Virtual Environments Research in the Air Force Institute of Technology Virtual Environments, 3-D Medical Imaging, and Computer Graphics Laboratory

The Air Force Institute of Technology Virtual Environments, 3-D Medical Imaging, and Computer Graphics Laboratory is investigating the 3-D computer graphics, user-interface design, networking protocol, and software architecture aspects of distributed virtual environments. In this paper we describe the research projects that are underway in the laboratory. These projects include the development of an aircraft simulator for a distributed virtual environment, projects for observing, analyzing, and understanding virtual environments, a space virtual environment, a project that incorporates “live” aircraft range data into a distributed virtual environment, a virtual environment application framework, and a project for use in a hospital emergency department. We also discuss the research equipment infrastructure in the laboratory, recent publications, and the educational services we provide

Approved for the Major Department

committee and by majority vote has been found to be satisfactory

Immunofluorescence staining of K-fibers in mouse oocytes using cold fixation

The kinetochore is a multiprotein complex that assembles on centromeric DNA and constitutes the main attachment interface between chromosomes and microtubules of the spindle apparatus. Kinetochores also provide the platform for integrating the surveillance mechanism known as the spindle assembly checkpoint (SAC) that regulates the timing of anaphase onset. Saturation of microtubule binding sites on kinetochores displaces SAC proteins leading to loss of SAC-mediated inhibition and the triggering of anaphase. Microtubule binding sites become saturated by bundles of microtubules attached in an end-on manner to kinetochores, termed kinetochore fibers or K-fibers. The appearance of K-fibers therefore signifies the completion of attachment between kinetochores and microtubules and the silencing of the SAC. Here we describe a method involving cold-fixation for immunostaining and imaging K-fibers during meiosis I in mouse oocytes