Clinical review: Biomarkers of acute kidney injury: where are we now?

The recognition that acute kidney injury (AKI) is a significant independent risk factor for morbidity and mortality has resulted in a substantial number of publications over the past 5 years or more. In no small part these have, to a degree, highlighted the inadequacy of conventional markers of renal insufficiency in the acute setting. Much effort has been invested in the identification of early, specific AKI markers in order to aid early diagnosis of AKI and hopefully improve outcome. The search for a 'biomarker' of AKI has seen early promise replaced by a degree of pessimism due to the lack of a clear candidate molecule and variability of results. We outline the major studies described to date as well as discuss potential reasons for the discrepancies observed and suggest that evolution of the field may result in success with ultimately an improvement in patient outcomes

Epidemiology and burden of nasal congestion

Nasal congestion, which may be described as fullness, obstruction, reduced airflow, or being "stuffed up," is a commonly encountered symptom in clinical practice. Systematic study of congestion has largely considered it as a component of a disease state. Conditions associated with congestion include nasal polyposis, obstructive sleep apnea, and anatomic variation; however, most information on the burden of congestion comes from studies of allergic rhinitis and rhinosinusitis, diseases of which congestion is the major symptom. Congestion can be caused by other rhinologic conditions, such as non-allergic rhinitis, viral or bacterial rhinitis, and vasomotor rhinitis. Allergic rhinitis affects as much as one quarter of the population worldwide and imposes a significant economic burden. Additionally, allergic rhinitis significantly impairs quality of life; congestion causes allergic rhinitis sufferers decreased daytime productivity at work or school and reduces night-time sleep time and quality. Annually, rhinosinusitis affects tens of millions of Americans and leads to approximately $6 billion in overall health care expenditures; it has been found to be one of the most costly physical conditions for US employers. Given the high prevalence and significant social and economic burden of nasal congestion, this symptom should be a key consideration in treating patients with rhinologic disease, and there continues to be a significant unmet medical need for effective treatment options for this condition. © 2010 Stewart et al

Cascade atom in high-Q cavity: The spectrum for non-Markovian decay

The spontaneous emission spectrum for a three level cascade configuration atom in a single mode high-Q cavity coupled to a zero temperature reservoir of continuum external modes is determined from the atom-cavity mode master equation using the quantum regression theorem. Initially the atom is in its upper state and the cavity mode empty of photons. Following Glauber, the spectrum is defined via the response of a detector atom. Spectra are calculated for the detector located inside the cavity (case A), outside the cavity end mirror (Case B-end emission), or placed for emission out the side of the cavity (Case C). The spectra for case A and case B are found to be essentially the same. In all the cases the predicted lineshapes are free of instrumental effects and only due to cavity decay. Spectra are presented for intermediate and strong coupling regime situations (where both atomic transitions are resonant with the cavity frequency), for cases of non-zero cavity detuning, and for cases where the two atomic transition frequencies differ. The spectral features for Cases B(A) and C are qualitatively similar, with six spectral peaks for resonance cases and eight for detuned cases. These general features of the spectra can be understood via the dressed atom model. However, Case B and C spectra differ in detail, with the latter exhibiting a deep spectral hole at the cavity frequency due to quantum interference effects.Comment: 29 pages, 14 figures; v2: very minor correction to two equations, thicker lines in some figure

The Role of Consciousness in Memory

Conscious events interact with memory systems in learning, rehearsal and retrieval (Ebbinghaus 1885/1964; Tulving 1985). Here we present hypotheses that arise from the IDA computional model (Franklin, Kelemen and McCauley 1998; Franklin 2001b) of global workspace theory (Baars 1988, 2002). Our primary tool for this exploration is a flexible cognitive cycle employed by the IDA computational model and hypothesized to be a basic element of human cognitive processing. Since cognitive cycles are hypothesized to occur five to ten times a second and include interaction between conscious contents and several of the memory systems, they provide the means for an exceptionally fine-grained analysis of various cognitive tasks. We apply this tool to the small effect size of subliminal learning compared to supraliminal learning, to process dissociation, to implicit learning, to recognition vs. recall, and to the availability heuristic in recall. The IDA model elucidates the role of consciousness in the updating of perceptual memory, transient episodic memory, and procedural memory. In most cases, memory is hypothesized to interact with conscious events for its normal functioning. The methodology of the paper is unusual in that the hypotheses and explanations presented are derived from an empirically based, but broad and qualitative computational model of human cognition

The disappearing women: North West ICT project final report

Project Context The Disappearing Women: Northwest ICT project was embarked upon to further understand why more women leave the sector than are being recruited, 36% of new ICT recruits in the UK (in the first quarter of 2002) were women, yet in the same period, women accounted for 46% of all leavers or ‘disappearing’ women (The DTI Women in IT Champions report 2003, Grey and Healy 2004). This continuing trend shows a decline from 27% of women making up the ICT workforce in 1997 with a drop to 21% in 2004 (The DTI Women in IT Industry report 2005b). The number of women in the ICT sector remains disappointingly small considering that women make up around 50% of the total UK workforce and significantly this figure has gradually continued to fall despite numerous initiatives to attract more women into the sector (see Griffiths and Moore 2006 for a list of high profile ‘women in ICT’ initiatives). The research team’s first gender research project - Women in IT (WINIT) - ran for two years from January 2004 until March 2006 at the Information Systems Institute at The University of Salford and dealt solely with women who worked in the ICT sector in England. The WINIT Project via an online questionnaire and in-depth interviews gathered the stories and experiences of up to 500 participants and 19 interviewees respectively. The project enabled these women’s voices to be heard but the research team were constantly aware that a certain part of the female ICT workforce - the ‘disappearing’ women who had left ICT vowing never to return - had been overlooked and effectively silenced. It was these women who once found, may be able to facilitate a more in-depth understanding of why women were leaving the ICT sector. Having amassed skills and expertise, qualifications in ICT and crossed ICT recruitment barriers (DTI 2005a) the ‘disappearing’ women for whatever reasons decided to change their career trajectories and leave the sector. What ‘chilly’ (Falkner 2004) workplaces, disinterested organisational cultures and indifferent working conditions had these women encountered that became determining factors in leaving the ICT sector? There has been little (if any) research conducted involving this specific cohort of women and The Disappearing Women: North West ICT (DW: NW ICT) project seeks to make a research contribution to what is a continuing statistical and symbolic under-representation of women in the ICT labour market. The DW: NW ICT project was partly funded by the European Social Fund (ESF) from April 2006 until December 2006 under ESF Objective 3, Policy Field 5.1: Improving the Participation of Women. The DW: NW ICT project contributes research to priority 5 and its strategic objective to reduce the level of disadvantage faced by women in the labour market. The project was run in the Information Systems Group, Salford Business School of The University of Salford, Greater Manchester, UK. The report is structured as follows. The first section presents the backdrop for the research, looking in general at women in the ICT labour market in England and then women leaving the ICT sector focussing on the North West of England and more explicitly women leaving ICT employment in the North West of England. The research aims of the project form the following section; they have been loosely classified in to two groupings, the push and pull factors that are contributing to the high attrition rate of women leaving ICT. The methodology follows with the route taken in how this ‘hard to reach’ target sample were finally located, once contacted the life history interview process and procedures adopted is explained in full. The vignettes of the ‘disappearing’ women are included to allow the reader an opportunity to ‘get to know’ these women a little more closely. Key themes that have naturally emerged throughout the interview data analysis process are presented, including hostilities in the ICT workplace, significant events and the process of leaving ICT workplaces and finally stories of the ‘appearing’ women and their current situations are heard. A discussion regarding the findings of the DW: NW ICT project concludes this report

Studies on exploiting semiochemicals for pest management in organic farming systems OF0188

This study addresses the extent to which pest management systems can exploit semiochemicals (defined below) in ways acceptable to organic farming, and determines where the science base needs to be expanded to accommodate specific problems arising in an increasing organic farming sector. It considers whether current knowledge of semiochemical release from particular crop plants, herbs and wild plant species could be investigated further in relation to organic farming practice and identifies how strategies of multiple cropping, that exploit known semiochemical interactions, could be applied to key pest problems in organic production. Where such exploitation is not feasible, other strategies using semiochemicals including traps, extracts of natural products and nature-identical synthetic products are considered. Finally, general and specific directions in which research and development could facilitate greater penetration of the use of semiochemicals in crop protection for organic farming are identified. Semiochemicals are natural products that, by acting as signals, regulate interactions between organisms e.g. plants and insects. Once the semiochemical interactions between a pest and its host plant have been elucidated they can be exploited to regulate the pest population, providing an alternative control strategy to conventional toxicants. The choice of approach by which the semiochemicals are deployed relates to three options, i.e. from a natural plant source, from an extract or as a nature identical synthetic product. However, even where the most natural situations of mixed cropping are used, the scientific basis of the interaction must be established for robustness and sustainability of the approach. A complete understanding of the process allows a risk assessment to be made of any problems that might ensue when exploiting natural systems in different configurations from those encountered naturally. A major approach to using semiochemical based pest control is to exploit ways of repelling pests from crop plants and attracting them towards trap plantations. Deploying semiochemicals generated naturally by plants is consistent with organic farming practice, where a range of mixed cropping techniques are employed already, which ‘unconsciously’ utilise semiochemical effects. Thus, the acceptance and use of systems exploiting aspects of semiochemical deployment demonstrate an emerging role in organic farming practices. However, as emphasised before, a comprehensive knowledge of the semiochemical interactions that underpin these techniques is vital if they are to be exploited fully. Other pest control approaches compatible with organic farming, such as encouragement of beneficial species and the use of reflective surfaces in mulches, may not involve semiochemical effects, but could be exploited more beneficially by integration with semiochemical practices. Semiochemicals generated naturally by plants can be used to influence beneficial organisms as well as invertebrate pests. For example, plant defence chemicals, induced by pest or pathogen infestation, can affect the behaviour of pests and their natural enemies. Semiochemicals can be employed to maximise the impact of parasitic organisms that attack pest populations, for example in the management of refugia for maintaining and increasing populations of these beneficial organisms. In addition, the approach can be applied against other organisms antagonistic to agriculture besides invertebrate pests, for example in weed control, where signals interfering with weed germination can be exploited. Extracts of natural products provide semiochemicals in a form that is familiar and acceptable to organic farming practice, where plant extracts are already used as toxicants or as semiochemical antifeedants and repellents. However, often the scientific basis for use of these materials is limited, and therefore, exploitation is also limited and can be unreliable. By understanding the composition and the mechanism of activity of semiochemicals, natural product extracts can be improved by selection of the best sources of natural materials and appropriate processes of extraction and formulation. Many natural products, particularly pheromones (semiochemicals acting between members of the same species), can be synthesised as nature-identical and the synthetic forms are often indistinguishable from the natural form. Synthesis can be expensive, but where possible, starting materials should be obtained from natural renewable resources. Nature-identical synthetic pheromones are used widely in parts of the world, either deployed in traps for monitoring, mass trapping and lure and kill strategies or for direct pest control approaches such as mating disruption. In addition, manipulation of beneficial species with pheromones is being investigated and synthetic food-related attractants and oviposition attractants have also been developed for pests where pheromones are not available. Already some nature-identical synthetic semiochemicals have been accepted as compatible with organic farming practice. The registration of many sex and aggregation pheromones has been possible because they are nature-identical and are deployed away from the crop or on crop areas that are not consumed. In most cases, semiochemicals, deployed alone, are not sufficiently robust to control pest populations directly. They are most effective when incorporated into strategies, such as the ‘push-pull’ strategy, that are integrated with other forms of pest control, e.g. pathogens, parasitoids and predators, mechanical barriers and resistant plant varieties. The integration of semiochemical approaches with other methods of pest population reduction will help prevent the development of pest resistance to the overall strategy. Since the integrated strategy comprises a number of components that affect different aspects of pest behaviour and development each component can be relatively ineffective when compared to conventional pesticides. However, this has the advantage of not selecting efficiently for resistance to any component of the strategy and thus contributes to the sustainability of the approach. Recommendations 1) Develop a priority list of specific and general problems in organic production to be targeted by semiochemical methodologies in addition to known problems such as in carrot and lettuce production, aphids on a range of vegetable crops and for fruit pests. 2) Develop semiochemical based control methods suitable for 1) and for the targets already known. 3) Provide scientific input, where lacking, for 1 and 2. 4) Encourage greater diversification in organic cropping systems, including agroforestry, so as to exploit current knowledge of semiochemical based control and to pave the way for new interventions as the science develops. 5) Consider semiochemical attributes of non-crop plant inputs including mulches, weeds and multifunctional beneficial plants and the roles that they might play in organic systems. 6) Initiate organic plant breeding programmes, specifically to exploit natural semiochemical release where understood, for crop and companion plants

A reduced-order model of three-dimensional unsteady flow in a cavity based on the resolvent operator

A novel reduced-order model for nonlinear flows is presented. The model arises from a resolvent decomposition in which the nonlinear advection terms of the Navier-Stokes equation are considered as the input to a linear system in Fourier space. Results show that Taylor-G\"ortler-like vortices can be represented from a low-order resolvent decomposition of a nonlinear lid-driven cavity flow. The present approach provides an approximation of the fluctuating velocity given the time-mean and the time history of a single velocity probe

An on-line solid phase extraction procedure for the routine quantification of urinary methylmalonic acid by liquid chromatography-tandem mass spectrometry

Background: The goal of this study was to develop and to validate an improved isotope-dilution-liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of methylmalonic acid (MMA) in urine. Methods: A previously described sample preparation protocol requires two solvent extraction steps, including evaporation. The first extraction is to extract the analyte from the sample, and second occurs following derivatization of the extract. In the method described here, the second evaporation step was substituted by on-line solid phase extraction employing column-switching and a permanent co-polymer based extraction cartridge. A standard validation protocol was applied to investigate the performance of the method. Results: The method was found to be linear in the clinically relevant range of concentrations (6-100 mu mol/L). Total coefficients of variation were below 10% and inaccuracy was <10% for quality control samples at three concentrations. Conclusions: By omitting one evaporation step, the semi-automated method described in this article enables for more convenient work-flow in the quantification of urinary MMA compared to the previous protocol. This is of relevance for MMA measurement in the routine clinical laboratory setting. Validation demonstrated acceptable analytical performance. Clin Chem Lab Med 2010;48:1647-50

Steady streamwise transpiration control in turbulent pipe flow

A study of the the main features of low- and high amplitude steady streamwise wall transpiration applied to pipe flow is presented. The effect of the two transpiration parameters, amplitude and wavenumber, on the flow have been investigated by means of direct numerical simulation at a moderate turbulent Reynolds number. The behaviour of the three identified mechanisms that act in the flow: modification of Reynolds shear stress, steady streaming and generation of non-zero mean streamwise gradients, have been linked to the transpiration parameters. The observed trends have permitted the identification of wall transpiration configurations able to reduce or increase the overall flow rate in -36.1% and 19.3% respectively. A resolvent analysis has been carried out to obtain a description of the reorganization of the flow structures induced by the transpiration

A numerical investigation of the solution of a class of fourth-order eigenvalue problems

This paper is concerned with the accurate numerical approximation of the spectral properties of the biharmonic operator on various domains in two dimensions. A number of analytic results concerning the eigenfunctions of this operator are summarized and their implications for numerical approximation are discussed. In particular, the asymptotic behaviour of the first eigenfunction is studied since it is known that this has an unbounded number of oscillations when approaching certain types of corners on domain boundaries. Recent computational results of Bjorstad & Tjostheim, using a highly accurate spectral Legendre-Galerkin method, have demonstrated that a number of these sign changes may be accurately computed on a square domain provided sufficient care is taken with the numerical method. We demonstrate that similar accuracy is also achieved using an unstructured finite-element solver which may be applied to problems on domains with arbitrary geometries. A number of results obtained from this mixed finite-element approach are then presented for a variety of domains. These include a family of circular sector regions, for which the oscillatory behaviour is studied as a function of the internal angle, and another family of (symmetric and non-convex) domains, for which the parity of the least eigenfunction is investigated. The paper not only verifies existing asymptotic theory, but also allows us to make a new conjecture concerning the eigenfunctions of the biharmonic operator