Effect of Genotype, Gender, and Feed Restriction on Slaughter Results and Meat Quality of Broiler Chickens

Slaughter results and meat quality were evaluated in 768 broilers according to genotype (standard breast yield vs. high breast yield), gender, and feeding systems (ad libitum vs. feed restriction from 13 to 21 d of age). Standard-yield chickens had higher carcass weights (2358 g vs. 2319 g; P < 0.001) and hind legs proportion (31.1% vs. 30.6%; P < 0.01), and lower dressing out percentage (73.6% vs. 74.0%; P < 0.01) compared to high-yield birds, besides lower meat L* index (45.3 vs. 46.2; P < 0.05), higher final pH (5.89 vs. 5.85; P < 0.05) and thawing losses (10.5% vs. 9.43%; P < 0.05). Males showed higher carcass weight (+24%), dressing percentage (+0.7%), and hind leg yield (+4%) (P < 0.001) than females. Restricted birds had lower carcass weight (\u20132%; P < 0.001) and dressing percentage (-0.3%) (P < 0.05) than those always fed ad libitum. As what concerns meat quality, gender and feeding system aff ected only meat final pH, lower in ad libitum group than in restricted one and in females than males. In conclusions, slaughter results and carcass traits changed especially with genotype and gender, coherently with slaughter weight whereas meat quality was mostly affected by genotype

Effects of the feeding system on performance and myopathy occurrence in two broiler chicken genotypes

To evaluate whether performance and myopathy occurrence differed according to genotype (Cobb 500 vs. Ross 308) and feeding system (AL: ad libitum vs. ER: early restricted from 13 to 23 d of age, vs. LR: late restricted from 27 to 37 d; restriction rate: 80% of ad libitum), 828 day- old male chicks were assigned to 6 groups (2 x 3 arrangement), housed in 36 pens, and controlled for: individual live weight (weekly) and pen feed intake (daily) until slaughter (48 d); white striping and wooden breast occurrence at slaughter. Individual data were analysed by PROC MIXED of SAS (fixed effects: feeding system, genotype, and interaction; random effect: pen); pen feed intake and myopathy occurrence were analysed by PROC GLM and CATMOD, respectively. The feeding system affected performance: at the end of the first period (1-22 d), ER chickens showed lower weight gain (40.5 g/d vs. 47.8 g/d and 48.0 g/d), feed intake (50.8 g/d vs 61.0 g/d and 60.2 g/d) and weight (903 g vs 1056 g and 1059 g) than AL and LR broilers (P<0.001); at the end of the second period (23-48 d), ER chickens showed higher weight gain (98.3 g/d vs 93.6 g/d and 90.2 g/d) and feed intake (182 g/d vs. 177 g/d and 171 g/d) compared to AL and LR chickens (P<0.001). Final live weight was the highest in AL group, intermediate in ER group, and the lowest in the LR one (3482 g, 3454 g, and 3399 g; P<0.01). Feed conversion in the whole period did not change with the feeding system. At gross examination, white striping occurrence changed from 77.8% to 67.1%, and 81.7% in AL, ER and LR broilers (p<0.10). Differences between genotypes were evident from the first day and, at the end of the trial, weight gain (74.3 g/d vs 70.1 g/d), feed intake (126 g/d vs 114 g/d), feed conversion (1.69 vs 1.64), and live weight (3548 g vs. 3342 g) were higher in the Ross than in the Cobb chickens (P<0.001). At slaughter, the rate of white-striped breasts was similar (on average 75.5%), but the occurrence of severely white-striped breasts was higher in the Ross than in the Cobb chickens (25.9% vs. 7.41%; P<0.001). Wooden breast occurrence (on average 5.1%) did not change with the feeding system or the genotype. In conclusion, under our conditions, a late feed restriction did not permit to recover performance at the end of the trial nor to control white striping occurrence. Moreover, the genotype affected growth rate and white striping degree: the highest the growth rate, the highest the severity of white striping

Effect of stocking density of fish on water quality and growth performance of European Carp and leafy vegetables in a low-tech aquaponic system

Aquaponics (AP) is a semi-closed system of food production that combines aquaculture and hydroponics and represents a new agricultural system integrating producers and consumers. The aim of this study was to test the effect of stocking densities (APL, 2.5 kg m-3; APH, 4.6 kg m-3) on water quality, growth performance of the European Carp (Cyprinus carpio L.), and yield of leafy vegetables (catalogna, lettuce, and Swiss Chard) in a low-technology AP pilot system compared to a hydroponic cultivation. The AP daily consumption of water due to evapotranspiration was not different among treatments with an average value of 8.2 L d-1, equal to 1.37% of the total water content of the system. Dissolved oxygen was significantly (p < 0.05) different among treatments with the lowest median value recorded with the highest stocking density of fish (5.6 mg L-1) and the highest median value in the hydroponic control (8.7 mg L-1). Marketable yield of the vegetables was significantly different among treatments with the highest production in the hydroponic control for catalogna (1.2 kg m-2) and in the APL treatment for Swiss Chard (5.3 kg m-2). The yield of lettuce did not differ significantly between hydroponic control and APL system (4.0 kg m-2 on average). The lowest production of vegetables was obtained in the APH system. The final weight (515 g vs. 413 g for APL and APH, respectively), specific growth rate (0.79% d-1 vs. 0.68% d-1), and feed conversion (1.55 vs. 1.86) of European Carp decreased when stocking density increased, whereas total yield of biomass was higher in the APH system (4.45 kg m-3 vs. 6.88 kg m-3). A low mortality (3% on average) was observed in both AP treatments. Overall, the results showed that a low initial stocking density at 2.5 kg m-3 improved the production of European Carp and of leafy vegetables by maintaining a better water quality in the tested AP system

Influence of group housing, lactation system and floor type on performance and injuries in rabbit does

To evaluate the effect of group housing, lactation system, and floor type, 40 crossbred multiparous pregnant rabbit does were transferred to individual pens (0.5 m x 1.0 m); 8 does were kept individually during all the trial (I; n=8); the other 32 does were kept in 8 collective pens (2.0 m x 1.0 m, by joining 4 individual pens) in stable groups of 4 individuals (G; n=8) from 8 d until 2 d before kindling and from 2 d until 33 d after kindling. Half pens had wire net floor covered with plastic mats (W; n=8) and the other half plastic slatted floor (P; n=8). Within G does, in half pens (n=4) each doe nursed its own litter (fixed lactation, F); in the remaining half, each doe randomly nursed the litters of the group (random lactation, R). Doe performance at kindling and during lactation, litter performance from standardization until weaning (33 d), and skin injuries on G does (5, 12, 19, 26 and 34 d after kindling) were controlled. The effect of the housing system on performance was tested by PROC GLM of SAS. Within G does, the effect of the lactation system and floor type was evaluated by PROC MIXED (pen as random effect); the injury frequency was analysed by PROC FREQ according to observation day, lactation system, and floor type. Housing system did not affect doe kindling or lactation performance; litter weight at standardization was higher for G than I does (680 vs 619 g, P<0.05), but differences disappeared at weaning. The lactation system showed some residual effects when controlled lactation finished, nests were opened, and kits free of moving. In fact, from 19 to 33 d after kindling, does and litters had higher feed intake (679 g/d vs 602 g/d; P<0.05) and, at 33 d, kits were heavier (775 vs 748 g, P<0.05) in R than in F pens. Floor type (W vs P pens) affected milk production (244 vs 225 g/d; P=0.06), and kit weight at the end of controlled lactation (301 vs 290 g; P<0.05), without residual effects at weaning. The injury frequency changed after the group formation: 34% at 5 d, 47% at 12 d, 13% at 19 and 26 d, and 10% at 34 d of lactation (P<0.05). In conclusion, under our conditions, group housing did not negatively affect doe performance; lactation system and floor type had minor effects; aggressiveness among does was evident after group formation during early lactation but decreased in the late lactation. Further recordings on more reproductive cycles would be necessary to confirm the present results

Effect of dietary supplementation with insect fats on growth performance, digestive efficiency and health of rabbits

Background: The present work aimed at evaluating the effect of the dietary replacement of soybean oil (S) by two types of insect fats extracted from black soldier fly larvae (H, Hermetia illucens L.) and yellow mealworm larvae (T, Tenebrio molitor L.) on growth performance, nutrient digestibility, blood parameters, intestinal morphology and health of growing rabbits. Methods: At weaning, 200 crossbred rabbits (36 days old) were allotted to five dietary treatments (40 rabbits/group): a control diet (C) containing 1.5% of soybean oil and four experimental diets where soybean oil was partially (50%) or totally (100%) substituted by H (H50 and H100) or T (T50 and T100) fats. Total tract digestibility was evaluated on 12 rabbits per treatment. The growth trial lasted 41 d and, at slaughtering (78 days old), blood samples were collected from 15 rabbits per treatment, morphometric analyses were performed on duodenum, jejunum and ileum mucosa, and samples of liver, spleen and kidney were submitted to histological evaluation. Results: No difference was observed between the control and the experimental groups fed insect fats in terms of performance, morbidity, mortality and blood variables. The addition of H and T fats did not influence apparent digestibility coefficients of dry matter, protein, ether extract, fibre fractions and gross energy. Gut morphometric indices and organ histopathology were not affected by dietary inclusion of H and T fats. Conclusions: H and T fats are suitable sources of lipid in rabbit diets to replace soybean oil without any detrimental effect on growth performance, apparent digestibility, gut mucosa traits and health

Evaluation of carcass and meat traits of Muscovy duck fed with black soldier fly partially defatted meal

The aim of this study was to evaluate the carcass characteristics and breast meat quality in Muscovy duck (Cairina moschata domestica) fed different inclusion levels of a partially defatted black soldier fly larva (BSF) meal. A total of 256 Muscovy ducklings (average live weight, LW: 71.32\ub12.70 g) were reared from day 3 to day 48 and randomly allotted in 32 pens (8 replicates/treatment). Four different diets were formulated with increasing substitution level of corn gluten meal with BSF larva meal (0, 3, 6 and 9%; BSF0, BSF3, BSF6 and BSF9, respectively) and divided in 3 feeding phases: starter (1-14 days), grower (14-35 days) and finisher (35-48 days). At day 48, 2 animals/replicate were slaughtered and dissected to determine their carcass yields. The weights of spleen, bursa of Fabricius, liver, heart and abdominal fat were recorded. Breast and thigh muscles were then excised from 16 ducks/treatment and weighted. Ultimate pH (pHu) and L*, a*, b* colour values were then measured on breast muscle. The collected data were tested by means of oneway ANOVA evaluating the effect of dietary BSF inclusion level by polynomial contrasts. Significance was declared at P<0.05. The inclusion of BSF did not affect final LW (2,515.68\ub192.42 g on average). Hot and cold carcass weights showed a quadratic response (P<0.05) to increasing BSF larva meal, with a minimum corresponding to BSF6; however, refrigeration losses were not affected by treatments. Weight of spleen, bursa of Fabricius, liver and heart did not differ among treatments. The weight of abdominal fat showed a quadratic response to increasing BSF meal with a minimum corresponding to BSF6 group (P<0.05). Breast and thigh yields, pHu and L*, a*, b* colour values did not differ among groups. With the exception of BSF6, the inclusion of BSF meal did not affect meat traits and carcass characteristics, confirming the potential use of BSF meal in Muscovy duck diets

Performance, health and slaughter traits of group-housed rabbits submitted to different time-based feed restriction programs

Growth performance, health and slaughter traits were measured in 320 crossbreed rabbits housed in 40 open-top pens (8 rabbits/pen) from weaning to slaughter (34–70 d of age) to evaluate the effects of the different feeding programs: AL, ad libitum feeding; DF, daylight access to feed with fast and complete refeeding; NF, night access to feed with fast and complete refeeding; NS, night access to feed with slow and complete refeeding; NI, night access to feed with very slow and incomplete refeeding. In restricted rabbits, the feeding time decreased from 14 to 9 h/d during the 1st week, remained stable (8 h/d) during the 2nd week, and increased from the beginning of the 3rd week according to three refeeding programs: i) fast until ad libitum (+4 h/d until 24 h/d); ii) slow until ad libitum (+1 h/d until 24 h/d); iii) very slow and still restricted until the end of fattening (+30 min/d until 12 h/d). In the restricted rabbits, feed intake (-3.7%) and feed conversion (–5.6%) were lower compared to AL ones (P<0.001) with the minimum values in the NI group. Diet digestibility increased in the restricted groups compared to AL and in the NI group compared to other restricted groups. Mortality was lower in AL rabbits compared to restricted ones (1.6% vs. 9.0% on average; P=0.04), whereas morbidity and health risk index did not change. Slaughter weight (2608 g), dressing percentage (60.2%), and carcass muscularity were not affected by the feeding program. In conclusion, the NI program represented the best strategy to optimize feed efficiency without impairing growth, slaughter results and carcass traits in growing rabbits. The tested feed restriction programs did not succeed in enhancing rabbit health

Apparent digestibility of insect protein meals for rainbow trout

Insect meals are considered to be promising future ingredients for aquaculture feeds. In past feeding trials in rainbow trout, insect meals were included in diets only on the basis of their nutrients content and energy density without taking into account their biological availability due to the lack of their digestible values. Apparent digestibility (ADC) provides good indication of the bioavailability of nutrients and energy thus providing rational basis for the correct inclusion of feedstuffs. The aim of this research was to assess, in an in vivo trial on rainbow trout, the ADC of five full fat insect meals: one Tenebrio molitor (TM), two Hermetia illucens obtained through two different process (HI1 and HI2), one Musca domestica (MD), and one Alphitobius diaperinus (AD). Fish were fed a high-quality reference diet (R) and test diets obtained mixing the R diet with each of the test ingredients at a ratio of 70:30. Diets contained 1% celite as inert marker. Fish were fed to visual satiety twice a day and faecal samples collected using a continuous automatic device. Faeces were freeze dried and frozen (-20 \ub0C) until analyses. The ADC of dry matter, crude protein and ether extract of each insect meal diet were calculated. ADC for dry matter varied between 70.07 (HI1) and 80.85 (TM). ADC for protein was above 84% in all treatments and resulted the highest in MD, TM and AD treatments. Ether extract apparent digestibility significantly differed among diets with the highest value reported for TM treatment. All treatments reported values higher than 96%. Observed differences could be due to the insect species and meal treatment but in general, tested insect meals were highly digestible for rainbow trout. The results from this research could be useful to optimize the diet formulation

Poly-chlorinated biphenyls (PCB) in European sea bass from different rearing systems

The chemical composition and the level of seven indicator congeners of PCB (BZ/IUPAC no. 28, 52, 101, 118, 138, 153, and 180) were determined in 133 specimens of farm-raised European sea bass (Dicentrarchus labrax). The fish were caught from different aquaculture rearing systems: extensive fish valley, semi-intensive ponds, sea-cages, and intensive concrete tanks. Fresh fillet chemical composition differed among the rearing systems (fat: 2.9, 7.5, 7.1, and 9.4%; P<0.001). Total concentrations of indicator congeners were below the EU limit (200ng/g fat) for meat, poultry and eggs, being the lowest in extensively-reared sea bass (75ng/g fat), intermediate in sea bass from semi-intensive ponds (119) and sea cages (116), and the highest in intensively-reared fish (133) (P<0.001). Similarly, PCB concentrations in fresh fillets were 2,438, 10,116, 8,491, and 12,952pg/g in the four systems (P<0.001). The congener 153 was the most represented in all rearing systems. TEQ concentrations for the dioxin-like congener no. 118 were 50 to 200 times lower than the maximum admitted value. Total concentration of indicator congeners of PCB was poorly correlated with fish slaughter weight (R2=0.17), while highly correlated with fat concentration of fish (R2=0.75)