10 research outputs found
Specific binding of large aggregates of amphiphilic molecules to the respective antibodies
The Binding of nonylphenol to respective antibodies immobilized on solid substrates was studied with the methods of total internal reflection ellipsometry (TIRE) and QCM (quartz crystal microbalance) impedance spectroscopy. The binding reaction was proved to be highly specific having an association constant of K-A = 1.6 x 10(6) mol(-1) L and resulted in an increase in both the adsorbed layer thickness of 23 nm and the added mass of 18.3 mu g/cm(2) at saturation. The obtained responses of both TIRE and QCM methods are substantially higher than anticipated for the immune binding of single molecules of nonylphenol. The mechanism of binding of large aggregates of nonylphenol was suggested instead. Modeling of the micelle of amphiphilic nonylphenol molecules in aqueous solutions yielded a micelle size of about 38 nm. The mechanism of binding of large molecular aggregates to respective antibodies can be extended to other hydrophobic low-molecular-weight toxins such as T-2 mycotoxin. The formation of large molecular aggregates of nonylphenol and T-2 mycotoxin molecules on the surface was proved by the AFM study
Plasmon resonance-enhanced internal reflection ellipsometry for the trace detection of mercuric ion
Application of In Situ IR Ellipsometry in Silicon Electrochemistry to Study Ultrathin Films
Total Internal Reflection Fluorescence Spectroscopy for Investigating the Adsorption of a Porphyrin at the Glass/Water Interface in the Presence of a Cationic Surfactant Below the Critical Micelle Concentration
Rapid and Sensitive Polarization Measurement for Characterizing Protein Adsorption at the Solid–Liquid Interface
Glutathione Immunosensing Platform Based on Total Internal Reflection Ellipsometry Enhanced by Functionalized Gold Nanoparticles
Determination of a setup correction function to obtain adsorption kinetic data at stagnation point flow conditions
A Surface Scientist's view on Spectroscopic Ellipsometry
none1noneMaurizio CanepaCanepa, Maurizi