantly reduced compared to vectortransfected control cells. Thus, these novel, compact, and smallsize inhibitors directed against up to three different tumorassociated proteolytic systems may represent promising agents for prevention of tumor cell migration and metastasis