128 research outputs found

    Insitu Remediation of Mercury Contaminated Sites

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    Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

    Video Mining using LIM Based Clustering and Self Organizing Maps

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    AbstractVideo mining has grown as an energetic research area and given incremental concentration in recent years due to impressive and rapid raise in the volume of digital video databases. The aim of this research work is to find out new objects in videos. This work proposes a novel approach for video mining using LIM based clustering technique and self organizing maps to recognize novelty in the frames of video sequence. The proposed work is designed and implemented on MATLAB. It is tested with the sample videos and provides promising results. And it is suitable for day to day video mining applications and object detection systems including remote video surveillance in defense for national and international border tracking

    IN VITRO EVALUATION OF CYTOTOXICITY, OXIDATIVE STRESS, DNA DAMAGE AND INFLAMMATION INDUCED BY DIESEL EXHAUST PARTICLES IN HUMAN A549 LUNG CELLS AND MURINE RAW 264.7 MACROPHAGES

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    Objective: The aim of the present study was to examine the in vitro oxidative stress, cytotoxicity, inflammation and DNA damage induced by Diesel Exhaust Particles (DEPs). Methods: Alveolar macrophages (murine RAW 264.7 cells) and cultured type II epithelium cells (human A549 lung cells) were exposed to various concentrations of Diesel Exhaust Particles for 24 h. The experiment was evaluated for cell viability, oxidative stress, cytotoxicity, inflammation and DNA damage parameters. Results: The results showed that overall both cell lines had similar patterns in response to the oxidative stress, cytotoxicity, inflammation and DNA damage parameters induced by DEPs. Vehicle control showed no changes compared to the control. Both cells showed significant changes at the dose of 20 μg/mL and significant changes were found in cytotoxicity, oxidative stress, DNA damage and inflammation indexes. Conclusion: Hence, exposure to DEPs resulted in doseâ€dependent toxicity in cultured A549 cells and RAW264.7cells and was closely correlated to increased inflammation and oxidative stress

    Tracing Hidden Herbivores: Time-Resolved Non-Invasive Analysis of Belowground Volatiles by Proton-Transfer-Reaction Mass Spectrometry (PTR-MS)

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    Root herbivores are notoriously difficult to study, as they feed hidden in the soil. However, root herbivores may be traced by analyzing specific volatile organic compounds (VOCs) that are produced by damaged roots. These VOCs not only support parasitoids in the localization of their host, but also may help scientists study belowground plant-herbivore interactions. Herbivore-induced VOCs are usually analyzed by gas-chromatography mass spectrometry (GC-MS), but with this off-line method, the gases of interest need to be preconcentrated, and destructive sampling is required to assess the level of damage to the roots. In contrast to this, proton-transfer-reaction mass spectrometry (PTR-MS) is a very sensitive on-line, non-invasive method. PTR-MS already has been successfully applied to analyze VOCs produced by aboveground (infested) plant parts. In this review, we provide a brief overview of PTR-MS and illustrate how this technology can be applied to detect specific root-herbivore induced VOCs from Brassica plants. We also specify the advantages and disadvantages of PTR-MS analyses and new technological developments to overcome their limitations

    Role of biology in the air-sea carbon flux in the Bay of Bengal and Arabian Sea

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    A physical-biological-chemical model (PBCM) is used for investigating the seasonal cycle of air-sea carbon flux and for assessing the effect of the biological processes on seasonal time scale in the Arabian Sea (AS) and Bay of Bengal (BoB), where the surface waters are subjected to contrasting physical conditions. The formulation of PBCM is given in Swathi et al (2000), and evaluation of several ammonium-inhibited nitrate uptake models is given in Sharada et al (2005). The PBCM is here first evaluated against JGOFS data on surface pCO2 in AS, Bay of Bengal Process Studies (BoBPS) data on column integrated primary productivity in BoB, and WOCE Il data on dissolved inorganic carbon (DIC) and alkalinity (ALK) in the upper 500 meters at 9°N in AS and at 10°N in BoB in September-October. There is good qualitative agreement with local quantitative discrepancies. The net effect of biological processes on air-sea carbon flux on seasonal time scale is determined with an auxiliary computational experiment, called the abiotic run, in which the biological processes are turned off. The difference between the biotic run and abiotic run is interpreted as the net effect of biological processes on the seasonal variability of chemical variables. The net biological effect on air-sea carbon flux is found to be highest in southwest monsoon season in the northwest AS, where strong upwelling drives intense new production. The biological effect is larger in AS than in BoB, as seasonal upwelling and mixing are strong in AS, especially in the northeast, while coastal upwelling and mixing are weak in BoB

    Handwashing Action Detection System for an Autonomous Social Robot

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    Young children are at an increased risk of contracting contagious diseases such as COVID-19 due to improper hand hygiene. An autonomous social agent that observes children while handwashing and encourages good hand washing practices could provide an opportunity for handwashing behavior to become a habit. In this article, we present a human action recognition system, which is part of the vision system of a social robot platform, to assist children in developing a correct handwashing technique. A modified convolution neural network (CNN) architecture with Channel Spatial Attention Bilinear Pooling (CSAB) frame, with a VGG-16 architecture as the backbone is trained and validated on an augmented dataset. The modified architecture generalizes well with an accuracy of 90% for the WHO-prescribed handwashing steps even in an unseen environment. Our findings indicate that the approach can recognize even subtle hand movements in the video and can be used for gesture detection and classification in social robotics

    Prognostic significance of non-HPV16 genotypes in oropharyngeal squamous cell carcinoma

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    Recent studies have found that cases with oropharyngeal squamous cell carcinoma (OPSCC) positive for HPV16 genotype have better overall survival compared with cases positive for other HPV genotypes. We sought to further replicate these studies and determine if this relationship is modified by expression of p16 tumor suppressor protein

    A Blueprint to Address Research Gaps in the Development of Biomarkers for Pediatric Tuberculosis

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    Childhood tuberculosis contributes significantly to the global tuberculosis disease burden but remains challenging to diagnose due to inadequate methods of pathogen detection in paucibacillary pediatric samples and lack of a child-specific host biomarker to identify disease. Accurately diagnosing tuberculosis in children is required to improve case detection, surveillance, healthcare delivery, and effective advocacy. In May 2014, the National Institutes of Health convened a workshop including researchers in the field to delineate priorities to address this research gap. This blueprint describes the consensus from the workshop, identifies critical research steps to advance this field, and aims to catalyze efforts toward harmonization and collaboration in this are

    Assessment of the Linkages and Leakages in a Cloud-Based Computing Collaboration among Construction Stakeholders

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    The uniqueness of the construction industry can be understood in the large volumes of information in terms of paper works, processes, and communication disseminated daily. The voluminous information requires close coordination which cloud based systems offer. The aim of this research is to assess the linkages and leakages in a cloud-based computing collaboration among construction stakeholders. A purposive sampling technique was used in selecting the participants of the study. A questionnaire based instrument was distributed to eighty (80) construction stakeholder in Lagos State, Nigeria. The data collected was analyzed using SPSS v.21. Statistical tools such as frequencies, stacked bars, mean scores, factor analysis, and analysis of variance (ANOVA) were used in the study. The result of the study revealed that construction stakeholders rarely utilize cloud-based technologies for their construction processes. Construction professionals that access cloud based technologies, do so with their smart mobile phones, laptops, and tablet. The study classified the leakages that exist in the use of cloud-based technologies as securitybased factors, cloud-based infrastructure factors, and cloud-based benefits deficiency based factors. In conclusion, the study revealed that the linkages in the use of cloudbased technologies include knowledge sharing, remote access of back-office activities and engendering collaboration among construction stakeholders. It was recommended that construction stakeholders should leverage on the benefits cloud-based technologies has to offer in today’s competitive economy. Construction stakeholdersshould be adequately informed on the available cloud-based computing technologies and the additions it can bring into the construction process. Cloud computing technology vendors should improve on the security and privacy features of the platform for adequate protection of building data

    \u3cem\u3eStreptococcus agalactiae \u3c/em\u3eStrains with Chromosomal Deletions Evade Detection with Molecular Methods

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    Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culture-positive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample wa
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