172 research outputs found
Symmetric Linear Backlund Transformation for Discrete BKP and DKP equation
Proper lattices for the discrete BKP and the discrete DKP equaitons are
determined. Linear B\"acklund transformation equations for the discrete BKP and
the DKP equations are constructed, which possesses the lattice symmetries and
generate auto-B\"acklund transformationsComment: 18 pages,3 figure
Backlund transformations for difference Hirota equation and supersymmetric Bethe ansatz
We consider GL(K|M)-invariant integrable supersymmetric spin chains with
twisted boundary conditions and elucidate the role of Backlund transformations
in solving the difference Hirota equation for eigenvalues of their transfer
matrices. The nested Bethe ansatz technique is shown to be equivalent to a
chain of successive Backlund transformations "undressing" the original problem
to a trivial one.Comment: 22 pages, 2 figures, based on the talk given at the Workshop
"Classical and Quantum Integrable Systems", Dubna, January 200
A Symmetric Generalization of Linear B\"acklund Transformation associated with the Hirota Bilinear Difference Equation
The Hirota bilinear difference equation is generalized to discrete space of
arbitrary dimension. Solutions to the nonlinear difference equations can be
obtained via B\"acklund transformation of the corresponding linear problems.Comment: Latex, 12 pages, 1 figur
Purified F-ATP synthase forms a Ca2+-dependent high-conductance channel matching the mitochondrial permeability transition pore
The molecular identity of the mitochondrial megachannel (MMC)/permeability transition pore (PTP), a key effector of cell death, remains controversial. By combining highly purified, fully active bovine F-ATP synthase with preformed liposomes we show that Ca2+ dissipates the H+ gradient generated by ATP hydrolysis. After incorporation of the same preparation into planar lipid bilayers Ca2+ elicits currents matching those of the MMC/PTP. Currents were fully reversible, were stabilized by benzodiazepine 423, a ligand of the OSCP subunit of F-ATP synthase that activates the MMC/PTP, and were inhibited by Mg2+ and adenine nucleotides, which also inhibit the PTP. Channel activity was insensitive to inhibitors of the adenine nucleotide translocase (ANT) and of the voltage-dependent anion channel (VDAC). Native gel-purified oligomers and dimers, but not monomers, gave rise to channel activity. These findings resolve the long-standing mystery of the MMC/PTP and demonstrate that Ca2+ can transform the energy-conserving F-ATP synthase into an energy-dissipating device
T-systems and Y-systems in integrable systems
The T and Y-systems are ubiquitous structures in classical and quantum
integrable systems. They are difference equations having a variety of aspects
related to commuting transfer matrices in solvable lattice models, q-characters
of Kirillov-Reshetikhin modules of quantum affine algebras, cluster algebras
with coefficients, periodicity conjectures of Zamolodchikov and others,
dilogarithm identities in conformal field theory, difference analogue of
L-operators in KP hierarchy, Stokes phenomena in 1d Schr\"odinger problem,
AdS/CFT correspondence, Toda field equations on discrete space-time, Laplace
sequence in discrete geometry, Fermionic character formulas and combinatorial
completeness of Bethe ansatz, Q-system and ideal gas with exclusion statistics,
analytic and thermodynamic Bethe ans\"atze, quantum transfer matrix method and
so forth. This review article is a collection of short reviews on these topics
which can be read more or less independently.Comment: 156 pages. Minor corrections including the last paragraph of sec.3.5,
eqs.(4.1), (5.28), (9.37) and (13.54). The published version (JPA topical
review) also needs these correction
Legionella Metaeffector Exploits Host Proteasome to Temporally Regulate Cognate Effector
Pathogen-associated secretion systems translocate numerous effector proteins into eukaryotic host cells to coordinate cellular processes important for infection. Spatiotemporal regulation is therefore important for modulating distinct activities of effectors at different stages of infection. Here we provide the first evidence of โmetaeffector,โ a designation for an effector protein that regulates the function of another effector within the host cell. Legionella LubX protein functions as an E3 ubiquitin ligase that hijacks the host proteasome to specifically target the bacterial effector protein SidH for degradation. Delayed delivery of LubX to the host cytoplasm leads to the shutdown of SidH within the host cells at later stages of infection. This demonstrates a sophisticated level of coevolution between eukaryotic cells and L. pneumophila involving an effector that functions as a key regulator to temporally coordinate the function of a cognate effector protein
Protein Crosslinking by Transglutaminase Controls Cuticle Morphogenesis in Drosophila
Transglutaminase (TG) plays important and diverse roles in mammals, such as blood coagulation and formation of the skin barrier, by catalyzing protein crosslinking. In invertebrates, TG is known to be involved in immobilization of invading pathogens at sites of injury. Here we demonstrate that Drosophila TG is an important enzyme for cuticle morphogenesis. Although TG activity was undetectable before the second instar larval stage, it dramatically increased in the third instar larval stage. RNA interference (RNAi) of the TG gene caused a pupal semi-lethal phenotype and abnormal morphology. Furthermore, TG-RNAi flies showed a significantly shorter life span than their counterparts, and approximately 90% of flies died within 30 days after eclosion. Stage-specific TG-RNAi before the third instar larval stage resulted in cuticle abnormality, but the TG-RNAi after the late pupal stage did not, indicating that TG plays a key role at or before the early pupal stage. Immediately following eclosion, acid-extractable protein from wild-type wings was nearly all converted to non-extractable protein due to wing maturation, whereas several proteins remained acid-extractable in the mature wings of TG-RNAi flies. We identified four proteinsโtwo cuticular chitin-binding proteins, larval serum protein 2, and a putative C-type lectinโas TG substrates. RNAi of their corresponding genes caused a lethal phenotype or cuticle abnormality. Our results indicate that TG-dependent protein crosslinking in Drosophila plays a key role in cuticle morphogenesis and sclerotization
Identification of mTEC precursor cells
Medullary thymic epithelial cells (mTECs) expressing autoimmune regulator (Aire) are critical for preventing the onset of autoimmunity. However, the differentiation program of Aire-expressing mTECs (Aire+ mTECs) is unclear. Here, we describe novel embryonic precursors of Aire+ mTECs. We found the candidate precursors of Aire+ mTECs (pMECs) by monitoring the expression of receptor activator of nuclear factor-ฮบB (RANK), which is required for Aire+ mTEC differentiation. pMECs unexpectedly expressed cortical TEC molecules in addition to the mTEC markers UEA-1 ligand and RANK and differentiated into mTECs in reaggregation thymic organ culture. Introduction of pMECs in the embryonic thymus permitted long-term maintenance of Aire+ mTECs and efficiently suppressed the onset of autoimmunity induced by Aire+ mTEC deficiency. Mechanistically, pMECs differentiated into Aire+ mTECs by tumor necrosis factor receptor-associated factor 6-dependent RANK signaling. Moreover, nonclassical nuclear factor-ฮบB activation triggered by RANK and lymphotoxin-ฮฒ receptor signaling promoted pMEC induction from progenitors exhibiting lower RANK expression and higher CD24 expression. Thus, our findings identified two novel stages in the differentiation program of Aire+ mTECs
Multiple Organ System Defects and Transcriptional Dysregulation in the Nipbl+/โ Mouse, a Model of Cornelia de Lange Syndrome
Cornelia de Lange Syndrome (CdLS) is a multi-organ system birth defects disorder linked, in at least half of cases, to heterozygous mutations in the NIPBL gene. In animals and fungi, orthologs of NIPBL regulate cohesin, a complex of proteins that is essential for chromosome cohesion and is also implicated in DNA repair and transcriptional regulation. Mice heterozygous for a gene-trap mutation in Nipbl were produced and exhibited defects characteristic of CdLS, including small size, craniofacial anomalies, microbrachycephaly, heart defects, hearing abnormalities, delayed bone maturation, reduced body fat, behavioral disturbances, and high mortality (75โ80%) during the first weeks of life. These phenotypes arose despite a decrease in Nipbl transcript levels of only โผ30%, implying extreme sensitivity of development to small changes in Nipbl activity. Gene expression profiling demonstrated that Nipbl deficiency leads to modest but significant transcriptional dysregulation of many genes. Expression changes at the protocadherin beta (Pcdhb) locus, as well as at other loci, support the view that NIPBL influences long-range chromosomal regulatory interactions. In addition, evidence is presented that reduced expression of genes involved in adipogenic differentiation may underlie the low amounts of body fat observed both in Nipbl+/โ mice and in individuals with CdLS
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