ANALYSIS OF TUMOR SPECIFIC PROTEIN EXPRESSION IN GLIOBLASTOMA MULTIFORME (GBMs) TUMORS THROUGH IMMUNOHISTOCHEMISTRY

GBM tumors are the most aggressive and, unfortunately, the most fatal form of brain cancer. GBM tumors with isocitrate dehydrogenase-1 (IDH1) mutation being expressed, lead to higher survival rates in patients that also have full resection of the tumor and chemotherapy. Without this mutation, it is thought that tumors have a higher expression of the protein Basigin and O6-methylguanine-DNA-methyltrasnferase (MGMT) present, causing it to be more aggressive and less responsive to standard care. The objective of this study was to understand the correlation between IDH1 mutation presence and the expression of Basigin and MGMT. The expression of these proteins was observed in tissues sections from GBM tumors. Proteins were labeled with a fluorescent antibody and imaged with a confocal microscope. The tissue images were then analyzed using Imaris software. It was shown that there was a significant difference between the presence of the IDH1 mutation and Basgin, and also MGMT among all of the tissue blocks

Taming the Social Network Jungle: From Web 2.0 to Social Media

The authors explore various Web 2.0, social media as well as communication networks developments. Web 2.0 is defined and viewed as a paradigm shift. They examine how the vast amount and huge variety of information on the Web is attempted to be organized, sorted, ordered, tagged and classified. The nature and role of social networking sites are addressed, especially asking how businesses might find a role here. Moreover, the authors ask how one might reach and connect with Web 2.0 customers and what strategy businesses might pursue. Online shopping communities are explored and opportunities for retailers are identified. Lastly, questions and issues are raised about the implementation of Web 2.0 and the bottom line, i.e. an attempt to see what some businesses are doing in this context and how they embrace Web 2.0 technologies and applications. Conclusions highlighting four predictions for the future of social networking and social media are offered

Long‐term nutrient addition increases respiration and nitrous oxide emissions in a New England salt marsh

© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ecology and Evolution 8 (2018): 4958-4966, doi:10.1002/ece3.3955.Salt marshes may act either as greenhouse gas (GHG) sources or sinks depending on hydrological conditions, vegetation communities, and nutrient availability. In recent decades, eutrophication has emerged as a major driver of change in salt marsh ecosystems. An ongoing fertilization experiment at the Great Sippewissett Marsh (Cape Cod, USA) allows for observation of the results of over four decades of nutrient addition. Here, nutrient enrichment stimulated changes to vegetation communities that, over time, have resulted in increased elevation of the marsh platform. In this study, we measured fluxes of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) in dominant vegetation zones along elevation gradients of chronically fertilized (1,572 kg N ha−1 year−1) and unfertilized (12 kg N ha−1 year−1) experimental plots at Great Sippewissett Marsh. Flux measurements were performed using darkened chambers to focus on community respiration and excluded photosynthetic CO2 uptake. We hypothesized that N‐replete conditions in fertilized plots would result in larger N2O emissions relative to control plots and that higher elevations caused by nutrient enrichment would support increased CO2 and N2O and decreased CH4 emissions due to the potential for more oxygen diffusion into sediment. Patterns of GHG emission supported our hypotheses. Fertilized plots were substantially larger sources of N2O and had higher community respiration rates relative to control plots, due to large emissions of these GHGs at higher elevations. While CH4 emissions displayed a negative relationship with elevation, they were generally small across elevation gradients and nutrient enrichment treatments. Our results demonstrate that at decadal scales, vegetation community shifts and associated elevation changes driven by chronic eutrophication affect GHG emission from salt marshes. Results demonstrate the necessity of long‐term fertilization experiments to understand impacts of eutrophication on ecosystem function and have implications for how chronic eutrophication may impact the role that salt marshes play in sequestering C and N

Short-Term Effects of Thin-Layer Sand Placement on Salt Marsh Grasses: A Marsh Organ Field Experiment

Salt marshes can build in elevation with sea-level rise through accumulation of inorganic sediment and organic matter, but marshes worldwide are under threat of drowning due to rapid rates of sea-level rise that outpace natural marsh building rates. The application of a thin layer of sediment to the marsh surface (thin-layer placement [TLP]) is a tool to build elevation and decrease flooding stress, but its effects on marsh plants are understudied, especially in New England. In a novel application of a marsh organ experiment (i.e. rows of pots at different elevations), the addition of 10 cm of sand to pots planted with Spartina alterniflora and Spartina patens resulted in fewer stems than controls for S. patens but not S. alterniflora after 2 months. However, total biomass and root mass were not significantly impacted for either species, suggesting plants will fully recover from TLP over longer timescales. Effects of TLP on biomass and stem density did not vary significantly by elevation. Although long-term research is still needed, short-term equivalency in biomass between TLP treatments and controls suggests TLP of 10 cm is a promising strategy to enhance the ability of marshes to build vertically as sea level rises in New England

A Multi-Level View of the Antecedents and Consequences of Trust in Virtual Leaders

Although trust is widely acknowledged as critical to virtual teams, little is known regarding the causes and consequences of trust in leaders of virtual teams. This paper examines the antecedents and consequences of trust in virtual team leaders. Using survey and archival data from a massively multiplayer online game (MMOG), this study’s findings show that trust in the leader is affected by team members’ use of synchronous communication and breadth of communication with leaders as well as team members’ distance from each other. Furthermore, reasoning that team size and culture create a shared context qualifying team members’ experiences, we found that team size and collectivistic values diminished the benefits of synchronous communication and breadth of communication, respectively. The findings also revealed that trust in leaders had a positive relationship to team performance. Detailed discussion of the findings is provided in the conclusion of the paper

The Dark Side of Information and Communication Technologies: The View from the Industry-Level of Analysis

The Year 2000 problem spurred companies to rethink investments in information and communication technologies (ICT). Many used the Y2K problem as an opportunity to renew ICT infrastructures, to install integrated enterprise packages, and to pursue new opportunities for ICT-enabled value such as e-commerce, supply chain management, and customer relationship management. Some evidence suggests that these efforts have had substantial payoffs in terms of shareholder value. But can such firm-level benefits persist when competitors catch up or when the success of leaders drives inefficient producers out of business? This panel features NSF-funded researchers whose studies have examined the impacts of ICT at the industry-level of analysis. They show significant industry-level ICT-enabled impacts with potentially negative implications for the firms competing within industries. In the Information Systems field, the ability to gain competitive advantage with ICT has long been an important theme. Although some researchers warned that ICT might contribute to the destruction of competitive advantage, by far the majority of the discourse has centered on how individual firms should invest in ICT. When taking an industry-level view of ICT-enabled competitive advantage, however, we can see its potential dark side. Among the risks ICT poses to the firms in an industry are these: • Fundamentally reducing the cost structure of an industry such that some firms can no longer compete and that others experience squeezed margins • Destruction of in-house competencies (e.g., through radical process change or business process outsourcing) • Investments in ICT are required as a condition of doing business without providing any bottom-line benefits • Increased dependency on external ICT providers leading to business inflexibility and lack of ICT knowledg

MMOGs as Emerging Opportunities for Research on Virtual Organizations and Teams

Massively Multiplayer Online Games (MMOG) offer new promising opportunities to research virtual organizations and teams. The characteristics of MMOGs allow researchers to obtain objective data from a large and multi-national population. Lasting over months or even years, MMOGs facilitate longitudinal studies and ensure a high involvement of participants. Moreover, collecting data from online surveys and game servers keeps the costs of MMOG studies low. In this paper, we illustrate how research in MMOGs can utilize these opportunities to overcome some limitations of traditional research environments. Further we discuss the diverse information and communication technology (ICT) usage in MMOGs and therefore argue that research in MMOGs can provide a glimpse into the future application of ICT in real life organizations

Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma

Introduction The number of aging cancer patients has increased continuously and will do so further in the future. The immune system of elderly people experiences critical changes over the time. Therefore, tumor-induced changes in the immune system are believed to differ in young and elderly cancer patients as well. Methods The effect of aging on the immune system was measured in peripheral blood lymphocytes (PBL) of healthy volunteers (n = 48, 21–84 yrs.) divided into three different age groups. Seventy years was set as a cut-off for defining subjects as elderly. Results were compared to two groups of adult cancer patients, which donated PBL and tumor infiltrating lymphocytes (TIL): young cancer patients (40–69 yrs.; blood: n = 13; TIL: n = 17) and elderly cancer patients (70–90 yrs.; blood: n = 20; TIL: n = 15) with head and neck squamous cell carcinoma (HNSCC). Frequencies and phenotypes of CD4+ and CD8+ T cells as well as regulatory T cells (Treg) were assessed by flow cytometry. Results We observed lower frequencies of CD8+ cytotoxic T cells during aging in both groups. Frequencies of tumor infiltrating regulatory T cells were significantly higher than in the peripheral blood but showed a significant decline in older tumor patients. With increasing age, expression of immunosuppressive CD73 and CCR7 was lower and expression of PD1 elevated on peripheral T cells in healthy volunteers and tumor patients. Conclusion Immunosenescence takes place in healthy donors and cancer patients. Our results suggest that in elderly tumor patients, the immune system is impaired and the tumor-induced immune escape is less pronounced. The increased expression of PD1 implies the potential for effective immunotherapies in elderly, as treatment with checkpoint inhibitors could be more beneficial for elderly HNSCC patients

MSIS 2000: Model Curriculum and Guidelines for Graduate Degree Programs in Information Systems

This article contains the official text of the MSIS 2000 model curriculum as approved by both the Association for Computing Machinery and the Association for Information Systems. It is presented here in its original form