29 research outputs found
CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model
Differences in joint morphology between the knee and ankle affect the repair of osteochondral defects in a rabbit model
Dysfunction in Sertoli cells participates in glucocorticoid‐induced impairment of spermatogenesis
Facile method to covalently-jointed ZnO-NPs with Chitosan by using GPTMS: a new strategy to decrease the cytotoxicity of ZnO-NPs
Effect of Bone Morphogenetic Protein-2 on Normal and Osteoarthritic Human Articular Chondrocytes
Mechanical responses and integrin associated protein expression by human ankle chondrocytes
Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers
Expression of thrombospondin-1 (TSP-1) and signal-regulatory protein-alpha (SIRPα) by human articular chondrocytes and molecular associations of CD47/IAP
Protein extracts from primary cultures of human articular chondrocytes were separated by SDS-PAGE and immunoblotted with antibodies against TSP-1 and SIRPα. For TSP-1, a 6% gel under reducing conditions was used, whereas for SIRPα, an 8% gel under non-reducing conditions was used. Protein extracts from primary cultures of human articular chondrocytes were immunoprecipitated with antibodies against CD47/IAP and immunoblotted for TSP-1, SIRPα, or α5 integrin. TSP-1, SIRPα, and α5 integrin are identified in non-immunoprecipitated extracts, but only TSP-1 and α5 integrin co-immunoprecipitate with CD47/IAP. IP, immunoprecipitated protein extracts; MW, molecular weight; N, normal human articular chondrocytes; Non IP, non-immunoprecipitated protein extracts; OA, osteoarthritic human articular chondrocytes.<p><b>Copyright information:</b></p><p>Taken from "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an model"</p><p>http://arthritis-research.com/content/10/1/R4</p><p>Arthritis Research & Therapy 2008;10(1):R4-R4.</p><p>Published online 10 Jan 2008</p><p>PMCID:PMC2374443.</p><p></p