Survival of different <i>rif-nal</i> strains on MMC plates.

<p>a. % survival of relevant <i>gyrA87-</i>Rif^R strains on LB plates containing 0.5 µg/ml of MMC. b. % survival of relevant <i>rpoB87-</i>Nal^R strains on LB plates containing 0.5 µg/ml of MMC. The values plotted are the average of three independent set of experiments. The standard error values are given as error bars. The % survival values were calculated as (cfu per ml in LB with MMC plates/cfu per ml in LB plates w/o MMC) X100.</p

<i>rpoB</i> alleles used in this study and their relevant characteristics.

*<p>Values given as fold increase in termination read through are from Jin <i>et al</i>, 1988.</p

Level of MMC survival in relevant strains and its implications.

<p>R (+++) – ∼100% survival.</p><p>R (++) – ∼1% survival.</p><p>S (−) – ∼0.01–0.001% survival.</p><p>S (–) – ∼ 0.0001–0.00001% survival.</p><p>S (–) – Complete loss of survival.</p

List of <i>E. coli</i> K12 strains used in this study, their relevant Genotype and source.

*<p>CGSC -Coli Genetic Stock Centre, USA.</p><p><b>#</b> CDFD – Centre for DNA Fingerprinting and Diagnostics.</p

Sequential spotting test for analyses of survival of various Rif-Nal strains on LB plates containing 0.5 µg/ml of MMC after ∼24 hours incubation.

<p>Appropriate control strains (AB1157 for positive control and DM49 for Negative control) were also tested. The growth of the respective strains on LB plates without MMC after ∼12–14 hours incubation is given on the right.</p

Extent of filamentation in MMC untreated and 0.5 µg/ml MMC treated samples of indicated strains as observed under light microscope.

<p>Relevant genotypes of the strains are mentioned wherever appropriate.</p