3 research outputs found
ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΌΠ΅ΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½Π΅Π²ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Π½Π° ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΡΡ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΡ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ² Π°ΡΠ΅Π»Π»ΡΠ»ΡΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΡΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΡΠΈΠΊΡΠ°
Acellular dermal matrices (ADMs) are gaining popularity as surgical materials for operations on the pelvic organs, as well as in burn therapy and plastic surgery. Evaluation of the biocompatibility of surgical materials is an important and necessary step in the development of new ADMs.The aim of the study was to compare the results of subcutaneous implantation of ADM and native porcine skin in rats.Materials and methods. To obtain ADMs, detergent β enzymatic decellularization was used. On days 7, 14, 21, and 60 after the implantation of ADMs (the experimental group) and native porcine skin (the control group), the animals were removed from the experiment. The histologic sections were stained with hematoxylin β eosin and Massonβs trichrome stain, then an immunohistochemical reaction with antibodies to CD3 and CD68 was performed. Computer morphometry was carried out using the ImageJ software.Results. On day 7 after the implantation, moderate sterile inflammation in the experimental group and pronounced sterile inflammation with eosinophil infiltration in the control group were observed. On day 14 of the experiment, the samples from the experimental group were characterized by a relatively low content of macrophages and T-lymphocytes with insignificant edema and no signs of ADM biodegradation. The control group showed pronounced inflammation, a large number of infiltrating macrophages and T lymphocytes, as well as fragmentation of collagen fibers. On day 21 of the experiment, a thin capsule was formed around ADM, there was a small number of infiltrating T lymphocytes and macrophages, the collagen fibers of the implant were intact. In the samples of the control group, there was pronounced inflammation with the presence of a significant number of lymphocytes and macrophages, as well as fragmentation and vascularization of the implant. On day 60 of the experiment, no inflammatory response was observed around ADM, biodegradation was minimal, and a dense fibrous capsule was formed around the fragment of the native porcine skin.Conclusion. The experimental ADM has low immunogenicity and a low degree of biodegradation, which makes it possible to use it for further research to create efficient surgical material that is safe for use in clinical practice.ΠΡΠ΅Π»Π»ΡΠ»ΡΡΠ½ΡΠ΅ Π΄Π΅ΡΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΌΠ°ΡΡΠΈΠΊΡΡ (ΠΠΠ) Π½Π°Π±ΠΈΡΠ°ΡΡ Π±ΠΎΠ»ΡΡΡΡ ΠΏΠΎΠΏΡΠ»ΡΡΠ½ΠΎΡΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠ² ΠΏΡΠΈ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΡΡ
Π½Π° ΠΎΡΠ³Π°Π½Π°Ρ
ΠΌΠ°Π»ΠΎΠ³ΠΎ ΡΠ°Π·Π°, Π² ΠΎΠΆΠΎΠ³ΠΎΠ²ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΈ ΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Ρ
ΠΈΡΡΡΠ³ΠΈΠΈ. ΠΡΠΎΠ²Π΅ΡΠΊΠ° Π±ΠΈΠΎΡΠΎΠ²ΠΌΠ΅ΡΡΠΈΠΌΠΎΡΡΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠ² ΡΠ²Π»ΡΠ΅ΡΡΡ Π²Π°ΠΆΠ½ΡΠΌ ΠΈ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΡΠΌ ΡΡΠ°ΠΏΠΎΠΌ ΠΏΡΠΈ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠ΅ Π½ΠΎΠ²ΡΡ
ΠΠΠ.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΏΡΠΎΠ²Π΅ΡΡΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΠΎΠΉ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ ΠΊΡΡΡΠ°ΠΌ ΠΠΠ ΠΈ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ Π΄Π΅ΡΠΌΡ ΡΠ²ΠΈΠ½ΡΠΈ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΠΠ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π΄Π΅ΡΠ΅ΡΠ³Π΅Π½ΡΠ½ΠΎ-ΡΠ½Π·ΠΈΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ Π΄Π΅ΡΠ΅Π»Π»ΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΠΈ. Π§Π΅ΡΠ΅Π· 7, 14, 21, 60 ΡΡΡ ΠΏΠΎΡΠ»Π΅ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠΈΠΈ ΠΠΠ (ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π°Ρ Π³ΡΡΠΏΠΏΠ°) ΠΈ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ Π΄Π΅ΡΠΌΡ ΡΠ²ΠΈΠ½ΡΠΈ (ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ Π³ΡΡΠΏΠΏΠ°) ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π²ΡΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΈΠ· ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°. ΠΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ΅Π·Ρ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π»ΠΈ Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½ΠΎΠΌ ΠΈ ΡΠΎΠ·ΠΈΠ½ΠΎΠΌ, ΡΡΠΈΡ
ΡΠΎΠΌΠΎΠΌ ΠΏΠΎ ΠΠ°ΡΡΠΎΠ½Ρ, Π²ΡΠΏΠΎΠ»Π½ΡΠ»ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΡΡ ΡΠ΅Π°ΠΊΡΠΈΡ Ρ Π°Π½ΡΠΈΡΠ΅Π»Π°ΠΌΠΈ ΠΊ CD3 ΠΈ CD68. ΠΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΡΡ ΠΌΠΎΡΡΠΎΠΌΠ΅ΡΡΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ ImageJ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ° 7-Π΅ ΡΡΡ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΎΡΡ ΡΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠ΅ Π°ΡΠ΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅, Π² ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ β Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ Π°ΡΠ΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅ Ρ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»Π°ΠΌΠΈ Π² ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠ΅. ΠΠ° 14-Π΅ ΡΡΡ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π½ΠΈΠ·ΠΊΠΎΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΈ Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² Ρ Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΠΎΡΠ΅ΠΊΠΎΠΌ, Π±Π΅Π· Π±ΠΈΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ ΠΠΠ. Π ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ Π²ΡΡΠ²Π»Π΅Π½ΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅, ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΡ Π±ΠΎΠ»ΡΡΠΈΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎΠΌ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΈ Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ², Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ°ΡΠΈΡ ΠΊΠΎΠ»Π»Π°Π³Π΅Π½ΠΎΠ²ΡΡ
Π²ΠΎΠ»ΠΎΠΊΠΎΠ½. ΠΠ° 21-Π΅ ΡΡΡ Π²ΠΎΠΊΡΡΠ³ ΠΠΠ ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π»Π°ΡΡ ΡΠΎΠ½ΠΊΠ°Ρ ΠΊΠ°ΠΏΡΡΠ»Π°, Π² ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠ΅ ΠΌΠ°Π»ΠΎΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ Π’-Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΈ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ², ΠΊΠΎΠ»Π»Π°Π³Π΅Π½ΠΎΠ²ΡΠ΅ Π²ΠΎΠ»ΠΎΠΊΠ½Π° ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ° Π±ΡΠ»ΠΈ ΠΈΠ½ΡΠ°ΠΊΡΠ½Ρ. Π ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ β Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅ Ρ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ² ΠΈ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ², ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ°ΡΠΈΡ ΠΈ Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΡ ΠΈΠΌΠΏΠ»Π°Π½ΡΠ°ΡΠ°. ΠΠ° 60-Π΅ ΡΡΡ Π²ΠΎΠΊΡΡΠ³ ΠΠΠ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Π½Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΎΡΡ, Π±ΠΈΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΡ Π±ΡΠ»Π° ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΉ, Π²ΠΎΠΊΡΡΠ³ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ Π΄Π΅ΡΠΌΡ ΡΠ²ΠΈΠ½ΡΠΈ ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π»Π°ΡΡ ΠΏΠ»ΠΎΡΠ½Π°Ρ ΡΠΈΠ±ΡΠΎΠ·Π½Π°Ρ ΠΊΠ°ΠΏΡΡΠ»Π°.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΉ ΠΠΠ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π½ΠΈΠ·ΠΊΠΎΠΉ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π΅Π½Π½ΠΎΡΡΡΡ ΠΈ ΠΌΠ°Π»ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ Π±ΠΈΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΠΈ. ΠΡΠΎ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ Π΄Π°Π½Π½ΡΡ ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ Π΄Π»Ρ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΏΠΎ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΏΠΎΠ»Π½ΠΎΡΠ΅Π½Π½ΠΎΠ³ΠΎ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°, Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΠΎΠ³ΠΎ Π΄Π»Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠ΅
Atopic dermatitis in children: prospects of using innovation products as an external therapy
Goal of the research: to determine the efficacy of a new line of Atopic cosmetic products for skin care in children suffering from atopic dermatitis. Materials and methods: The study involved three groups of 15 children aged 3-12 suffering from atopic dermatitis in each group. Subjects from Group 1 were treated with Atopic Soothing Cream, from Group 2 with Atopic Soothing Stick Cream and from Group 3 with Atopic Everyday Care Cream. Different inclusion and exclusion criteria were developed for each group, and the algorithm for the product application and efficacy criteria were determined. According to recommendations, Atopic Soothing Cream and Atopic Soothing Stick Cream were applied to clean and dry skin in the morning and in the evening for the study period of 14 days. The patientsβ skin condition was assessed prior to the study and on Day 14 of the therapy; the assessment covered parameters necessary for calculating the SCORAD Index. Atopic Everyday Care Cream was applied to clean and dry skin in the morning and in the evening for 28 days. The patientsβ skin condition was assessed prior to the study and on Days 14 and 28 of the therapy including the SCORAD Index and assessments of the skin hydration level of the epidermal corneous layer and transepidermal waster loss (TEWL) using the DermaLabCombo CORTEX device. According to the study results, the line of Atopic cosmetic products fully complies with the properties specified by the manufacturer, reliably improves the skin condition in children suffering from atopic dermatitis, has no irritant or sensitizing effect, and can be recommended for the complex treatment and rehabilitation of patients suffering from the pathology
ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄Π΅ΡΠΌΡ ΡΠ²ΠΈΠ½ΡΠΈ Π΄Π»Ρ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠ½ΡΡ ΡΠ°Π½
Aim. To study the efficacy of dermal hydrogel application in the experimental treatment of superficial scarified wounds in rats.Materials and methods. The hydrogel was obtained from porcine dermis by alkaline hydrolysis. The DNA concentration was determined using the Nano Drop ND-1000 spectrophotometer. The study included 30 male Sphinx rats. Scarified wounds were created on the rat skin, then the rats were divided into two groups: group 1 β rats without treatment, or control group (n = 15), group 2 β rats with wound treatment with the dermal hydrogel for 5 days, or experimental group (n = 15). On day 3, 7, and 14 of the experiment, we explanted skin samples from the wound area and performed routine H&E staining.Results. On day 3 of the experiment, moderate inflammation, edema, and collagen fiber disorganization were revealed in the experimental group, and pronounced inflammation with purulent exudate was found in the control group. On day 7 of the experiment, inflammation and foci of stratified epithelium were detected in the control group. The histologic analysis of the skin samples from the experimental group showed pronounced plethora of the vessels, necrotic changes of the dermis, and edema. The total thickness of the epidermis and the thickness of its stratum corneum were greater than in the control group samples. On day 14, the differences between the groups were minimal and the epidermis was thickened in the experimental group animals.Conclusion. The study examined the effects of the dermal hydrogel on scarified wounds in rats. We found faster skin regeneration (by 1.5β2 days) in the experimental group compared to the controls. Besides, the rats of the experimental group were characterized by an increase in the number of fibroblasts in the dermis and thickened epidermis in the affected area.Π¦Π΅Π»Ρ β ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π΄Π΅ΡΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΏΡΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΌ Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΡΡ
ΡΠΊΠ°ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠ°Π½ Ρ ΠΊΡΡΡ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΈΠ· ΡΠ²ΠΈΠ½ΠΎΠΉ Π΄Π΅ΡΠΌΡ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅Π»ΠΎΡΠ½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°. Π ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΠΠ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠ° Nano Drop ND-1000. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π½Π° 30 ΡΠ°ΠΌΡΠ°Ρ
ΠΊΡΡΡ ΠΏΠΎΡΠΎΠ΄Ρ ΡΡΠΈΠ½ΠΊΡ. ΠΡΡΡΠ°ΠΌ Π½Π°Π½ΠΎΡΠΈΠ»ΠΈ ΡΠΊΠ°ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΡΠ°Π½Ρ, Π·Π°ΡΠ΅ΠΌ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅ Π±ΡΠ»ΠΈ ΡΠ°Π·Π΄Π΅Π»Π΅Π½Ρ Π½Π° Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ: Π³ΡΡΠΏΠΏΠ° 1 β Π±Π΅Π· Π»Π΅ΡΠ΅Π½ΠΈΡ, ΠΈΠ»ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ Π³ΡΡΠΏΠΏΠ° (n = 15), Π³ΡΡΠΏΠΏΠ° 2 β Π»Π΅ΡΠ΅Π½ΠΈΠ΅ ΡΠ°Π½Ρ Π΄Π΅ΡΠΌΠ°Π»ΡΠ½ΡΠΌ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Π΅ΠΌ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 5 ΡΡΡ (n = 15). ΠΠ° 3-ΠΈ, 7-Π΅ ΠΈ 14-Π΅ ΡΡΡ ΡΠΊΡΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π»ΠΈΡΡ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΊΠΎΠΆΠΈ ΠΈΠ· ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠ°Π½Ρ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°Π»ΠΈΡΡ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ° 3-ΠΈ ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΊΠΎΠΆΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΡ 2 ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΎΡΡ ΡΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅ Ρ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΡΠΌ ΠΎΡΠ΅ΠΊΠΎΠΌ ΠΈ Π΄ΠΈΡΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°ΡΠΈΠ΅ΠΉ ΠΊΠΎΠ»Π»Π°Π³Π΅Π½ΠΎΠ²ΡΡ
Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, Π° ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ β Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅ Ρ Π³Π½ΠΎΠΉΠ½ΡΠΌ ΡΠΊΡΡΡΠ΄Π°ΡΠΎΠΌ. ΠΠ° 7-Π΅ ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° Ρ ΠΊΡΡΡ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΈ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΠΎΡΠ°Π³ΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΌΠ½ΠΎΠ³ΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΠ΅Π»ΠΈΡ. ΠΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΊΠΎΠΆΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΡ 2 ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π» Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ ΠΏΠΎΠ»Π½ΠΎΠΊΡΠΎΠ²ΠΈΠ΅ ΡΠΎΡΡΠ΄ΠΎΠ², Π½Π΅ΠΊΡΠΎΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π΄Π΅ΡΠΌΡ ΠΈ Π΅Π΅ ΠΎΡΠ΅ΠΊ. ΠΠ±ΡΠ°Ρ ΡΠΎΠ»ΡΠΈΠ½Π° ΡΠΏΠΈΠ΄Π΅ΡΠΌΠΈΡΠ° ΠΈ ΡΠΎΠ»ΡΠΈΠ½Π° Π΅Π³ΠΎ ΡΠΎΠ³ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ Π±ΡΠ»Π° Π±ΠΎΠ»ΡΡΠ΅, ΡΠ΅ΠΌ Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ. ΠΠ° 14-Π΅ ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° ΡΠ°Π·Π»ΠΈΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΠΌΠΈ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ Π±ΡΠ»ΠΈ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½Ρ, ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΡΡΠΎΠ»ΡΠ΅Π½ΠΈΠ΅ ΡΠΏΠΈΠ΄Π΅ΡΠΌΠΈΡΠ° Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΡ 2 ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄Π΅ΡΠΌΡ ΡΠ²ΠΈΠ½ΡΠΈ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠΊΠ°ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠ°Π½ ΠΊΡΡΡ ΠΏΠΎΠ»Π½ΠΎΠ΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠΆΠΈ Π² ΠΏΠΎΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π½Π°ΡΡΡΠΏΠ°Π»ΠΎ Π½Π° 1,5β2 ΡΡΡ Π±ΡΡΡΡΠ΅Π΅, ΡΠ΅ΠΌ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅. ΠΠΎΠΌΠΈΠΌΠΎ ΡΡΠΎΠ³ΠΎ Π±ΡΠ»ΠΎ Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠΎΠ² Π² Π΄Π΅ΡΠΌΠ΅ ΠΈ ΡΡΠΎΠ»ΡΠ΅Π½ΠΈΠ΅ ΡΠΏΠΈΠ΄Π΅ΡΠΌΠΈΡΠ° ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π°Π½Π°Π»ΠΎΠ³ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Ρ ΠΊΡΡΡ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ