7 research outputs found
Role du Systeme Nerveux Sur la Proliferation Cellulaire des Blastemes de Regeneration de Membre de Triton (Pleurodeles Waltlii Michah). (regeneration/denervation/proliferation/amphibian limb)
Nerve dependent sulphated glycosaminoglycan synthesis in limb regeneration of the newt Pleurodeles waltl
Nerve-independent DNA synthesis and mitosis in regenerating hindlimbs of larval Xenopus laevis
Transferrin is necessary and sufficient for the neural effect on growth in amphibian limb regeneration blastemas
Nerve-blastema interactions induce fibroblast growth factor-1 release during limb regeneration in Pleurodeles waltl
Regulation of Axolotl (Ambystoma mexicanum) Limb Blastema Cell Proliferation by Nerves and BMP2 in Organotypic Slice Culture
We have modified and optimized the technique of organotypic slice culture in order to study the mechanisms regulating growth and pattern formation in regenerating axolotl limb blastemas. Blastema cells maintain many of the behaviors that are characteristic of blastemas in vivo when cultured as slices in vitro, including rates of proliferation that are comparable to what has been reported in vivo. Because the blastema slices can be cultured in basal medium without fetal bovine serum, it was possible to test the response of blastema cells to signaling molecules present in serum, as well as those produced by nerves. We also were able to investigate the response of blastema cells to experimentally regulated changes in BMP signaling. Blastema cells responded to all of these signals by increasing the rate of proliferation and the level of expression of the blastema marker gene, Prrx-1. The organotypic slice culture model provides the opportunity to identify and characterize the spatial and temporal co-regulation of pathways in order to induce and enhance a regenerative response