172 research outputs found
Cutaneous sinus histiocytosis of face of the non-Langerhans cell histiocytoses type (cutaneous Rosai β Dorfman disease)
An extremely rare case of sinus histiocytosis of the non-Langerhans cell histiocytoses type is described in 55-year-old patient with isolated localization of cutanious lesion on the right cheek without involvement of nodular structures. Main skin lesion was an erythematous-cyanotic node with multiple milia-like lesions on the top of it. Histologically revealed feature was a large number of plasma cells, besides phenomenon of emperipolesis was described, which was first falsely perceived by pathologists as phagocytosis. In immu-nohistochemistry assay the changes were characterized by proliferation of large polygonal histiocytes with accumulation of pentalamellar markers in its cytoplasm (protein S-100, CD68)
Skeletal Muscle-Specific Ablation of Ξ³cyto-Actin Does Not Exacerbate the mdx Phenotype
We previously documented a ten-fold increase in Ξ³cyto-actin expression in dystrophin-deficient skeletal muscle and hypothesized that increased Ξ³cyto-actin expression may participate in an adaptive cytoskeletal remodeling response. To explore whether increased Ξ³cyto-actin fortifies the cortical cytoskeleton in dystrophic skeletal muscle, we generated double knockout mice lacking both dystrophin and Ξ³cyto-actin specifically in skeletal muscle (ms-DKO). Surprisingly, dystrophin-deficient mdx and ms-DKO mice presented with comparable levels of myofiber necrosis, membrane instability, and deficits in muscle function. The lack of an exacerbated phenotype in ms-DKO mice suggests Ξ³cyto-actin and dystrophin function in a common pathway. Finally, because both mdx and ms-DKO skeletal muscle showed similar levels of utrophin expression and presented with identical dystrophies, we conclude utrophin can partially compensate for the loss of dystrophin independent of a Ξ³cyto-actin-utrophin interaction
Practical recommendations for safe reduction of body weight in combat sports: scoping review
The presence of weight categories in martial arts requires an athlete to constantly maintain and control body weight and, in some cases, to reduce it. For this reason, it is common among martial artists to use forced methods of body weight reduction, which are often unphysiological and may have a negative impact on performance, as well as pose a danger to health. In this connection, it is of practical interest to search, analyse and introduce into practice physiological and safe methods of body weight reduction by athletes-athletes before participation in competitions. The aim of the study is to evaluate the safety and effectiveness of various methods of weight loss in athletes preparing to participate in competitions.Materials and methods: the review was conducted according to the methodology of Scoping review. Articles were selected in the domestic data-bases eLibrary and Russian State Library, using the following keywords: Β«ΠΌΠ΅ΡΠΎΠ΄Ρ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° ΠΠΠ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° ΠΠΠ Π²Π΅ΡΠΎΠ³ΠΎΠ½ΠΊΠ°Β» Π Β«Π΅Π΄ΠΈΠ½ΠΎΠ±ΠΎΡΡΡΠ²Π° ΠΠΠ Π±ΠΎΠ΅Π²ΡΠ΅ Π²ΠΈΠ΄Ρ ΡΠΏΠΎΡΡΠ°Β», as well as in the foreign databases ScienceDirect and PubMed, using the following keywords: Β«making weight OR weight lossΒ» AND Β«combat sportsΒ», placed in a search depth of 15 years.Results: we found 121 studies, 3 duplicates were excluded after initial screening, 118 studies were screened for inclusion criteria, and a total of 16 studies were included in the review.Conclusion: a reduction of 5 to 10 % of body weight less than 7 days before the official weigh-in may adversely affect performance parameters and the state of the bodyβs functional systems. A reduction of up to 5 % of body weight 7 or more days before the official weigh-in is the safest
Practical guidelines for standardising the measurement of resting metabolism by indirect calorimetry: a literature review
Accurate resting metabolic rate readings are essential for dietary planning and body composition monitoring not only for healthy individuals but also for athletes. A number of factors can alter resting metabolic rate during its measurement by indirect calorimetry. The methodology used may affect the results of the study. A clear standardisation of this procedure is needed to obtain the most accurate results.Purpose: To review the literature to determine the optimal subject condition and methodology for the resting metabolism measurement procedure using indirect calorimetry.Materials and methods: A literature search was conducted in PubMed, MEDLINE and Cochrane Library databases. The query included key words and logical phrases: βcalorimetryβ, βindirect calorimetryβ, βresting metabolic rateβ, βenergy metabolismβ, βbasal metabolismβ, βstandardsβ. Only Englishlanguage studies and human studies were considered. Additional information was identified because of the review and included in the review.Results: the parameters of standardization during the resting metabolism measurement procedure are described: consumption of food, ethanol, caffeine, nicotine; daily activities and physical activity; body position in space and environmental conditions during the measurement; actions of the specialist performing the procedure, etc. The article outlines effective methods for measuring resting metabolism to obtain the most accurate results in both athletes and non-athletes.Conclusion: an attempt has been made to formulate precise methodological rules for standardization and recommendations for measuring resting metabolism by indirect calorimetry
Clinical application of induced sputum in children with newly diagnosed asthma: cellular and immunologic characteristics
Authors investigate cells and immunologic factors of induced sputum in children with newly diagnosed asthma and healthy children without atopy. The aim of the study was to find out the differences of cellular and immunologic profiles of induced sputum in children with newly diagnosed asthma. 35 children aged 1,5-5 years old (Me = 3,5 years) were include in this study: 18 children with newly diagnosed asthma and 17 children (control group) without allergic diseases, which had no respiratory symptoms during last month. Sputum induction carried out according to our modification of protocol developed by Pin et al. The levels of IgE, slgA, lgG4, IL-1Ξ², IL-4, IL-8, IL-13, TNFΞ±, INFΞ³, N03, NOX and cells percentage (macrophages, neutrophils, eosinophils, lymphocytes) were evaluated in sputum. Results. The percentage of eosinophils was significantly higher and the percentage of macrophages was significantly lower in induced sputum of children with newly diagnosed asthma. The levels of proinflammatory factors (IL-1Ξ², IL-4, IL-8, IL-13, TNFΞ±), immunoglobulins, which participate in allergic inflammation (IgE, slgA, lgG4) and stable metabolites of NO (NO3, NOX) in sputum were also significantly higher in children with newly diagnosed asthma.ΠΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΡΡ ΠΏΠΎΠ΄ΡΡΠ΅Ρ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π² ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΏΠ΅ΡΡΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ ΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ. Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΎ Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠΈΡΠΎΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ Ρ Π΄Π΅ΡΠ΅ΠΉ ΡΠ°Π½Π½Π΅Π³ΠΎ Π²ΠΎΠ·ΡΠ°ΡΡΠ° Ρ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΈΠ½ΡΠ»ΠΈ ΡΡΠ°ΡΡΠΈΠ΅ 35 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ 1,5-5 Π»Π΅Ρ (ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ 3,5Π³.): 18 Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ ΠΈ 17 ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ - ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ Π³ΡΡΠΏΠΏΠ°. Π£ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ Π² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠ΅Π΄ΡΠ΅ΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΠΌΠ΅ΡΡΡΠ° Π½Π΅ Π±ΡΠ»ΠΎ Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΡΠΏΠΈΠ·ΠΎΠ΄ΠΎΠ² ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. ΠΠ½Π΄ΡΠΊΡΠΈΡ ΠΌΠΎΠΊΡΠΎΡΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ ΠΏΠΎ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΌΡ Π½Π°ΠΌΠΈ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Ρ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π³ΠΈΠΏΠ΅ΡΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ° Ρ
Π»ΠΎΡΠΈΠ΄Π° Π½Π°ΡΡΠΈΡ. ΠΡΠΎΡΠΎΡΠΈΠΏΠΎΠΌ ΡΠ²ΠΈΠ»ΡΡ ΠΌΠ΅ΡΠΎΠ΄, ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½ΡΠΉ Pin et al., ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΡΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΉ ΡΠΎΡΡΠ°Π² (%) (ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΈ, Π½Π΅ΠΉΡΡΠΎΡΠΈΠ»Ρ, ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»Ρ, Π»ΠΈΠΌΡΠΎΡΠΈΡΡ) ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΡΠΎΡΠΈΠ»Ρ (IgE, slgA, lgG4, IL-1Ξ², IL-4, IL-8, IL-13. TNFΞ±, INFΞ³, NO3, NOX) ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ Π΄Π΅ΡΠ΅ΠΉ Ρ Π²ΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π½ΠΎΠΉ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ Π² ΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠΎΠΊΡΠΎΡΠ΅ ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΎΠ·ΠΈΠ½ΠΎΡΠΈΠ»ΠΎΠ² (%) Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π²ΡΡΠ΅, Π° ΡΡΠΎΠ²Π΅Π½Ρ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² (%) Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π½ΠΈΠΆΠ΅ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΡΠΎ Π·Π΄ΠΎΡΠΎΠ²ΡΠΌΠΈ Π΄Π΅ΡΡΠΌΠΈ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ. Π’Π°ΠΊ ΠΆΠ΅ Ρ Π΄Π΅ΡΠ΅ΠΉ Ρ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠΎΠΉ Π²ΡΡΠ²Π»Π΅Π½Ρ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (Π€ΠΠΠ°, ΠΠ 4, IL-1B, ΠΠ8, ΠΠ 13), ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½ΠΎΠ² (IgE, lgG4) ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ
Π² Π°Π»Π»Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠΈ Π±ΡΠΎΠ½Ρ
ΠΎΠ² ΠΏΡΠΈ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΠ΅ ΠΈ ΠΊΠΎΠ½Π΅ΡΠ½ΡΡ
ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΡΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠΎΠ² ΠΎΠΊΡΠΈΠ΄Π° Π°Π·ΠΎΡΠ° (NO3, NOX) ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄Π΅ΡΠ΅ΠΉ Π±Π΅Π· Π°ΡΠΎΠΏΠΈΠΈ
Plant and animal protein for muscle mass and strength gains: a systematic review
Background: Controversial approaches to studying the effect of proteins of various origins on the functional parameters of human skeletal muscles have led to the erroneous opinion that plant-based protein is a lesser stimulator of muscle mass growth and muscle strength than animal protein. Therefore, the purpose of this systematic review was to actually evaluate the effects of plant and animal protein intake on muscle mass and strength through studies comparing protein supplements.Methods: Literature search was carried out in the databases PubMed, Research Gate and the database of the Russian State Library. Studies were considered both in English and in Russian, over the past 20 years, filtered by date: from July 2002 to July 2022. Inclusion Criteria: Participants are healthy men and women over 18 years of age; taking protein supplements from plant products (soy, wheat, pea, rice, etc.); comparison with a group taking protein supplements from animal products (whey, beef, egg, etc.); studies assessed lean and/or muscle mass and assessed participants' muscle strength before and after supplementation; randomized controlled trial.Results: A total of 970 studies were found. After initial screening for title and abstract, 938 studies were excluded. Of the 32 publications selected, 5 duplicates were excluded, and after a secondary selection, 18 studies that did not meet the PICOS criteria were excluded. As a result of the search and selection, the review included 9 publications.Conclusions: Athletes and active individuals who prefer to consume plant-based protein products may not be inferior in terms of muscle strength and muscle mass to those athletes who prefer animal-based protein products. To further explore this topic, more randomized controlled trials should be conducted, taking into account the requirements for standardization and with a large number of participants.Registration: PROSPERO 2022 CRD4202234524
Practical guidelines for standardising the measurement of resting metabolism by indirect calorimetry: a literature review
Accurate resting metabolic rate readings are essential for dietary planning and body composition monitoring not only for healthy individuals but also for athletes. A number of factors can alter resting metabolic rate during its measurement by indirect calorimetry. The methodology used may affect the results of the study. A clear standardisation of this procedure is needed to obtain the most accurate results.Purpose: To review the literature to determine the optimal subject condition and methodology for the resting metabolism measurement procedure using indirect calorimetry.Materials and methods: A literature search was conducted in PubMed, MEDLINE and Cochrane Library databases. The query included key words and logical phrases: βcalorimetryβ, βindirect calorimetryβ, βresting metabolic rateβ, βenergy metabolismβ, βbasal metabolismβ, βstandardsβ. Only English-language studies and human studies were considered. Additional information was identified because of the review and included in the review.Results: the parameters of standardization during the resting metabolism measurement procedure are described: consumption of food, ethanol, caffeine, nicotine; daily activities and physical activity; body position in space and environmental conditions during the measurement; actions of the specialist performing the procedure, etc. The article outlines effective methods for measuring resting metabolism to obtain the most accurate results in both healthy individuals and athletes.Conclusion: an attempt has been made to formulate precise methodological rules for standardisation and recommendations for measuring resting metabolism by indirect calorimetry
Application of whey of Mozzarella di Bufala Campana fermented by lactic acid bacteria as a bread biopreservative agent
A total of nine isolated lactic acid bacteria (LAB) from tomato and sourdough with antifungal activity were employed to revaluate the whey of Mozzarella di Bufala through the fermentation process for 72 h at 37 Β°C. Then, the fermented whey (BWF) was characterised and used as biopreservative in bread formulation. L. plantarum TR7 and L. plantarum TR2 strains showed average lactic acid concentration in BWF of 13.8 g L 1. Also, the bread volatile organic compounds (VOC) analysis showed an increase in hexanal, benzeneacetaldehyde, benzaldehyde and pyrazine tetramethyl when using BWF as ingredient. Moreover, the DPPH-inhibitory activity of bread with BWF extract also reflected a 33% rise in comparison with control bread. The application of BWF as a biopreservation agent in bread showed an increase in shelf life compared with bread with 0.3% calcium propionate and bread control for 2 and 15 days, respectively. BWF can be used as an interesting biopreservation strategy of bread
ΠΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡ ΡΠ΅ΡΠ΄ΡΠ° ΠΏΡΠΈ COVID-19
Cardiovascular system is a significant target for SARS-CoV-2.The objective was to study morphological changes in the heart in patients who died from COVID-19.Materials and methods. Autopsy material of the heart of those who died from COVID-19 (700 autopsies) was analyzed. The analysis of autopsy protocols was carried out, macroscopic changes were assessed, histological preparations stained with hematoxylin and eosin, van Gieson stain, Schiffβs reagent, the results of immunohistochemistry with antibodies to CD68, CD3, CD4, CD8, CD45 were analyzed.Results. The leading pathomorphological changes in the heart in COVID-19 are acute discirculatory changes with predominant disorders in small intramyocardial vessels. Lymphocytic myocarditis was confirmed in 1 % of cases.Conclusion. Damage to cardiomyocytes in COVID-19 is multifactorial and associated with manifestations of the underlying cardiovascular pathology, changes in intramyocardial vessels due to the action of SARS-CoV-2, and hypoxia due to progressive lung damage.Π‘Π΅ΡΠ΄Π΅ΡΠ½ΠΎ-ΡΠΎΡΡΠ΄ΠΈΡΡΠ°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ Π·Π½Π°ΡΠΈΠΌΠΎΠΉ ΠΌΠΈΡΠ΅Π½ΡΡ SARS-CoV-2.Π¦Π΅Π»Ρ β ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π² ΡΠ΅ΡΠ΄ΡΠ΅ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
, ΡΠΌΠ΅ΡΡΠΈΡ
ΠΎΡ COVID-19.ΠΠ΅ΡΠΎΠ΄Ρ ΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ. ΠΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½ Π°ΡΡΠΎΠΏΡΠΈΠΉΠ½ΡΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» ΡΠ΅ΡΠ΄ΡΠ° ΡΠΌΠ΅ΡΡΠΈΡ
ΠΎΡ COVID-19 (700 Π°ΡΡΠΎΠΏΡΠΈΠΉ).ΠΡΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΎΠ°Π½Π°ΡΠΎΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠΎΠΊΠΎΠ»ΠΎΠ², ΠΎΡΠ΅Π½Π΅Π½Ρ ΠΌΠ°ΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ, ΠΏΠ΅ΡΠ΅ΡΠΌΠΎΡΡΠ΅Π½Ρ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ, ΠΎΠΊΡΠ°ΡΠ΅Π½Π½ΡΠ΅ Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½ΠΎΠΌ ΠΈ ΡΠΎΠ·ΠΈΠ½ΠΎΠΌ, ΠΏΠΎ ΠΠ°Π½ ΠΠΈΠ·ΠΎΠ½Ρ, ΡΠ΅Π°ΠΊΡΠΈΠ²ΠΎΠΌ Π¨ΠΈΡΡΠ°, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈΠΌΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΠΈ Ρ Π°Π½ΡΠΈΡΠ΅Π»Π°ΠΌΠΈ ΠΊ CD68, CD3, CD4, CD8, CD45.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ΅Π΄ΡΡΠΈΠΌΠΈ ΠΏΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΠΌΠΈ Π² ΡΠ΅ΡΠ΄ΡΠ΅ ΠΏΡΠΈ COVID-19 ΡΠ²Π»ΡΡΡΡΡ ΠΎΡΡΡΡΠ΅ Π΄ΠΈΡΡΠΈΡΠΊΡΠ»ΡΡΠΎΡΠ½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Ρ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΠΌΠΈ Π² ΠΌΠ΅Π»ΠΊΠΈΡ
ΠΈΠ½ΡΡΠ°ΠΌΠΈΠΎΠΊΠ°ΡΠ΄ΠΈΠ°Π»ΡΠ½ΡΡ
ΡΠΎΡΡΠ΄Π°Ρ
. ΠΠΈΠΌΡΠΎΡΠΈΡΠ°ΡΠ½ΡΠΉ ΠΌΠΈΠΎΠΊΠ°ΡΠ΄ΠΈΡ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ Π² 1 % Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠΉ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΠ΅ ΠΊΠ°ΡΠ΄ΠΈΠΎΠΌΠΈΠΎΡΠΈΡΠΎΠ² ΠΏΡΠΈ COVID-19 ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΌΡΠ»ΡΡΠΈΡΠ°ΠΊΡΠΎΡΠ½ΡΠΌ ΠΈ ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡΠΌΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ ΡΠ΅ΡΠ΄Π΅ΡΠ½ΠΎ-ΡΠΎΡΡΠ΄ΠΈΡΡΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ, ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡΠΌΠΈ ΠΈΠ½ΡΡΠ°ΠΌΠΈΠΎΠΊΠ°ΡΠ΄ΠΈΠ°Π»ΡΠ½ΡΡ
ΡΠΎΡΡΠ΄ΠΎΠ² Π²ΡΠ»Π΅Π΄ΡΡΠ²ΠΈΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ SARS-CoV-2 ΠΈ Π³ΠΈΠΏΠΎΠΊΡΠΈΠ΅ΠΉ, ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΡΡΡΡΠΈΠΌ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΠ΅ΠΌ Π»Π΅Π³ΠΊΠΈΡ
ΠΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΡ ΠΏΡΠ΅Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠ°ΠΏΠ° ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π΄Π»Ρ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π³ΠΈΡΡΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΠΈΠΎΠΏΡΠ°ΡΠΎΠ² ΡΠΊΠ΅Π»Π΅ΡΠ½ΠΎΠΉ ΠΌΡΡΡΡ Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ Π½Π΅ΡΠ²Π½ΠΎ-ΠΌΡΡΠ΅ΡΠ½ΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ
Diagnosis of neuromuscular diseases is complicated by the variety of clinical manifestations and requires the use of additional methods, an importantΒ place among which is the pathomorphological study of skeletal muscle biopsy. Despite the fact that the procedure for taking a muscle biopsyΒ is not technically difficult, to obtain informative material a multitude of conditions must be observed at the stages of pre-analytical processingΒ of the obtained tissue samples. Violation of the technology of taking, storing and fixing the material contributes to the formation of artifacts thatΒ limit the possibilities for further analysis of the morphological changes in tissue biopsy. A comparison was made of the effectiveness of various methodsΒ for cryoprocessing of muscle tissue samples and the manufacture of histological specimens with a subsequent assessment of morphologicalΒ changes. As a result, the main causes of artifacts were identified. The optimal method for processing muscle biopsy specimens is indicated, whichΒ makes it possible to prevent the appearance of artifacts as much as possible and to ensure the preservation of tissue for research.ΠΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° Π½Π΅ΡΠ²Π½ΠΎ-ΠΌΡΡΠ΅ΡΠ½ΡΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ Π·Π°ΡΡΡΠ΄Π½Π΅Π½Π° ΠΌΠ½ΠΎΠ³ΠΎΠΎΠ±ΡΠ°Π·ΠΈΠ΅ΠΌ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΠΉ ΠΈ ΡΡΠ΅Π±ΡΠ΅Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ², Π²Π°ΠΆΠ½ΠΎΠ΅ ΠΌΠ΅ΡΡΠΎ ΡΡΠ΅Π΄ΠΈ ΠΊΠΎΡΠΎΡΡΡ
Π·Π°Π½ΠΈΠΌΠ°Π΅Ρ ΠΏΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π±ΠΈΠΎΠΏΡΠΈΠΈ ΡΠΊΠ΅Π»Π΅ΡΠ½ΠΎΠΉ ΠΌΡΡΡΡ. ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΠΎ ΡΡΠΎ ΠΏΡΠΎΡΠ΅Π΄ΡΡΠ° Π²Π·ΡΡΠΈΡ ΠΌΡΡΠ΅ΡΠ½ΠΎΠΉ Π±ΠΈΠΎΠΏΡΠΈΠΈ ΡΠ΅Ρ
Π½ΠΈΡΠ΅ΡΠΊΠΈ Π½Π΅ ΡΠ»ΠΎΠΆΠ½Π°, Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° ΡΡΠ΅Π±ΡΠ΅ΡΡΡ ΡΠΎΠ±Π»ΡΠ΄Π΅Π½ΠΈΠ΅ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π° ΡΡΠ»ΠΎΠ²ΠΈΠΉ Π½Π° ΡΡΠ°ΠΏΠ°Ρ
ΠΏΡΠ΅Π°Π½Π°Π»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΡΠΊΠ°Π½ΠΈ. ΠΠ°ΡΡΡΠ΅Π½ΠΈΠ΅ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈΒ Π²Π·ΡΡΠΈΡ, Ρ
ΡΠ°Π½Π΅Π½ΠΈΡ ΠΈ ΡΠΈΠΊΡΠ°ΡΠΈΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π°ΡΡΠ΅ΡΠ°ΠΊΡΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΠΎΠ³ΡΠ°Π½ΠΈΡΠΈΠ²Π°ΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅Π³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΡΠΊΠ°Π½ΠΈ Π±ΠΈΠΎΠΏΡΠ°ΡΠ°. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΊΡΠΈΠΎΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΌΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΈ ΠΈΠ·Π³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΈΡ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΉ ΠΎΡΠ΅Π½ΠΊΠΎΠΉ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ.Β Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π±ΡΠ»ΠΈ Π²ΡΡΠ²Π»Π΅Π½Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΡΠΈΡΠΈΠ½Ρ Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΡ Π°ΡΡΠ΅ΡΠ°ΠΊΡΠΎΠ². ΠΠ±ΠΎΠ·Π½Π°ΡΠ΅Π½ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΌΡΡΠ΅ΡΠ½ΡΡ
Β Π±ΠΈΠΎΠΏΡΠ°ΡΠΎΠ², ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠΈΠΉ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎ ΠΏΡΠ΅Π΄ΠΎΡΠ²ΡΠ°ΡΠΈΡΡ ΠΏΠΎΡΠ²Π»Π΅Π½ΠΈΠ΅ Π°ΡΡΠ΅ΡΠ°ΠΊΡΠΎΠ² ΠΈ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΡΡ ΡΠΎΡ
ΡΠ°Π½Π½ΠΎΡΡΡ ΡΠΊΠ°Π½ΠΈ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
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