ANTI-MELANOMA ACTIVITY OF PERPHENAZINE AND PROCHLORPERAZINE IN HUMAN COLO829 AND C32 CELL LINES

Cutaneous melanoma is fairly common (only about 1% of skin cancers), but is the deadliest malignant tumor. Moreover, amelanotic type of melanoma is very difficult for clinical diagnosis. The standard therapy can cause a lot of side effects e.g. nausea, vomiting and headaches, which means that novel and effective strategies are required. Interestingly, phenothiazine derivatives possess sedative, antiemetic, and anticancer activity. In the present study, we explore the anticancer effect of perphenazine and prochlorperazine in human melanotic (COLO829) and amelanotic (C32) melanoma cells suggesting that prochlorperazine inhibits cell viability in a concentration-dependent manner, impairs migration, and decreases tyrosinase and MITF amount. Moreover, the analyzed drugs decrease/increase MITF amount depending on type of melanoma. We demonstrated that the decrease of MITF and tyrosinase protein induces migration inhibition of C32 cells, and suggests the ability of those drugs to restore cancer cell sensitivity to treatment. The ability of prochlorperazine to containing the spread of the amelanotic melanoma in vivo may be helpful in the development of a new and effective antimelanoma therapy

ANTI-MELANOMA ACTIVITY OF PERPHENAZINE AND PROCHLORPERAZINE IN HUMAN COLO829 AND C32 CELL LINES

Cutaneous melanoma is fairly common (only about 1% of skin cancers), but is the deadliest malignant tumor. Moreover, amelanotic type of melanoma is very difficult for clinical diagnosis. The standard therapy can cause a lot of side effects e.g. nausea, vomiting and headaches, which means that novel and effective strategies are required. Interestingly, phenothiazine derivatives possess sedative, antiemetic, and anticancer activity. In the present study, we explore the anticancer effect of perphenazine and prochlorperazine in human melanotic (COLO829) and amelanotic (C32) melanoma cells suggesting that prochlorperazine inhibits cell viability in a concentration-dependent manner, impairs migration, and decreases tyrosinase and MITF amount. Moreover, the analyzed drugs decrease/increase MITF amount depending on type of melanoma. We demonstrated that the decrease of MITF and tyrosinase protein induces migration inhibition of C32 cells, and suggests the ability of those drugs to restore cancer cell sensitivity to treatment. The ability of prochlorperazine to containing the spread of the amelanotic melanoma in vivo may be helpful in the development of a new and effective antimelanoma therapy.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Fire – a Factor Forming the Numbers of Microorganisms and Mesofauna in Forest Soils

Jednym z czynników stresogennych poważnie zakłócającym równowagę biologiczną gleby jest pożar. Charakter zaburzeń powodowanych przez pożar zależy od siły, czasu trwania, sezonu i częstotliwości jego pojawiania się. Pożary wpływają na fizyczne, chemiczne i biologiczne własności gleby. Przyczyniają się do zmniejszenia liczebności mezo – i makrofauny oraz wpływają na mikroorganizmy glebowe. Celem podjętych badań była ocena wpływu intensywności pożaru na liczebność wybranych grup edafonu glebowego w tym bakterii heterotroficznych, grzybów mikroskopowych oraz mezofauny glebowej roztoczy (Acari) i skoczogonek (Collembola) w ściółce i w glebie na pożarzysku w Puszczy Kampinoskiej. Podczas pożaru powierzchniowego (jaki miał miejsce w Puszczy Kampinoskiej) organizmy zasiedlające wierzchnie warstwy gleby, są bezpośrednio narażone na działanie pożaru. Założono, że liczebność organizmów związanych z warstwą organiczną gleb powinna być znacznie niższa na obszarach wypalonych w porównaniu z niewypalonymi. Do badań wytypowano stanowiska po mocnym i słabym pożarze oraz nie objęte ogniem oddalone o 10 m od linii ognia – stanowiące kontrolę, każda o łącznej powierzchni 10 m2. Na podstawie uzyskanych wyników stwierdzono, że właściwości chemiczne i biologiczne gleby ulegają stopniowej regeneracji w glebie i ściółce w 15 miesięcy po pożarze. Ponadto intensywność pożaru ma istotne znaczenie w odtwarzaniu liczebności mikroorganizmów i mezofauny. Słaby pożar działa bardziej selekcjonująco na mikroorganizmy i mezofaunę, niż pożar mocny. Uzyskane wyniki badań uprawniają do stwierdzenia, że pożar może być jednym z czynników stymulujących liczebność grzybów zarówno w poziomie organicznym, jak i mineralnym gleb leśnych.One of the stressors that seriously disturbs the biological balance of soil is fire. The nature of disorder caused by the fire depends on the strength, duration, season and the frequency of occurrence. Fires affect physical, chemical and biological properties of soil. They contribute to reducing the population of meso- and macrofauna and affect micro-organisms living in the soil. The purpose of the study was to evaluate the effect of fire intensity on the number of selected soil edafon groups including heterotrophic bacteria, microscopic fungi, Acari and Collembola, both in the leaf litter and soil in Kampinos Forest. During the surface fire (which took place in Kampinos National Park) organisms inhabiting the surface of the soil were directly exposed to fire. It was assumed that the number of organisms in the organic layer should be considerably lower in areas burned compared to non-burned. Research was conducted on sites that had encountered high severity fires, low severity fires and on those not affected - 10 m from the fire line - each with a total area of 10 m2. Based on the obtained results, it was found that the chemical and biological properties of soil gradually recovered in the soil and litter within 15 months from the fire. In addition, the intensity of the fire is important in reproducing the population of microorganisms and mesofauna. A low severity fire works more selectively on microorganisms and mesofauna than a severe one. The results of the research indicate that the fire can be one of the factors stimulating fungal growth both in the organic and mineral layers of the forest soils