Appendix C. Summary E:P ratios for streams and lakes.

Summary E:P ratios for streams and lakes

Appendix E. Summary of insect dispersal studies derived from the literature.

Summary of insect dispersal studies derived from the literature

Appendix D. Estimates of annual emergence of aquatic insects from streams summarized from the literature.

Estimates of annual emergence of aquatic insects from streams summarized from the literature

Appendix A. Production values for lakes synthesized from the literature.

Production values for lakes synthesized from the literature

Appendix A. Mean zoobenthivory (as a percentage of gut content volume) of prey fish species consumed by piscivores.

Mean zoobenthivory (as a percentage of gut content volume) of prey fish species consumed by piscivores

Appendix G. Reference list for studies cited in Appendices A–E.

Reference list for studies cited in Appendices A–E

Food-chain length in lakes of different origins and age.

<p>A) Boxplot for food-chain length in lakes of different origins. Symbols represent the median FCL, boxes  =  inter-quartiles, and bars  =  maximum and minimum values. Different letters indicate significant differences by multiple comparison using ANOVA (p<0.001). B) Correlation between age of lake and food-chain length. Different symbols are for reservoir, glacial and ancient lakes (see A). Lake Victoria and Lake Kyoga are originally from 400,000 years ago, but are thought to have dried up 14,600 years ago.</p

Correlations between food-chain length, latitude, log₁₀ surface area (km²), log₁₀ volume (km³), mean depth (m), log₁₀ age of lake (year, the points of Lake Kyoga and Victoria were plotted as 400,000 year), and log₁₀ (endemic species number +1).

<p>Symbols mean the origin of lakes as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037856#pone-0037856-g001" target="_blank">Fig.1A</a>.</p

Supplement 1. Site-specific predictions of Orconectes rusticus establishment and vulnerability of O. propinquus and O. virilis in lakes and streams of Wisconsin.

<h2>File List</h2><blockquote> <p><a href="lakedata.txt">lakedata.txt</a></p> <p><a href="streamdata.txt">streamdata.txt</a></p> </blockquote><h2>Description</h2><blockquote> <p>The lakedata.txt file is a tab-separated ASCII file. </p> <p>Column definitions:</p> <ol> <li>WBIC: Water Body Indentification Codes assigned by the Wisconsin Department of Natural Resources (<a href="http://dnr.wi.gov/maps/gis/datahydro.html">http://dnr.wi.gov/maps/gis/datahydro.html</a>)</li> <li>Predicted O_RUSTICU: Predicted habitat suitability for <i>O. rusticus</i> (introduction and establishment)</li> <li>Vulnerability of O_PROPINQ: Vulnerability of <i>O. propinquus</i> to the combined probability of <i>O. rusticus</i> introduction, establishment, and the subsequent extirpation</li> <li>Vulnerability of O_VIRILIS: Vulnerability of <i>O. virilis</i> to the combined probability of <i>O. rusticus</i> introduction, establishment, and the subsequent extirpation</li> <li>Vulnerability of O_PROPINQ or O_VIRILIS: Vulnerability of <i>O. propinquus</i> or <i>O. virilis</i> to the combined probability of O. rusticus introduction, establishment, and the subsequent extirpation</li> </ol> <p>Checksum values are:</p> <blockquote> <p>Column B (Predicted O_RUSTICU): SUM = 109.5826399; 0 missing values; 4200 rows with data<br> Column C (Vulnerability of O_PROPINQ): SUM = 2.649128499; 0 missing values; 4200 rows with data<br> Column D (Vulnerability of O_VIRILIS): SUM = 50.52210568; 0 missing values; 4200 rows with data<br> Column E (Vulnerability of O_PROPINQ or O_VIRILIS): SUM = 53.17123418; 0 missing values; 4200 rows with data<br> </p> </blockquote> <p>The streamdata.txt file is a tab-separated ASCII file.</p> <p>Column definitions:</p> <ol> <li>PU_GAP_CODE Processing unit code for stream segments assigned by the Great Lakes Aquatic GAP Project (<a href="http://www.glsc.usgs.gov/GLGAP.htm">http://www.glsc.usgs.gov/GLGAP.htm</a>)</li> <li>Predicted O_RUSTICU: Predicted habitat suitability for <i>O. rusticus</i> (introduction and establishment)</li> <li>Vulnerability of O_PROPINQ: Vulnerability of <i>O. propinquus</i> to the combined probability of <i>O. rusticus</i> introduction, establishment, and the subsequent extirpation</li> <li>Vulnerability of O_VIRILIS: Vulnerability of <i>O. virilis</i> to the combined probability of <i>O. rusticus</i> introduction, establishment, and the subsequent extirpation</li> <li>Vulnerability of O_PROPINQ or O_VIRILIS: Vulnerability of <i>O. propinquus</i> or <i>O. virilis</i> to the combined probability of O. rusticus introduction, establishment, and the subsequent extirpation</li> </ol> <p>Checksum values are:</p> <blockquote> <p>Column B (Predicted O_RUSTICU): SUM = 9255.811205; 0 missing values; 35856 rows with data<br> Column C (Vulnerability of O_PROPINQ): SUM = 360.1863446; 0 missing values; 35856 rows with data<br> Column D (Vulnerability of O_VIRILIS): SUM = 671.4773124; 0 missing values; 35856 rows with data<br> Column E (Vulnerability of O_PROPINQ or O_VIRILIS): SUM = 1031.663657; 0 missing values; 35856 rows with data</p> </blockquote> </blockquote

Appendix A. Path diagrams and models output showing the direct and indirect effects of aquatic reliance (δ13C) and trophic position on MeHg.

Path diagrams and models output showing the direct and indirect effects of aquatic reliance (δ13C) and trophic position on MeHg