9 research outputs found
ΠΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌ Π²ΡΡΠ°Π²ΠΊΠΈ/Π΄Π΅Π»Π΅ΡΠΈΠΈ Π³Π΅Π½Π° ΠΠΠ€ ΡΠ²ΡΠ·Π°Π½ Ρ Π³Π»ΠΈΠΎΠ±Π»Π°ΡΡΠΎΠΌΠΎΠΉ Ρ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΡΠ°Π½Π°: ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ»ΡΡΠ°ΠΉ-ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ
Background. The insertion/deletion (I/D) polymorphism of the angiotensin-converting enzyme (ACE) gene has recently been reported to be associated with the pathogenesis and development of human cancers.This study aimed to assess the potential association between ACE (I/D) polymorphism and glioblastoma in an Iranian population.Material and Methods. This case-control study was conducted on 80 patients with glioblastoma and 80 healthy blood donors as controls. Gap-polymerase chain reaction (Gap-PCR) was used to determine the ACE (I/D) genotypes. PCR products were separated and measured by electrophoresis on a 2 % agarose gel.Results. Analysis of demographic data showed a significant difference in the family history of cancer between the case and control groups (p=0.03). The distribution of ACE gene variants including II, ID, and DD genotypes was also calculated, and significant differences were seen in the DD genotype (p=0.03) and D allele (p=0.04) between the glioblastoma cases and controls.Conclusion. ACE gene polymorphism was associated with glioblastoma in the study population. Further studies are needed to approve this finding.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΠ΅Π΄Π°Π²Π½ΠΎ ΡΠΎΠΎΠ±ΡΠ°Π»ΠΎΡΡ, ΡΡΠΎ ΠΈΠ½ΡΠ΅ΡΡΠΈΠΎΠ½Π½ΠΎ-Π΄Π΅Π»Π΅ΡΠΈΠΎΠ½Π½ΡΠΉ (I/D) ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌ Π³Π΅Π½Π° Π°Π½Π³ΠΈΠΎΡΠ΅Π½Π·ΠΈΠ½-ΠΏΡΠ΅Π²ΡΠ°ΡΠ°ΡΡΠ΅Π³ΠΎ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° (ΠΠΠ€) ΡΠ²ΡΠ·Π°Π½ Ρ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅Π·ΠΎΠΌ ΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ΠΌ ΡΠ°ΠΊΠ° ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°.Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»Π° ΠΎΡΠ΅Π½ΠΊΠ° ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ I/D ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠΌ Π³Π΅Π½Π° ΠΠΠ€ ΠΈ Π³Π»ΠΈΠΎΠ±Π»Π°ΡΡΠΎΠΌΠΎΠΉ Ρ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΡΠ°Π½Π°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ»ΡΡΠ°ΠΉ-ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ ΡΡΠ°ΡΡΠ²ΠΎΠ²Π°Π»ΠΈ 80 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π³Π»ΠΈΠΎΠ±Π»Π°ΡΡΠΎΠΌΠΎΠΉ ΠΈ 80 Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄ΠΎΠ½ΠΎΡΠΎΠ² Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π³ΡΡΠΏΠΏΡ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ. ΠΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½Π°Ρ ΡΠ΅ΠΏΠ½Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ (Gap-PCR) ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»Π°ΡΡ Π΄Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π³Π΅Π½ΠΎΡΠΈΠΏΠΎΠ² I/D ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ° Π³Π΅Π½Π° AΠΠ€. ΠΠ¦Π -ΠΏΡΠΎΠ΄ΡΠΊΡΡ ΡΠ°Π·Π΄Π΅Π»ΡΠ»ΠΈ ΠΈ ΠΈΠ·ΠΌΠ΅ΡΡΠ»ΠΈ ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π·ΠΎΠΌ Π² 2 % Π°Π³Π°ΡΠΎΠ·Π½ΠΎΠΌ Π³Π΅Π»Π΅.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ½Π°Π»ΠΈΠ· Π΄Π΅ΠΌΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π΄Π°Π½Π½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°Π» Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΡ ΡΠ°Π·Π½ΠΈΡΡ Π² ΡΠ΅ΠΌΠ΅ΠΉΠ½ΠΎΠΉ ΠΈΡΡΠΎΡΠΈΠΈ ΡΠ°ΠΊΠ° ΠΌΠ΅ΠΆΠ΄Ρ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ (p=0,03). ΠΡΠ»ΠΎ ΡΠ°ΡΡΡΠΈΡΠ°Π½ΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² Π³Π΅Π½Π° ΠΠΠ€, Π²ΠΊΠ»ΡΡΠ°Ρ Π³Π΅Π½ΠΎΡΠΈΠΏΡ II, ID ΠΈ DD, ΠΈ Π±ΡΠ»ΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ Π² Π³Π΅Π½ΠΎΡΠΈΠΏΠ΅ DD (p=0,03) ΠΈ Π°Π»Π»Π΅Π»Π΅ D (p=0,04) ΠΌΠ΅ΠΆΠ΄Ρ Π³ΡΡΠΏΠΏΠΎΠΉ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ Π³Π»ΠΈΠΎΠ±Π»Π°ΡΡΠΎΠΌΠΎΠΉ ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌ Π³Π΅Π½Π° AΠΠ€ Π±ΡΠ» ΡΠ²ΡΠ·Π°Π½ Ρ Π³Π»ΠΈΠΎΠ±Π»Π°ΡΡΠΎΠΌΠΎΠΉ Π² ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠΉ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ. ΠΠ΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΡ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ, ΡΡΠΎΠ±Ρ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠ΄ΠΈΡΡ ΡΡΠΈ Π΄Π°Π½Π½ΡΠ΅
A r c h i v e o f S I D Iranian Chemical Society Molecular Cloning, Expression and Sequence Analysis of DNA Polymerase I from an Iranian Thermophilic Bacterium, Bacillus sp. G (2006)
Thermostable DNA polymerases are widely used in DNA amplification reactions such as the Polymerase Chain Reaction (PCR), requiring the activity of the enzymes at high temperatures. The aim of the present study was to assess the potential biotechnological capabilities of Iranian thermostable DNA polymerases. To this end, we cloned the gene encoding a DNA polymerase from a novel thermophilic eubacterium, Bacillus sp. G (2006). Phylogentic analysis of this gene revealed that the new isolate belongs to the genera Bacillus. Sequence analysis of the fragment produced by degenerate primers also showed that it consists of 2,631 bp encoding an 876 amino acid protein, and subsequent amino acid sequence analysis of this DNA polymerase showed that it belongs to family A-type DNA polymerases. The expression vector pET28a (+) was chosen for expression of the gene fragment in the mesophilic host bacterium E. coli BL21. This expression vector has some advantages such as attachment of a Poly-His tag to the N-terminus of the protein for the ease of purification and a powerful promoter of lac-Z induced by IPTG. The band corresponding to the protein product was observed in the molecular weight range of about 100KDa on the SDS-PAGE gel after heat and Ni +2 -NTA column chromatography. Using the dot blot technique, the polymerase activity of the enzyme was qualitatively confirmed at 70 Β°C. Therefore, it is suggested that optimizations of this activity could make this enzyme appropriate for PCR processes in future