Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deepwater rice (Oryza sativa L.) cultivars of Bangladesh

Experiments were carried out to induce embryogenic callus and plant regeneration from five different deepwater rice cultivars. Using mature embryos as explant, all the cultivars demonstrated high callusinduction and plant regeneration frequencies. When coleoptile and root segments used as explants, they had low frequencies of embryogenesis. Depending on different genotypes, the best plant regeneration was obtained on LS (Linsmaier and Skoog, 1965) based medium supplemented with 2 mgl-1 BAP + 1.5 mg l-1 2,4-D. Large variabilities in callus growth and plant regeneration potential were revealed among the cultivars tested. Cultivar HA-8 formed a high frequency (78%) of callus than that of other cultivars. In contrast cv. HA-1 produced the highest percentage (72%) of plant regeneration. The callus growth potential was not correlated with the plant regeneration potential. Coleoptiles and root segments produced calli, which did not develop any shoot bud in regeneration media. Moreover, thecalli turned blackish, watery and translucent after 25-28 days of culture. It is clear that mature seed scutellum (MSS) is the best explant for callus induction and plant regeneratio

Proceedings of International Conference on Emerging Trends in Engineering and Advanced Science

This conference proceedings contains articles on the various research ideas of the academic community and practitioners presented at the International Conference on Emerging Trends in Engineering and Advanced Science (ICETEAS-2021). ICETEAS-2021 was organized by the Department of Electrical and Electronic Engineering & Department of Mechatronics Engineering, World University of Bangladesh, Dhaka, Bangladesh on July 4th-5th November 2021. Conference Title: International Conference on Emerging Trends in Engineering and Advanced ScienceConference Acronym: ICETEAS-2021Conference Date: 4-5 November 2021Conference Location: Online (Virtual Mode)Conference Organizers: World University of Bangladesh, Dhaka, Bangladesh