Genome-wide Integrative Analysis of Zika-Virus-Infected Neuronal Stem Cells Reveals Roles for MicroRNAs in Cell Cycle and Stemness.

Zika virus (ZIKV) infection is implicated in severe fetal developmental disorders, including microcephaly. MicroRNAs (miRNAs) post-transcriptionally regulate numerous processes associated with viral infection and neurodegeneration, but their contribution to ZIKV pathogenesis is unclear. We analyzed the mRNA and miRNA transcriptomes of human neuronal stem cells (hNSCs) during infection with ZIKV MR766 and Paraiba strains. Integration of the miRNA and mRNA expression data into regulatory interaction networks showed that ZIKV infection resulted in miRNA-mediated repression of genes regulating the cell cycle, stem cell maintenance, and neurogenesis. Bioinformatics analysis of Argonaute-bound RNAs in ZIKV-infected hNSCs identified a number of miRNAs with predicted involvement in microcephaly, including miR-124-3p, which dysregulates NSC maintenance through repression of the transferrin receptor (TFRC). Consistent with this, ZIKV infection upregulated miR-124-3p and downregulated TFRC mRNA in ZIKV-infected hNSCs and mouse brain tissue. These data provide insights into the roles of miRNAs in ZIKV pathogenesis, particularly the microcephaly phenotype

On the Variation of Fourier Parameters for Galactic and LMC Cepheids at Optical, Near-Infrared and Mid-Infrared Wavelengths

We present a light curve analysis of fundamental-mode Galactic and Large Magellanic Cloud (LMC) Cepheids based on the Fourier decomposition technique. We have compiled light curve data for Galactic and LMC Cepheids in optical ({\it VI}), near-infrared ({\it JHK}$_s$) and mid-infrared (3.6 $\&$ 4.5-$\mu$m) bands from the literature and determined the variation of their Fourier parameters as a function of period and wavelength. We observed a decrease in Fourier amplitude parameters and an increase in Fourier phase parameters with increasing wavelengths at a given period. We also found a decrease in the skewness and acuteness parameters as a function of wavelength at a fixed period. We applied a binning method to analyze the progression of the mean Fourier parameters with period and wavelength. We found that for periods longer than about 20 days, the values of the Fourier amplitude parameters increase sharply for shorter wavelengths as compared to wavelengths longer than the $J$-band. We observed the variation of the Hertzsprung progression with wavelength. The central period of the Hertzsprung progression was found to increase with wavelength in the case of the Fourier amplitude parameters and decrease with increasing wavelength in the case of phase parameters. We also observed a small variation of the central period of the progression between the Galaxy and LMC, presumably related to metallicity effects. These results will provide useful constraints for stellar pulsation codes that incorporate stellar atmosphere models to produce Cepheid light curves in various bands.Comment: 22 pages, 19 figures, Accepted for publication in Monthly Notices of the Royal Astronomical Society Main Journa

Correlations in the properties of static and rapidly rotating compact stars

Correlations in the properties of the static compact stars (CSs) and the ones rotating with the highest observed frequency of 1122Hz are studied using a large set of equations of state (EOSs). These EOSs span various approaches and their chemical composition vary from the nucleons to hyperons and quarks in $\beta$-equilibrium. It is found that the properties of static CS, like, the maximum gravitational mass $M_{\rm max}^{\rm stat}$ and radius $R_{1.4}^{\rm stat}$ corresponding to t he canonical mass and supramassive or non-supramassive nature of the CS rotating at 1122 Hz are strongly correlated. In particular, only those EOSs yield the CS rotating at 1122Hz to be non-supramassive for which \left (\frac{M_{\rm max}^{\rm stat}}{M_\odot}\right )^{1/2} \left (\frac{10{\rm km}}{R_{1.4}^{\rm stat}})^{3/2} is greater than unity. Suitable parametric form which can be used to split the $M_{\rm max}^{\rm stat}$ $-$ $R_{1.4}^{\rm stat}$ plane into the regions of different supramassive nature of the CS rotating at 1122Hz is presented. Currently measured maximum gravitational mass 1.76$M_\odot$ of PSR J0437-4715 suggests that the CS rotating at 1122Hz can be non-supramassive provided $R_{1.4}^{\rm stat} \leqslant 12.4$ km.Comment: 13 pages, 4 figures, Appearing in Phys. Rev.