EST2Prot: Mapping EST sequences to proteins

BACKGROUND: EST libraries are used in various biological studies, from microarray experiments to proteomic and genetic screens. These libraries usually contain many uncharacterized ESTs that are typically ignored since they cannot be mapped to known genes. Consequently, new discoveries are possibly overlooked. RESULTS: We describe a system (EST2Prot) that uses multiple elements to map EST sequences to their corresponding protein products. EST2Prot uses UniGene clusters, substring analysis, information about protein coding regions in existing DNA sequences and protein database searches to detect protein products related to a query EST sequence. Gene Ontology terms, Swiss-Prot keywords, and protein similarity data are used to map the ESTs to functional descriptors. CONCLUSION: EST2Prot extends and significantly enriches the popular UniGene mapping by utilizing multiple relations between known biological entities. It produces a mapping between ESTs and proteins in real-time through a simple web-interface. The system is part of the Biozon database and is accessible at

GABA Withdrawal Modifies Network Activity in Cultured Hippocampal Neurons

Dissociated hippocampal neurons, grown in culture for 2 to 3 weeks, tended to fire bursts of synaptic currents at fairly regular intervals, representing network activity. A brief exposure of cultured neurons to GABA caused a total suppression of the spontaneous network activity. Following a washout of GABA, the activity was no longer clustered in bursts and instead, the cells fired at a high rate tonic manner. The effect of removing GABA could be seen as long as 1 to 2 days after GABA withdrawal and is expressed as an increase in the number of active cells in a network, as well as in their firing rates. Such striking effects of GABA removal may underlie part of the GABA withdrawal syndrome seen elsewhere

Designing a physical activity parenting course : parental views on recruitment, content and delivery

Background Many children do not engage in sufficient levels of physical activity (PA) and spend too much time screen-viewing (SV). High levels of SV (e.g. watching TV, playing video games and surfing the internet) and low levels of PA have been associated with adverse health outcomes. Parenting courses may hold promise as an intervention medium to change children’s PA and SV. The current study was formative work conducted to design a new parenting programme to increase children’s PA and reduce their SV. Specifically, we focussed on interest in a course, desired content and delivery style, barriers and facilitators to participation and opinions on control group provision. Methods In-depth telephone interviews were conducted with thirty two parents (29 female) of 6–8 year olds. Data were analysed thematically. An anonymous online survey was also completed by 750 parents of 6–8 year old children and descriptive statistics calculated. Results Interview participants were interested in a parenting course because they wanted general parenting advice and ideas to help their children be physically active. Parents indicated that they would benefit from knowing how to quantify their child’s PA and SV levels. Parents wanted practical ideas of alternatives to SV. Most parents would be unable to attend unless childcare was provided. Schools were perceived to be a trusted source of information about parenting courses and the optimal recruitment location. In terms of delivery style, the majority of parents stated they would prefer a group-based approach that provided opportunities for peer learning and support with professional input. Survey participants reported the timing of classes and the provision of childcare were essential factors that would affect participation. In terms of designing an intervention, the most preferred control group option was the opportunity to attend the same course at a later date. Conclusions Parents are interested in PA/SV parenting courses but the provision of child care is essential for attendance. Recruitment is likely to be facilitated via trusted sources. Parents want practical advice on how to overcome barriers and suggest advice is provided in a mutually supportive group experience with expert input

Random Amplified Polymorphic DNA Analysis in Section \u3cem\u3ePnigma\u3c/em\u3e of the Genus \u3cem\u3eBromus\u3c/em\u3e L.

The section Pnigma consists of about 60 species. It was shown that Eurasian species of the section with the same ploidy level have similar nuclear DNA contents (Tuna et al., 2001). A linear correlation between nuclear DNA content and ploidy level was also found (Tuna et al., 2001). Furthermore, karyotypes of Eurasian species are similar (Tuna et al., 2004a) but differ from the North American species (Tuna et al., 2004b). Yet, the genetic relationship among species within the section is poorly known (Armstrong, 1991). The objective of this study was to assess the phylogenetic relationships among species of section Pnigma by using the RAPD technique

Geographic Dependence of the Solar Irradiance Spectrum at Intermediate to High Frequencies

Temporal fluctuations in the solar irradiance are important for renewable-energy harvesting, as well as for climate and ecological-systems analysis. Environmental studies usually focus on slow fluctuations of the solar irradiance, assuming that fast fluctuations are averaged out, while studies of solar power generation emphasize the role of faster fluctuations, due to their effect on grid stability. Several analyses have reported a power-law dependence of the generated-power spectrum for frequencies ranging from 1/min to 1/year. However, both the origin of this power law and its geographic dependence are not fully understood, an issue of significance when one considers smoothing of solar-photovoltaic-power fluctuations by combining geographically distributed generation sources. Here we show that the power law appears in the global and direct components of the solar radiation and not only in the generated power. We also show that the exponent of the power law has a clear latitudinal dependence. At most locations, the spectral power-law dependence of the intermediate (1/day1/h) frequencies. Using a simple model, we explain the origin of the power-law spectra and the latitudinal dependence. The implications of the power law for photovoltaic power generation and grid stability are discussed. We also suggest that the analysis of solar power generation should consider the power spectrum of the clear-sky irradiance at the location of interest in order to separate atmospheric effects from geographical ones