Effect of various strontium concentrations on its uptake and the content of isoflavonesin soybean sprouts

Metal ions can modify plant metabolism and change the level of biologically active components. In the present study, the impact of short-term exposure to strontium on the accumulation of the metal as well as the content of isoflavones in soybean sprouts was investigated. The seeds were germinated in hydroponics with 0, 1, 1.5, 2.5, 5.0, or 10.0 mM of Sr for 72 hours. The content of strontium was assessed using flame atomic absorption spectrometry and the amount of isoflavones was determined with high performance liquid chromatography. dose-dependent accumulation of Sr and a linear correlation between the Sr concentration in the growth medium and the content of the element in the plant samples were observed. The largest changes in the isoflavone content, compared to the control, were noted in soy sprouts germinated in the presence of 5 and 10 mM of strontium. daidzin, genistin, malonyldaidzin, and malonylgenistin were the dominant isoflavones and their content increased by approx. 28, 44, 34, and 47%, respectively, compared to the control. low amounts of aglycones were found; moreover, their content decreased by ca. 19–30%. Our research can be important for obtaining a natural product enhanced with strontium and isoflavones, which contribute to prevention of osteoporosis associated with endog- enous oestrogen deficit

Oleanolic acid induces migration in Mv1Lu and MDA-MB-231 epithelial cells involving EGF receptor and MAP kinases activation

During wound healing, skin function is restored by the action of several cell types that undergo differentiation, migration, proliferation and/or apoptosis. These dynamics are tightly regulated by the evolution of the extra cellular matrix (ECM) contents along the process. Pharmacologically active flavonoids have shown to exhibit useful physiological properties interesting in pathological states. Among them, oleanolic acid (OA), a pentacyclic triterpene, shows promising properties over wound healing, as increased cell migration in vitro and improved wound resolution in vivo. In this paper, we pursued to disclose the molecular mechanisms underlying those effects, by using an in vitro scratch assay in two epithelial cell lines of different linage: non-malignant mink lung epithelial cells, Mv1Lu; and human breast cancer cells, MDA-MB-231. In every case, we observed that OA clearly enhanced cell migration for in vitro scratch closure. This correlated with the stimulation of molecular pathways related to mitogen-activated protein (MAP) kinases, as ERK1,2 and Jun N-terminal kinase (JNK) 1,2 activation and c-Jun phosphorylation. Moreover, MDA-MB-231 cells treated with OA displayed an altered gene expression profile affecting transcription factor genes (c-JUN) as well as proteins involved in migration and ECM dynamics (PAI1), in line with the development of an epithelial to mesenchymal transition (EMT) status. Strikingly, upon OA treatment, we observed changes in the epidermal growth factor receptor (EGFR) subcellular localization, while interfering with its signalling completely prevented migration effects. This data provides a physiological framework supporting the notion that lipophilic plant extracts used in traditional medicine, might modulate wound healing processes in vivo through its OA contents. The molecular implications of these observations are discussed