Effect of equivalent salt deposit density on flashover voltage of contaminated insulator energized by HVDC

In Malaysia, the demand for electric power is increasing day by day due to more consumption of power in the industrial sector. Recently, the high voltage DC transmission lines are under construction near the coastal environments for transmitting the power to the all states of Malaysia. Therefore, there is a concern about the reliability of these systems especially under adverse environmental conditions due to sea salt spray contamination. This reliability of this contaminated insulator can be improved through its performance studies. For this performance study, an analytical expression between flashover voltage and ESDD of the contaminated insulator has been proposed using Dimensional Analysis technique. The results obtained from the analytical expression are compared with the experimental results and in close agreement are foun

The Challenges and Opportunities in the Implementation of E-learning for Competence Development of State Civil Apparatus

Despite its common utilization in developed countries, e-Learning is relatively new in intensive ASN training in Indonesia. The utilization of e-Learning has increased sharply due to the Covid-19 response protocol that imposes restrictions on physical interaction. This study aims to reveal the challenges and opportunities in implementing e-learning in ASN training in Indonesia during this pandemic. This study applied a descriptive approach. The quantitative data was collected through surveys, while the qualitative data was collected through interviews. The data were obtained from widyaiswara (trainers), organizers, participants, and alums of the 2020 CPNS Basic Training held at the ASN Cadre Development Center of the National Institute of Public Administration (LAN). The findings show that widyaiswara, organizers, and participants, in general, can adapt to e-Learning, even though it is relatively new and sudden and apt to have several challenges. The adaptability results in the excellent progress of the training initially designed with the classical model. It implies the existence of opportunities to overcome several difficulties identified to ensure that e- Learning can become an integral part of the ASN training system in Indonesia

Pressure Corrections for the Potential Flow Analysis of Electrohydrodynamic Kelvin-Helmholtz Instability

The present paper deals with the study of the pressure corrections to the viscous potential flow analysis of Kelvin-Helmholtz instability with tangential electric field at the interface of two viscous fluids. Viscosity enters through normal stress balance in the viscous potential flow theory and tangential stresses for two fluids are not continuous at the interface. Here we have considered viscous pressure in the normal stress balance along with the irrotational pressure and it is assumed that the addition of this viscous pressure will resolve the discontinuity between the tangential stresses and the tangential velocities at the interface of two fluids. The viscous pressure is derived by mechanical energy balance equation and this pressure correction applied to compute the growth rate of electrohydrodynamic Kelvin-Helmholtz instability. A dispersion relation is obtained and stability criterion is given in the terms of critical value of relative velocity. It has been observed that the inclusion of irrotational shearing stresses have stabilizing effect on the stability of the system

The Telegraph Equation and Its Solution by Reduced Differential Transform Method

One-dimensional second-order hyperbolic telegraph equation was formulated using Ohm’s law and solved by a recent and reliable semianalytic method, namely, the reduced differential transform method (RDTM). Using this method, it is possible to find the exact solution or a closed approximate solution of a differential equation. Three numerical examples have been carried out in order to check the effectiveness, the accuracy, and convergence of the method. The RDTM is a powerful mathematical technique for solving wide range of problems arising in science and engineering fields

Complex and unexpected dynamics in simple genetic regulatory networks

Peer reviewedPublisher PD

Stable Maintenance of Multiple Plasmids in E. coli Using a Single Selective Marker

Plasmid-based genetic systems in Escherichia coli are a staple of synthetic biology. However, the use of plasmids imposes limitations on the size of synthetic gene circuits and the ease with which they can be placed into bacterial hosts. For instance, unique selective markers must be used for each plasmid to ensure their maintenance in the host. These selective markers are most often genes encoding resistance to antibiotics such as ampicillin or kanamycin. However, the simultaneous use of multiple antibiotics to retain different plasmids can place undue stress on the host and increase the cost of growth media. To address this problem, we have developed a method for stably transforming three different plasmids in E. coli using a single antibiotic selective marker. To do this, we first examined two different systems with which two plasmids may be maintained. These systems make use of either T7 RNA polymerase-specific regulation of the resistance gene or split antibiotic resistance enzymes encoded on separate plasmids. Finally, we combined the two methods to create a system with which three plasmids can be transformed and stably maintained using a single selective marker. This work shows that large-scale plasmid-based synthetic gene circuits need not be limited by the use of multiple antibiotic resistance genes

Genomic mining of prokaryotic repressors for orthogonal logic gates

Genetic circuits perform computational operations based on interactions between freely diffusing molecules within a cell. When transcription factors are combined to build a circuit, unintended interactions can disrupt its function. Here, we apply 'part mining' to build a library of 73 TetR-family repressors gleaned from prokaryotic genomes. The operators of a subset were determined using an in vitro method, and this information was used to build synthetic promoters. The promoters and repressors were screened for cross-reactions. Of these, 16 were identified that both strongly repress their cognate promoter (5- to 207-fold) and exhibit minimal interactions with other promoters. Each repressor-promoter pair was converted to a NOT gate and characterized. Used as a set of 16 NOT/NOR gates, there are >10[superscript 54] circuits that could be built by changing the pattern of input and output promoters. This represents a large set of compatible gates that can be used to construct user-defined circuits.United States. Air Force Office of Scientific Research (Award FA9550-11-C-0028)American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowship (32 CFR 168a)United States. Defense Advanced Research Projects Agency. Chronical of Lineage Indicative of Origins (N66001-12-C-4016)United States. Office of Naval Research (N00014-13-1-0074)National Institutes of Health (U.S.) (GM095765)National Science Foundation (U.S.). Synthetic Biology Engineering Research Center (SA5284-11210

Biomolecular Filters for Improved Separation of Output Signals in Enzyme Logic Systems Applied to Biomedical Analysis

Biomolecular logic systems processing biochemical input signals and producing "digital" outputs in the form of YES/NO were developed for analysis of physiological conditions characteristic of liver injury, soft tissue injury and abdominal trauma. Injury biomarkers were used as input signals for activating the logic systems. Their normal physiological concentrations were defined as logic-0 level, while their pathologically elevated concentrations were defined as logic-1 values. Since the input concentrations applied as logic 0 and 1 values were not sufficiently different, the output signals being at low and high values (0, 1 outputs) were separated with a short gap making their discrimination difficult. Coupled enzymatic reactions functioning as a biomolecular signal processing system with a built-in filter property were developed. The filter process involves a partial back-conversion of the optical-output-signal-yielding product, but only at its low concentrations, thus allowing the proper discrimination between 0 and 1 output values

SYNZIP Protein Interaction Toolbox: in Vitro and in Vivo Specifications of Heterospecific Coiled-Coil Interaction Domains

The synthetic biology toolkit contains a growing number of parts for regulating transcription and translation, but very few that can be used to control protein association. Here we report characterization of 22 previously published heterospecific synthetic coiled-coil peptides called SYNZIPs. We present biophysical analysis of the oligomerization states, helix orientations, and affinities of 27 SYNZIP pairs. SYNZIP pairs were also tested for interaction in two cell-based assays. In a yeast two-hybrid screen, >85% of 253 comparable interactions were consistent with prior in vitro measurements made using coiled-coil microarrays. In a yeast-signaling assay controlled by coiled-coil mediated scaffolding, 12 SYNZIP pairs were successfully used to down-regulate the expression of a reporter gene following treatment with α-factor. Characterization of these interaction modules dramatically increases the number of available protein interaction parts for synthetic biology and should facilitate a wide range of molecular engineering applications. Summary characteristics of 27 SYNZIP peptide pairs are reported in specification sheets available in the Supporting Information and at the SYNZIP Web site [http://keatingweb.mit.edu/SYNZIP/].National Science Foundation (U.S.) (NSF award MCB 0950233)National Institutes of Health (U.S.) (grant RO1 GM55040)National Institutes of Health (U.S.) (grant PN2 EY016546)National Institutes of Health (U.S.) (grant P50 GMO81879)National Science Foundation (U.S.). Synthetic Biology Engineering Research CenterHoward Hughes Medical Institut