Occupational Therapists’ Perspectives on Family-Centered Practices in Early Intervention

Background: Early intervention (EI) requires service provision in natural settings while incorporating interventions based on family-centered practice (FCP). This study sought to understand (a) how occupational therapists define and implement FCP in their daily interventions and (b) therapists’ perspectives on using this model of practice in EI. Method: This study used a qualitative, phenomenological approach. Nine licensed occupational therapists from six states with a minimum of 3 years of working experience in the area of EI participated. Semi-structured interviews were recorded, transcribed, and subsequently coded and analyzed for emergent themes. Results: The therapists had an average of 12.6 years of experience in EI. Four themes were identified: (a) confusion on meaning and implementation of FCP, (b) FCP creates feelings of insecurity, (c) FCP requires therapists to assume roles and engage in activities or practices for which they are not prepared, and (d) systemic issues affect the ability to implement FCP in EI. Conclusion: The participants reported limited evidence-based practice guidelines on FCP models and emphasized the need for training to have a commonality in defining and implementing FCP. Findings indicate a need to address systemic issues affecting how services are approved, delivered, and funded

Connected Rhythm: A Scoping Review of Therapeutic Drumming as an Intervention for Autistic Individuals

Background: Autism Spectrum Disorder (ASD) is a neurologically-based developmental disorder representing a subdomain of neurodivergence. Occupational therapy intervention for ASD typically incorporates sensory modalities, addressing a multitude of challenges, including mental health outcomes. This scoping review analyzes available evidence of the sensory-based intervention of therapeutic drumming (TD) to improve mental health in autistic clients. Method: Following the Johanna Briggs methodological framework, Proquest, Ebsco, Scopus and OTseeker databases were searched with the key and related words of “autism,” “therapeutic drumming,” and “mental health,” yielding 448 articles. After removal of duplicates and application of inclusion criteria, seventeen articles were included in the final analysis. Results: Analysis revealed strong consistency in delivery and implementation of TD intervention. Instrumentation of outcome measures, in contrast, was not well-synchronized; therefore, efficacy of the intervention was difficult to assess. Conclusion: Limitations included a general lack of research, limited research of TD with autistic people in occupational therapy literature, and a dearth of strongly instrumented research. This review revealed valuable contributions of TD interventions as a neurodiversity-affirming practice to support mental health and inform future studies of implementation of TD for people with ASD across the lifespan within the scope of occupational therapy

Effectiveness and cost-effectiveness of body psychotherapy in the treatment of negative symptoms of schizophrenia - a multi-centre randomised controlled trial

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Molecular and cellular characterization of ABCG2 in the prostate

BACKGROUND: Identification and characterization of the prostate stem cell is important for understanding normal prostate development and carcinogenesis. The flow cytometry-based side population (SP) technique has been developed to isolate putative adult stem cells in several human tissue types including the prostate. This phenotype is mainly mediated by the ATP-binding cassette membrane transporter ABCG2. METHODS: Immunolocalization of ABCG2 was performed on normal prostate tissue obtained from radical prostatectomies. Normal human prostate SP cells and ABCG2(+ )cells were isolated and gene expression was determined with DNA array analysis and RT-PCR. Endothelial cells were removed by pre-sorting with CD31. RESULTS: ABCG2 positive cells were localized to the prostate basal epithelium and endothelium. ABCG2(+ )cells in the basal epithelium constituted less than 1% of the total basal cell population. SP cells constituted 0.5–3% of the total epithelial fraction. The SP transcriptome was essentially the same as ABCG2(+ )and both populations expressed genes indicative of a stem cell phenotype, however, the cells also expressed many genes in common with endothelial cells. CONCLUSION: These results provide gene expression profiles for the prostate SP and ABCG2(+ )cells that will be critical for studying normal development and carcinogenesis, in particular as related to the cancer stem cell concept

Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy

BACKGROUND:Mesenchymal progenitor cells (MPCs) have been isolated from a variety of connective tissues, and are commonly called "mesenchymal stem cells" (MSCs). A stem cell is defined as having robust clonal self-renewal and multilineage differentiation potential. Accordingly, the term "MSC" has been criticised, as there is little data demonstrating self-renewal of definitive single-cell-derived (SCD) clonal populations from a mesenchymal cell source. METHODOLOGY/PRINCIPAL FINDINGS:Here we show that a tractable MPC population, human umbilical cord perivascular cells (HUCPVCs), was capable of multilineage differentiation in vitro and, more importantly, contributed to rapid connective tissue healing in vivo by producing bone, cartilage and fibrous stroma. Furthermore, HUCPVCs exhibit a high clonogenic frequency, allowing us to isolate definitive SCD parent and daughter clones from mixed gender suspensions as determined by Y-chromosome fluorescent in situ hybridization. CONCLUSIONS/SIGNIFICANCE:Analysis of the multilineage differentiation capacity of SCD parent clones and daughter clones enabled us to formulate a new hierarchical schema for MSC self-renewal and differentiation in which a self-renewing multipotent MSC gives rise to more restricted self-renewing progenitors that gradually lose differentiation potential until a state of complete restriction to the fibroblast is reached