Revealing the respiratory system of the coffee berry borer (Hypothenemus hampei; Coleoptera: Curculionidae: Scolytinae) using micro-computed tomography

The coffee berry borer (Hypothenemus hampei) is the most economically important insect pest of coffee globally. Micro-computed tomography (micro-CT) was used to reconstruct the respiratory system of this species for the first time; this is the smallest insect (ca. 2 mm long) for which this has been done to date. Anatomical details of the spiracles and tracheal tubes are described, images presented, and new terms introduced. The total volume and the relationship between tracheal lumen diameter, length and volume are also presented. The total length of the tracheal tubes are seventy times the length of the entire animal. Videos and a 3D model for use with mobile devices are included as supplementary information; these could be useful for future research and for teaching insect anatomy to students and the public in general.This paper benefitted from the sub-award agreement S15192.01 between Kansas State University (KSU) and the University of Granada, as part of a USDANIFA Award 2014-70016-23028 to S.J. Brown (KSU), “Developing an Infrastructure and Product Test Pipeline to Deliver Novel Therapies for Citrus Greening Disease” (2015–2020)

Anatomical study of the female reproductive system and bacteriome of Diaphorina citri Kuwayama, (Insecta: Hemiptera, Liviidae) using micro-computed tomography

Huanglongbing (HLB) (citrus greening disease) is one of the most serious bacterial diseases of citrus. It is caused by (1) Candidatus Liberibacter africanus, transmitted by Trioza erytreae and (2) C.L. asiaticus and C.L. americanus, transmitted by Diaphorina citri. As part of a multidisciplinary project on D. citri (www.citrusgreening.org), we made a detailed study, using micro-computed tomography, of the female abdominal terminalia, reproductive system (ovaries, accessory glands, spermatheca, colleterial (= cement) gland, connecting ducts, and ovipositor) and bacteriome, which we present here. New terms and structures are introduced and described, particularly concerning the spermatheca, ovipositor and bacteriome. The quality of images and bacteriome reconstructions are comparable, or clearer, than those previously published using a synchrotron or fuorescence in situ hybridisation (FISH). This study: reviews knowledge of the female reproductive system and bacteriome organ in D. citri; represents the frst detailed morphological study of D. citri to use micro-CT; and extensively revises existing morphological information relevant to psylloids, hemipterans and insects in general. High quality images and supplementary videos represent a signifcant advance in knowledge of psylloid anatomy and are useful tools for future research and as educational aids.Kansas State University (KSU) S15192.01University of Granada, USDA-NIFA S15192.01 2014-70016-2302

Coccidia infections in homing pigeons of various age during the racing season

Coccidiosis caused by Eimeria spp. is a common parasitic disease in homing pigeons. The study objective was to evaluate the incidence of coccidia infections in pigeon lofts during racing season. The intensity of coccidiosis was determined by floatation analyses of faeces samples collected from three pigeon groups performed in three replications (before the racing season, in mid-season and after the end of racing season). The presence of coccidia oocysts was determined in all faeces samples in each replication. At the end of the racing season, the average oocyst counts in faeces samples collected from pigeons that were flown for the first time increased by around 10% in relation to oocysts counts determined before the race. In flown pigeons (aged 2–4 years) a 2.5–9.9% drop was noted in oocysts counts subject to flock, whereas an increase of 15.7–17.3% was reported in parent flocks (unflown pigeons). The results of the experiment indicate that coccidia infections are a common problem in homing pigeons during racing season, which affects racing results and contributes to bird loss

An architecture and methodology for the design and development of Technical Information Systems

In order to meet demands in the context of Technical Information Systems (TIS) pertaining to reliability, extensibility, maintainability, etc., we have developed an architectural framework with accompanying methodological guidelines for designing such systems. With the framework, we aim at complex multiapplication information systems using a repository to share data among applications. The framework proposes to keep a strict separation between Man-Machine-Interface and Model data, and provides design and implementation support to do this effectively. The framework and methodological guidelines have been developed in the context of the ESPRIT project IMPRESS. The project also provided for ldquotesting groundsrdquo in the form of a TIS for the Spanish Electricity company Iberdrola. This work has been conducted within the ESPRIT project IMPRESS (Integrated, Multi-Paradigm, Reliable and Extensible Storage System), ESPRIT No. 635