Magnetic resonance imaging of human-derived amniotic membrane stem cells using PEGylated superparamagnetic iron oxide nanoparticles

Objective: The label and detection of cells injected into target tissues is an area of focus for researchers. Iron oxide nanoparticles can be used to label cells as they have special characteristics. The purpose of this study is to examine the effects of iron oxide nanoparticles on human-derived amniotic membrane stem cell (hAMCs) survival and to investigate the magnetic properties of these nanoparticles with increased contrast in magnetic resonance imaging (MRI). Materials and Methods: In this experimental study, we initially isolated mesenchymal stem cells from amniotic membranes and analyzed them by?ow cytometry. In addition, we synthesized superparamagnetic iron oxide nanoparticles (SPIONs) and characterized them by various methods. The SPIONs were incubated with hAMCs at concentrations of 25-800 μg/mL. The cytotoxicity of nanoparticles on hAMCs was measured by the MTT assay. Next, we evaluated the effectiveness of the magnetic nanoparticles as MRI contrast agents. Solutions of SPION were prepared in water at different iron concentrations for relaxivity measurements by a 1.5 Tesla clinical MRI instrument. Results: The isolated cells showed an adherent spindle shaped morphology. Polyethylene glycol (PEG)-coated SPIONs exhibited a spherical morphology. The average particle size was 20 nm and magnetic saturation was 60 emu/g. Data analysis showed no signifcant reduction in the percentage of viable cells (97.86 ± 0.41) after 72 hours at the 125 μg/ml concentration compared with the control. The relaxometry results of this SPION showed a transverse relaxivity of 6.966 (μg/ml.s)-1 Conclusion: SPIONs coated with PEG used in this study at suitable concentrations had excellent labeling efficiency and biocompatibility for hAMCs

Amniotic membrane mesenchymal stem cells labeled by iron oxide nanoparticles exert cardioprotective effects against isoproterenol (ISO)-induced myocardial damage by targeting inflammatory MAPK/NF-κB pathway

The aim of the present study is to investigate the protective effects of human amniotic membrane-derived mesenchymal stem cells (hAMSCs) labeled by superparamagnetic iron oxide nanoparticles (SPIONs) against isoproterenol (ISO)-induced myocardial injury in the presence and absence of a magnetic field. ISO was injected subcutaneously for 4 consecutive days to induce myocardial injury in male Wistar rats. The hAMSCs were incubated with 100 μg/ml SPIONs and injected to rats in magnet-dependent and magnet-independent groups via the tail vein. The size and shape of nanoparticles were determined by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Prussian blue staining was used to determine cell uptake of nanoparticles. Myocardial fibrosis, heart function, characterization of hAMSCs, and histopathological changes were determined using Masson�s trichrome, echocardiography, flow cytometry, and H&E staining, respectively. Enzyme-linked immunosorbent assay (ELISA) was used to the expression pro-inflammatory cytokines. Immunohistochemistry assay was used to determine the expression of nuclear factor-κB (NF-κB) and the Ras/mitogen-activated protein kinase (MAPK). SPION-labeled MSCs in the presence of magnetic field significantly improved cardiac function and reduced fibrosis and tissue damage by suppressing inflammation in a NF-κB/MAPK-dependent mechanism (p < 0. 05). Collectively, our findings demonstrate that SPION-labeled MSCs in the presence of magnetic field can be a good treatment option to reduce inflammation following myocardial injury. Figure not available: see fulltext. © 2020, Controlled Release Society

The effects of superparamagnetic iron oxide nanoparticles-labeled mesenchymal stem cells in the presence of a magnetic field on attenuation of injury after heart failure

Migration of stem cells after transplantation reduces their therapeutic effects. In this study, we hypothesized that superparamagnetic iron oxide nanoparticles (SPION)-labeled mesenchymal stem cells (MSCs) in the presence of magnetic field may have a capability to increase regenerative ability after heart failure (HF). A rat model of ISO (isoproterenol)-HF was established to investigate the effects of SPION-labeled MSCs on tissue regeneration in the presence and absence of magnetic field. Hydrodynamic size, shape, and formation of chemical bonds between SPION and polyethylene glycol (PEG) were measured using dynamic light scattering (DLS), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). The MRI was used to monitor SPION-labeled MSCs in vivo. Cell and tissue uptake of nanoparticles were determined by Prussian blue staining, atomic absorption spectroscopy (AAS), and inductively coupled plasma spectroscopy (ICP). Purity of the MSCs, heart function, myocardial fibrosis, and histologic damage were evaluated using flow-cytometry, echocardiography, Masson�s trichrome, and H&E staining respectively. Various spectroscopic and microscopic analyses revealed that hydrodynamic size of SPION was 40 ± 2 and their shape was spherical. FTIR confirmed the presence of PEG on the surface of nanoparticles. The presence of magnetic field significantly increased cell homing. Highly purified MSCs population was detected by flow-cytometry. Using SPION-labeled MSCs in the presence of magnetic field markedly improved heart function and myocardial hypertrophy and reduced fibrosis (p < 0.05). Collectively, our results demonstrated that SPION-labeled MSCs in the presence of magnetic field might contribute to regeneration after HF. © 2018, Controlled Release Society

Lavender Oil Attenuates Myocardial Ischemia/Reperfusion Injury Through Inhibition of Autophagy and Stimulation of Angiogenesis

Myocardial infarction (MI) is a serious threat to human health that creates millions of death annually. In this study, we went on to examine molecular mechanism of lavender oil that which by give rises to cardioprotection against MI. An MI model was established in male Wistar rats by ligation of the left anterior descending coronary artery (LAD) and lavender oil at 100 mg/kg concentration 1 h after reperfusion was intraperitoneally administrated. ELISA assay was used to evaluate the activities of myocardial injury markers. Western blot and immunohistochemical assays were used to evaluate the expression of beclin-1, LC3II/LC3I ratio, CD34 and vascular endothelial growth factor (VEGF). RT-PCR was applied to investigate the mRNA levels of apoptotic factors. Compared to sham, increased levels of myocardial injury markers, pro-apoptotic and autophagic factors were found in MI rats that were reversed by post-treatment with lavender oil. Likewise, reduced levels of anti-apoptotic factors and VEGF were observed in MI/R group compared to sham that reversed by lavender oil. Collectively, our findings showed the validity of lavender oil as an excellent candidate to create cardioprotection effects against MI/R injury. © 2021, Shiraz University

Investigation of Exon 1 in FXN Gene in Patients with Clinical Symptomatic of Friedreich Ataxia

Background and Objectives: Friedreich’s ataxia (FRDA) is an autosomal recessive disorder that is typically associated with dysarthria, muscle weakness, spasticity in the lower limbs, scoliosis, bladder dysfunction, absent lower limb reflexes, and loss of position and vibration sense. Approximately two-thirds of these patients suffer from cardiomyopathy and more than 30% have diabetes mellitus. Individuals with FRDA have identifiable mutations in the FXN gene . The most common type of mutation which is observed on both alleles in more than 98% of patients is an expansion of a GAA triplet-repeat in intron of FXN gene. Approximately 2% of individuals with FRDA are compound heterozygotes, who have a GAA expansion in the disease-causing range in one FXN allele and an inactivating FXN mutation in another allele . Aim of the present study was to investigate exon 1 in FRDA gene in patients with clinical symptoms of Friedreich’s Ataxia that have not GAA triplet-repeat expansion in intron 1 of FXN gene.   Methods: In this study , exon 1 in 5 patients suspected of FRDA analyzed using PCR and sequencing.   Results : An A to G transition at nucleotide number 815284, in exon 1 was observed in all patients.   Conclusion: The results of this study showed that disease-causing homozygous mutations could be because of consanguinity marriage in Iran. Therefore, sequencing of all exons of the gene is necessary