Geometric signature of reversal modes in ferromagnetic nanowires

Magnetic nanowires are a good platform to study fundamental processes in Magnetism and have many attractive applications in recording such as perpendicular storage and in spintronics such as non-volatile magnetic memory devices (MRAM) and magnetic logic devices. In this work, nanowires are used to study magnetization reversal processes through a novel geometric approach. Reversal modes imprint a definite signature on a parametric curve representing the locus of the critical switching field. We show how the different modes affect the geometry of this curve depending on the nature of the anisotropy (uniaxial or cubic anisotropy), demagnetization and exchange effects. The samples we use are electrochemically grown Nickel and Cobalt nanowires.Comment: 11 pages, 21 figures to submit to Europhysics Letter

Parametric Potential Determination by the Canonical Function Method

The canonical function method (CFM) is a powerful means for solving the Radial Schrodinger Equation. The mathematical difficulty of the RSE lies in the fact it is a singular boundary value problem. The CFM turns it into a regular initial value problem and allows the full determination of the spectrum of the Schrodinger operator without calculating the eigenfunctions. Following the parametrisation suggested by Klapisch and Green, Sellin and Zachor we develop a CFM to optimise the potential parameters in order to reproduce the experimental Quantum Defect results for various Rydberg series of He, Ne and Ar as evaluated from Moore's data

Organized complexity of the urban object

Over a half-century, space syntax has proven resilient as a theory and method for describing and analyzing the built environment from dwellings and complex buildings to cities. The paper briefly discusses resilience as a concept in the built environment and the foundations of space syntax itself. We summarize the body of the theoretical thinking in space syntax – laws of the urban object, generic function, principles of centrality and linearity, the design method of spatio-formal processes, and laws of spatial emergence-convergence – before offering a new hypothesis about laws of spatial conservation and spatial optimization at work in the built environment. The latter builds on Conroy-Dalton’s (2001) ideas about angularity and the conservation of linearity in movement. Both could provide an essential bridge with Carvalho and Penn’s (2004) concept of self-similarity in settlements, which relates to Batty and Longley’s (1994) notions of fractal cities. We argue the hypothesis of conservation-optimization defines the conceptual framework for the progressive and regressive practice of urban planning in settlements. We illustrate this theoretical discussion by demonstrating the resilience or replication of previous space syntax findings, and by drawing on new research about the history, spatial structure, and neighborhood logic of Metropolitan Doha.

A DNA damage-induced phosphorylation circuit enhances Mec1ATR Ddc2ATRIP recruitment to Replication Protein A

The cell cycle checkpoint kinase Mec

Nmrk2 gene is upregulated in dilated cardiomyopathy and required for cardiac function and nad levels during aging

Dilated cardiomyopathy (DCM) is a disease of multifactorial etiologies, the risk of which is increased by male sex and age. There are few therapeutic options for patients with DCM who would benefit from identification of common targetable pathways. We used bioinformatics to identify the Nmrk2 gene involved in nicotinamide adenine dinucleotde (NAD) coenzyme biosynthesis as activated in different mouse models and in hearts of human patients with DCM while the Nampt gene controlling a parallel pathway is repressed. A short NMRK2 protein isoform is also known as muscle integrin binding protein (MIBP) binding the α7β1 integrin complex. We investigated the cardiac phenotype of Nmrk2-KO mice to establish its role in cardiac remodeling and function. Young Nmrk2-KO mice developed an eccentric type of cardiac hypertrophy in response to pressure overload rather than the concentric hypertrophy observed in controls. Nmrk2-KO mice developed a progressive DCM-like phenotype with aging, associating eccentric remodeling of the left ventricle and a decline in ejection fraction and showed a reduction in myocardial NAD levels at 24 months. In agreement with involvement of NMRK2 in integrin signaling, we observed a defect in laminin deposition in the basal lamina of cardiomyocytes leading to increased fibrosis at middle age. The α7 integrin was repressed at both transcript and protein level at 24 months. Nmrk2 gene is required to preserve cardiac structure and function, and becomes an important component of the NAD biosynthetic pathways during aging. Molecular characterization of compounds modulating this pathway may have therapeutic potential

Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes

Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability of other efficiently expressed luciferases using different substrates. Here, we characterized a codon-optimized luciferase from Vargula hilgendorfii (Vluc) as a reporter for mammalian gene expression. We showed that Vluc can be multiplexed with Gluc and Fluc for sequential imaging of three distinct cellular phenomena in the same biological system using vargulin, coelenterazine, and D-luciferin substrates, respectively. We applied this triple imaging system to monitor the effect of soluble tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) delivered using an adeno-associated viral vector (AAV) on brain tumors in mice. Vluc imaging showed efficient sTRAIL gene delivery to the brain, while Fluc imaging revealed a robust antiglioma therapy. Further, nuclear factor-κB (NF-κB) activation in response to sTRAIL binding to glioma cells death receptors was monitored by Gluc imaging. This work is the first demonstration of trimodal in vivo bioluminescence imaging and will have a broad applicability in many different fields including immunology, oncology, virology, and neuroscience