Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining

A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light. The results show that antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed

Structural behavior of reinforced concrete slab rigid frame bridge with h-shaped steels

The authors proposed a reinforced concrete slab bridge with H-shaped steels which has high load-carrying capacity in order to reduce the construction cost. Our past study clarified that H-shape steels without stud dowel and concrete could compose hybrid section and static load was equally distributed each H-shaped steel in elasticity stage. Considering the described above it is possible to design this type of bridge as composite. Moreover it clarified that the cyclically loaded this type bridge has the high fatigue capacity and load-carrying capacity as same as the statically (non- cyclically) loaded that bridge. As next step the authors proposed a reinforced concrete slab rigid frame bridge with H-shaped steels in order to reduce the maintenance cost and to increase seismic performance. Especially the rigid connection method of H-shaped steel and re-bar in the abutments was proposed. Then the static loading test was carried out to grasp load-carrying capacity. As the results of the experiment destructive behavior of the reinforced concrete slab rigid frame bridge with H-shaped steels was clarified and also the validity of the connection method was clarified