Performative Polarization: The Interactional and Cultural Drivers of Political Antagonism

Political backlash against liberal democracy and ubiquitous clashes between different versions of identity politics in recent years evoked a heightened awareness of political polarization. Rather than examining the mechanics of this process, social science predominantly conceives political polarization in a rather static manner and measures its prevalence and causes within and between societies. This article views political polarization as taking shape in the experience of political conflict. It proposes a cultural performance framework suitable to examine the social drama of political conflict and its connections to interpersonal political dispute. Performative polarization is premised upon antagonizing one public in order to win over and energize another public. It views political antagonism as constituted by (1) powerful performers and performances that provide the preparatory symbolic work and scripts and (2) divided publics who arbitrate their dramatic acts in ensuing performances and who collectively generate political divisions. The anti-Critical Race Theory campaign in the USA serves as a case study to work through the elements of this theoretical framework

Anotação funcional da família gênica Dof no genoma da videira.

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Meinungsfreiheit an der Universität

In unserer Antwort gehen wir auf einige Kritikpunkte an unserer Studie „Is Free Speech in Danger on University Campus?“ ein, die uns wiederholt und in ähnlicher Form in Diskussionen begegnen. Diese betreffen unsere Fallauswahl, unser Verständnis und unsere Operationalisierung von Toleranz und Meinungsfreiheit sowie nicht zuletzt unsere „Positionalität“. Wie wir darlegen, beruht diese Kritik vornehmlich auf einer verzerrten Rezeption unserer Befunde und auf uns zugeschriebenen Argumentationen, die wir nicht getätigt haben. Wir bekräftigen noch einmal die Relevanz, sich sozialwissenschaftlich mit der Frage nach der Meinungsfreiheit an der Universität auseinanderzusetzen

From Counter-Power to Counter-Pepe: The Vagaries of Participatory Epistemology in a Digital Age

This article reconstructs the evolution of societal and journalistic meta-discourse about the participation of ordinary citizens in the news production process. We do so through a genealogy of what we call “participatory epistemology,” defined here as a form of journalistic knowledge in which professional expertise is modified through 19 public interaction. It is our argument that the notion of “citizen participation in news process” has not simply functioned as a normative concept but has rather carried with it a particular understanding of what journalists could reasonably know, and how their knowledge could be enhanced by engaging with the public in order to produce journalistic work. By examining four key moments in the evolution of participatory epistemology, as well as the discursive webs that have surrounded these moments, we aim to demonstrate some of the factors which led a cherished and utopian concept to become a dark and dystopian one. In this, we supplement the work of Quandt (2018, this issue) and add some historical flesh to the conceptual arguments of his article on “dark participation.

Análise global da expressão de genes Dof em videira.

Neste trabalho é caracterizada a família gênica Dof em videira (Vitis vinifera L.), a qual é composta por 25 genes. A busca por domínios conservados foi realizada utilizando-se o programa MEME suite v.4.9.0. Estudos filogenéticos foram conduzidos comparando 163 sequências Dof de videira, álamo, Arabidopsis thaliana e tomate com proteínas Dof de função conhecida previamente descritos na literatura

Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR.

The aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that infect grapevines in Brazil: Grapevine leafroll-associated virus (GLRaV-1, -2, -3 and -5), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV). The reactions consisted of individual (simplex) and simultaneous (duplex) virus detections. Thirty six grapevine accessions, regenerated after thermotherapy and tissue culture treatments, have been analysed. All the above-mentioned viruses were sensitively detected in simplex reactions in samples infected with different virus isolates. Specifically to GLRaV-1 it was necessary to mix reagents refered by different sources to achieve the amplification. GVA, GRSPaV, GLRaV-2 and GLRaV-3 combined with GVB, GFLV, GFkV, GVD and GLRaV-5 were accurately detected in duplex trials. It was shown, that real-time RT-PCR (TaqMan) is able to efficiently detect different local virus species and isolates.Comunicação científica

Detecção por RT-PCR em Tempo Real e caracterização molecular do Grapevine virus D e Grapevine leafroll-associated virus 5 em videira.

O objetivo deste trabalho foi verificar a ocorrência destes dois vírus em videiras por meio de RT-PCR em Tempo Real, além de caracterizar molecularmente três dos isolados obtidos.Resumo

Reference genes for transcriptional analysis of flowering and fruit ripening stages in apple (Malus 3 domestica Borkh.).

Apple (Malus 9 domestica Borkh.) is the most important deciduous tree fruit crop grown around the world. Comparisons of gene expression profiles from different tissues, conditions or cultivars are valuable scientific tools to better understand the gene expression changes behind important silvicultural and nutritional traits. However, the accuracy of techniques employed to access gene expression is dependent on the evaluation of stable reference genes for data normalization to avoid statistical significance undue or incorrect conclusions. The objective of this work was to select the best genes to be used as references for gene expression studies in apple trees by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Vegetative and reproductive tissues of the apple ??Gala?? cultivar were evaluated during their seasonal cycle of growth and dormancy. The expression of 23 traditional housekeeping genes or genes suggested as constitutive by microarray data was investigated. Tested combinations of primers allowed the specific amplification and the generation of suitable efficiency curves for gene expression studies by RT-qPCR. Gene stability was determined by two different statistical descriptors, geNorm and Norm-Finder. The known variable PAL gene expression was used to validate selected normalizers. Results obtained allowed us to conclude that MDH, SAND, THFS, TMp1 and WD40 are the best reference genes to accurately normalize the relative transcript abundances using RT-qPCR in various tissues of apple.DOI 10.1007/s11032-014-0078-

Reference gene selection for gene expression studies in apple.

Apple (Malus x domestica Borkh.) is the most important deciduous tree fruit crop grown around the world, and also in the Southern of Brazil. Researches on apple genetic breeding include disease resistance mechanisms, grafting, chilling requirement, fruit ripening and production of nutraceutical compounds.Resumo

Transcriptomic characterization of bud dormancy in apple.

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