A novel anti-inflammatory activity of lysozyme: modulation of serum complement activation.

Lysozyme is an ubiquitous enzyme found in most biological secretions and leukocytes. This study was aimed at investigating its interaction with other inflammatory mediators on mucosa surfaces, particularly the complement system. Lysozyme has been shown in our present study, to inhibit the haemolytic activity of serum complement in a dose-dependent fashion, when tested within the levels present in normal and inflamed breast-milk samples, and other mucosal secretions. This represents a new anti-inflammatory action of lysozyme in relation to the serum complement, and the exact mode of the interaction need further studies

The effects of construction on environmental resources in peninsular Malaysia: the perspective of land use planning

Environment forms the basis upon which all human activities such as physical, social and economic take place. Malaysia is a nation that is blessed with the beauty of nature and at the same time advanced in terms of infrastructural development. Environmental resources are vital components of the environment that include land, vegetal resource, water bodies, atmosphere, flora and fauna. Nowadays, due to high rate of urbanization, rapid population and economic growth led to greater need of space for operation, which subsequently forced the construction industries to meet this demand. This gives rise to various forms of environmental degradations which lead to disruption of environmental equilibrium. The study assesses the effects of construction industries on the environment in the perspectives of land use planning. However, if proper measures are not taken, the consequences will never be of benefit to the country. Land use planning is an approach used to identify the relationship between construction activities and environmental resources. Generally, the study area covers Peninsula Malaysia because of the similarities of construction conditions and the environmental effects. The methodology employed in this study is based on the available data derived from the secondary sources of information. It also aims at suggesting ways of achieving sustainable construction activities so that our natural earth will not be harmed, destroyed or degraded

ASSESSMENT OF GRAIN STORAGE TECHNOLOGIES FOR EFFECTIVE MARKETING IN SUSTAINING FOOD SECURITY PROGRAMME BY TRADERS IN SOUTHWEST NIGERIA

Nigerian Food Security Programme is centred on three-tier grain storage with active participation of traders in storage of 85% of grain requirements through the On-Farm Storage Programme. The study assessed grain storage technologies to determine suitable ones for storage and marketing by traders in Southwest Nigeria. A pre-data survey of recommended grain storage technologies was followed by multi- stage sampling of Oyo, Ondo and Ogun States for 120 rural and urban traders. Data were analysed with descriptive and inferential statistics at p = 0.05. Traders preferred recommended storage technologies except silo. Only sacks were preferred out of the indigenous storage technologies. Technology attributes and communication factors are essential for use of recommended storage technologies. There is no significant relationship between age (r = 0.86), income (r = 0.78) and use of recommended storage technologies while quantity of grains stored (r = 0.94), years of experience in grain storage (r = 0.93) and educational status (X2 = 9.51) were significantly related.  Rural and urban traders were not significantly different in their levels of use of recommended grain storage technologies (tc = 0.20). Traders’ storage extension through the use of various channels of communication, trainings and adult education programme were recommended. Key words: Recommended technologies, indigenous technologies, determinants, use

A Decision Support System for Information Technology Policy Formulation

The implementation of an effective ICT policy requires the development of material and intellectual resources to support good decision making by humans.   In this paper, we examined and analysed Information Technology (IT) policy development process with a view to developing automated system supporting such process.  The data used for this work were obtained through purposeful interview of five professionals and experts who are familiar with IT policy formulation in Nigerian environment.  Some of the experts had earlier participated in policy design and formulation process at national level.     The Hierarchical Input Process Output (HIPO) model was used to analyse various input (contributions of professionals and experts) and output (agreed resolution of the professionals and experts) of the system.  The information obtained from the experts was represented using rule base techniques.  The overall system was designed using the Unified Modelling Language (UML) and implemented using the Visual Prolog version 7.0.  The metrics used for evaluating the system includes: processing time, decision process efficiency and cost effectiveness.   We compared the result of our system with that of the traditional manual system in use.  Our result showed that the DSS for policy formulation process enhances the decision output significantly when compared to the manual process where no DSS is used.  Moreover, the quality of policy produced by our DSS system is more consistent when compared with the manual process.

An Assessment of the Impact of Disruptive Technologies on the Efficacy of Accounting Practices in Selected South Western States, Nigeria

Advancement in technology has brought intense but healthy competition among professional bodies in Nigeria, especially in the field of accounting. This has systematically transformed the accounting process from a traditional analog to a digital system. It is on this note that this study examined how disruptive technology affects the efficacy of accounting practice in Nigeria. This study employed a survey research method with the use of a structured questionnaire distributed among professional bodies in Ekiti, Osun, and Ondo States, South Western Nigeria. Regression analysis of Ordinary Least Squares coupled with correlation analysis were employed. The results revealed that artificial intelligence, blockchain, big data, and the internet of things had a significant positive effect on the controlled variable in Nigeria. The results also revealed that cloud computing had insignificant negative effect on the dependent variable. With the F Statistics (7.113) = 109.747, P = 0.000 < 0.05), the results showed a significantly strong relationship between the controlling and controlled variables. It is, thus, recommended the pivotal need for accounting practitioners to enhance their knowledge of disruptive technologies through training and retraining, and continuous attendance of related workshops organized by the respective professional bodies in Nigeria

Effects of Farmers’ Demographic Factors on the Adoption of Grain Storage Technologies Developed by Nigerian Stored Products Research Institute (NSPRI): A case study of selected villages in Ilorin West LGA of Kwara State.

This study was carried out to identify the factors that determine farmers’ adoption of NSPRI grain storage technologies in selected villages of Ilorin West Local Government Area of Kwara State. Data used were collected from a total of 120 respondents who were randomly selected from 6 villages of the Local Government Area. 10% of all the farmers in each of the villages were interviewed. The variables examined in the study are sex, age, marital status, primary occupation, secondary occupation, level of education, number of dependents, years of farming, farm size, years of grain production, means of farm land acquisition and types of grain produced. Descriptive statistics and logistic regression model were used to analyze data. The inferential statistical tool used indicated significant relationship between sex and NSPRI grain storage technologies’ adoption.  Level of education and occupation were also significantly related to adoption of NSPRI grains storage technologies. This study recommends a wholesome dissemination model that will benefit both educated and uneducated farmer and also the involvement of farmers’ group or representatives in participatory approach of technology development, introduction and use. Keywords: Education, farming, occupation, participatory development, logit regression model

Neural and behavioural changes in male periadolescent mice after prolonged nicotine-MDMA treatment

The interaction between MDMA and Nicotine affects multiple brain centres and neurotransmitter systems (serotonin, dopamine and glutamate) involved in motor coordination and cognition. In this study, we have elucidated the effect of prolonged (10 days) MDMA, Nicotine and a combined Nicotine-MDMA treatment on motor-cognitive neural functions. In addition, we have shown the correlation between the observed behavioural change and neural structural changes induced by these treatments in BALB/c mice.We observed that MDMA (2 mg/Kg body weight; subcutaneous) induced a decline in motor function, while Nicotine (2 mg/Kg body weight; subcutaneous) improved motor function in male periadolescent mice. In combined treatment, Nicotine reduced the motor function decline observed in MDMA treatment, thus no significant change in motor function for the combined treatment versus the control. Nicotine or MDMA treatment reduced memory function and altered hippocampal structure. Similarly, a combined Nicotine-MDMA treatment reduced memory function when compared with the control. Ultimately, the metabolic and structural changes in these neural systems were seen to vary for the various forms of treatment. It is noteworthy to mention that a combined treatment increased the rate of lipid peroxidation in brain tissue

IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function

In rats, learning and memory performance decline during normal aging, which makes this rodent species a suitable model to evaluate therapeutic strategies. In aging rats, insulin-like growth factor-I (IGF-I), is known to significantly improve spatial memory accuracy as compared to control counterparts. A constellation of gene expression changes underlie the hippocampal phenotype of aging but no studies on the effects of IGF-I on the hippocampal transcriptome of old rodents have been documented. Here, we assessed the effects of IGF-I gene therapy on spatial memory performance in old female rats and compared them with changes in the hippocampal transcriptome. In the Barnes maze test, experimental rats showed a significantly higher exploratory frequency of the goal hole than controls. Hippocampal RNA-sequencing showed that 219 genes are differentially expressed in 28 months old rats intracerebroventricularly injected with an adenovector expressing rat IGF-I as compared with placebo adenovector-injected counterparts. From the differentially expressed genes, 81 were down and 138 upregulated. From those genes, a list of functionally relevant genes, concerning hippocampal IGF-I expression, synaptic plasticity as well as neuronal function was identified. Our results provide an initial glimpse at the molecular mechanisms underlying the neuroprotective actions of IGF-I in the aging brain.Instituto de Investigaciones Bioquímicas de La PlataFacultad de Ciencias Médica

Potentials of microorganisms associated with plantain peels in the Lagos metropolis for biodegradation and bioconversion.

The role of microbes in the degradation of plantain derived-wastes and their potential to produce cellulolytic enzymes was assessed. Soil samples of decomposing waste piles were collected from two major plantain markets in the Lagos metropolis and analyzed for physicochemical properties, toxic heavy metal content and microbial populations. Findings revealed that the values of moisture content of the two soils varied between 7.27±0.04 and 8.06±0.19 %. M-12 site had the highest organic matter content of 6.89±0.14 %. A similar pattern was observed for nitrate, phosphate and chloride levels while some heavy metals were also detected in varying and high amounts. The highest viable bacterial counts was 58.0±2.9 x 104 cfu/g at MU and there were no fungi at the site whereas M-12 had a fungal count of 40.0±3.3 x 103 cfu/g. Out of the total of 34 isolates encountered, 8 isolates having maximum cellulase activities were selected for further studies by the primary screening technique. These test organisms were then evaluated by secondary screening for enzyme production. The test organisms were phenotypically and biochemically characterized and identified as Klebsiella pneumoniae spp pneumoniae (2 strains), Klebsiella pneumoniae spp ozaenae, Enterobacter aerogenes, Providencia alcalifaciens, Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger respectively. Both the bacteria and moulds were found to be capable of utilizing lignin and cellulosic substrates for growth and for production of cellulolytic enzymes. It is suggested that such microorganisms could be useful in bioconversion of cellulosic substrates like plantain-derived wastes for biotechnological application

Vitamin D3 Receptor Activation Rescued Corticostriatal Neural Activity and Improved Motor-Cognitive Function in −D2R Parkinsonian Mice Model

Background: fourth generation antipsychotics have been implicated in the blockade of calcium signalling through inhibition of dopamine receptive sites on dopaminergic D2 Receptor (D2R). As a result of the abnormal calcium signalling associated with D2R inhibition, changes occur in the motor and memory neural axis leading to the observed behavioural deficits after prolonged haloperidol. Thus, Vitamin D3 receptor (VD3R), a calcium controlling receptor in the striatum can be targeted to relief the neurological symptoms associated with haloperidol (−D2R) induced PD. Aim: This study sets to investigate the role of VD3R activation in vitro and in vivo after haloperidolinduced Dopaminergic (D2R) blockade. In addition, we examined the associated neural activity and behavioural changes in parkinsonian and VDRA intervention mice. Methods: Dopaminergic D2R inhibition was investigated in vitro using Melanocytes isolated from the scale of a Tilapia. In four separate set ups, the cells were cultured in calcium free Ringer’s solution as follows; 300 μM haloperidol, 100 μM VD3, 100 mM calcium chloride and a combination of 300 μM haloperidol and 100 μM VD3. Subsequently, dopaminergic vesicle accumulation and calcium signalling were observed in bright field microscopy using blue and green fluorescence probes. In the second phase, PD was induced in adult BALB/c mice (−D2; n = 8) after 14 days of intraperitoneal haloperidol treatment (10 mg/Kg). A set of n = 4 mice were untreated (−D2) while the other group (n = 4) received 100 mg/Kg of VD3 for 7 days (−D2/+VDR). The control groups (n = 4 each) were treated with normal saline (NS) and VD3 (+VDR) for 14 days. At the end of the treatment phase, the animals were assessed in Rotarod, parallel bar-, cylinder-, Y-Maze-, one trial place recognition- and novel object recognition-(NOR) tests. Neural activity was measured using chronic electrode implants placed in the M1 (motor cortex), CPu (striatum), CA1 (hippocampus) and PFC (prefrontal cortex). Neural activity was compared with the outcomes of behavioural tests for memory and motor functions and data was expressed as mean ± SEM (analysed using ANOVA with Tukey post-hoc test, significant level was set at 0.05). Results/Discussion: in vitro outcomes show that VDR increase calcium signalling and reverses the effect of haloperidol; specifically by reducing dopaminergic vesicle accumulation in the cell body. Similarly, in vivo neural recordings suggest an increase in calcium hyperpolarization currents in the CPu and PFC of intervention mice (−D2/+VDR) when compared with the parkinsonian mice (−D2). These animals (−D2/+VDR) also recorded an improvement in spatial working memory and motor function versus the Parkinsonian mice (−D2). These outcomes suggest the role of CPu-PFC corticostriatal outputs in the motor-cognitive decline seen in parkinsonian mice. Similarly, VDRA reduced the neural deficits through restoration of calcium currents (burst activities) in the intervention mice (−D2/+VDR). Conclusion: VDRA treatment reduced the motor-cognitive defects observed in haloperidol induced PD. Our findings suggest the role of VDRA in restoration of calcium currents associated with PFC and CPu corticostriatal outputs seen as burst frequencies in in vivo neural recording