Growth inhibition of neurospora by canavanine, and its reversal

Canavanine, an amino acid from jack beans, was discovered by Kitagawa and coworkers in 1929 (1, 2). The substance is not combined in the proteins of the seed, but occurs in the free state, and makes up 2.5 per cent of the dry weight of jack beans (3). In a series of papers available to the authors for the most part in abstract only, the Japanese workers have reported extensive investigations into the chemistry and physiology of the substance. The structure of canavanine was established by Gulland and Morris (4) and by Kitagawa and Takani (5) as NH2•C(:NII)•NII•O•CH2•CH2•CHNH2•COOH. Natural canavanine is of the L configuration (6)

Genetic basis for an aberrant ascus in N. crassa

Genetic basis for an aberrant ascus in N. crass

Procedure for preparing individual asci of Neurospora for electron microscopy

Procedure for preparing individual asci of Neurospora for electron microscop

Formation of heterokaryons by fusion of isolated hyphal tips on solid medium in petri plates

Formation of heterokaryons by fusion of isolated hyphal tips on solid medium in petri plate

Phenocopies of Neurospora mutants induced by biotin deficiency

Phenocopies of Neurospora mutants induced by biotin deficienc

Ascospore arrangements in the aberrant asci of a Neurospora crassa mutant

Ascospore arrangements in aberrant asci of N. crassa mutan

Spore and ascus mutants in N. tetrasperma

Spore and ascus mutants in N. tetrasperm

Giant spore, a new developmental mutant of N. crassa

Giant spore, a new developmental mutant of N. crass

Microelectrophoresis of extracts from single perithecia

Microelectrophoresis of extracts from single peritheci

The genetic production of small, homokaryotic spores in Neurospora tetrasperma

The genetic production of small, homokaryotic spores in Neurospora tetrasperm