50 лет геофизических исследований ледников в Институте географии

In 1967‑2015, Institute of Geography of the USSR/Russian Academy of Sciences together with other organizations carried out field expeditions in different areas of mountain and polar glaciations in many regions: the Polar Urals, Caucasus, Pamir, Zailiysky and Jungar Alatau, Tien‑Shan, Pamir‑Alai, the Kamchatka Peninsula, the Pyrenees, the Arctic – Spitsbergen, Novaya Zemlya, Franz Josef and Severnaya Zemlya, and Antarctica – on the ice flow B, and in the sub‑Antarctic – Islands King George, Galindez, and Livingston. The gravimetric and ground and aerial radar observations were made in these expeditions. About 300 glaciers of different morphological types and sizes with cold, subpolar and temperate thermal regime were studied. Basic results of these studies are the following: (1) the new data on the ice thicknesses, ice volumes, subglacial relief, internal structure, and thermal state of the glaciers were obtained; (2) the two‑layered (polythermal) glaciers consisting of the upper layer of cold ice and the lower layer of temperate water‑filled ice had been revealed in Svalbard for the first time; spatial distribution of cold, polythermal and temperate glaciers had been determined; (3) the evidences were obtained that measured changes in thickness of the upper cold ice layer in polythermal glaciers can be used to estimate the long‑period variations of regional climates and serve as regional paleothermometers; (4) methods for estimating the water content in temperate and polythermal glaciers from the RES data were developed; and its space‑time variations in temperate ices of the Svaldbald glaciers were estimated since even small water content inside of them can noticeably change their dynamic behavior; (5) methods for estimating the ice volume within glaciers in large regions of mountain and polar glaciations had been created; the ice storages were estimated in Svalbard, Franz Josef Land, Dzhungrsky Alatau, the Great Caucasus, and Mt. Elbrus; (6) detailed data on the ice thicknesses and the subglacial relief had been obtained for 40 glaciers in framework of different national and international programs and projects; the data can be used to solve a wide range of practical and theoretical problems, including numerical modeling. These studies demonstrated the following: (1) the use of monopulse radars VIRL‑6 and VIRL‑7 of decameter range (the central frequency is 20 MHz) with digital recording of the radar and GPS data is quite efficient for ground‑based and airborne (from helicopters) radio‑echo sounding of mountain and polar glaciers with their ice thicknesses up to 500–600 m; (2) it was found that thicknesses of glaciers in the Caucasus and Tien Shan can reach 330–430 m, while in regions of mountain, ice‑sheet and transitional glaciation on the Spitsbergen Archipelago – 300, 560 and 600 m, respectively, on the ice caps of the Franz‑Josef Land and Severnaya Zemlya – 450 and 813 m, and on King George and Livingston Islands (Sub‑Antarctica) – 330 and 500 m; (3) large parts of ice caps and outlet glaciers in Svalbard, Franz Josef Land, Severnaya Zemlya which beds were located below the sea level were found. Precisely these parts can be undergone quick shortening due to climate warming, and, thus, cause formation of icebergs making threats for ships and gas‑oil marine platforms in the Barents and Kara seas; (4) data of the measurements made possible to calculate volumes of a number of investigated glaciers and ice caps and to estimate the ice storages in large areas of mountain and polar glaciations (the Jungar Alatau, Great Caucasus, Spitsbergen, Franz Josef Land); (5) decreasing of glacier volumes on the Franz Josef Land and some Spitsbergen glaciers for the last decades had been estimated. Analysis of the data obtained had shown that considerable part of polythermal glaciers in Spitsbergen belong to type of surging glaciers; they have the winter englacial runoff and form the near‑glacier icings. It allows considering such glaciers as dynamically unstable, predisposed to surges as well as possible sources of winter water supply and additional sources of paleoinformation about long‑period variations of regional climate.Рассказано об истории и основных результатах геофизических исследований ледников, выполненных в Институте географии АН СССР/РАН в период с 1966 по 2016 г. группой специалистов по изучению толщины и строения ледников с применением геофизических методов. Такие данные необходимы для оценки запасов пресной воды в ледниках и их вклада в изменение уровня Мирового океана, а также для прогнозирования и реконструкции динамики ледников

Low temperature setting iron phosphate ceramics as a stabilization and solidification agent for incinerator ash contaminated with transuranic and RCRA metals

Incineration of combustible Mixed Transuranic Waste yields an ash residue that contains oxides of Resource Conservation and Recovery Act (RCRA) and transuranic metals. In order to dispose of this ash safely, it has to be solidified and stabilized to satisfy appropriate requirements for repository disposal. This paper describes a new method for solidification of incinerator ash, using room temperature setting iron phosphate ceramics, and includes fabrication procedures for these waste forms as well as results of the MCC-1 static leach test, XRD analysis, scanning electron microscopy studies and density measurements of the solidified waste form produced

Скорость распространения радиоволн в сухом и влажном снежном покрове

In recent years, ground-penetrating radars are widely used for measuring thickness and liquid water content in snow cover on land and glaciers. The measurement accuracy depends on radio wave velocity (RWV) adopted for calculations. The RWV depends mainly on density, water content and structure of the snow cover and ice layers in it. The density and wetness of snow, and its structure can be estimated from data on RWV, using the available experimental and theoretical relations. Satisfactory results can be obtained using the Looyenga’s (1965) equations to estimate the density and wetness of snow cover, and equations of van Beek’s (1967) showing the distinction between RWV speeds velocities in snow cover and ice layers with different prevailing orientation and sizes of air or water inclusions.RWV in dry snow with density 300 kg/m3 may vary by 32 m/µs, depending on whether the vertical or horizontal orientation of the air inclusions prevails therein. In ice with density 700 kg/m3 effect of air inclusions orientation on differences in RWV is reduced to 5 m/µs. If the inclusions are not filled with air but with water, the difference in RWV in snow is 21 m/µs, and in ice is 24 m/µs. The RWV is affected not only by orientation of the inclusions, but their elongation. Twofold elongation of ellipsoidal air and water inclusions increases the difference in RWV in snow (with a density 300 kg/m3 ) to 23 m/µs and 22 m/µs.These estimates show a noticeable influence of snow structure on RWV in snow cover. The reliability of the above RWV estimates depends significantly on a thermal state of the snow cover, and decreases during snowmelt and increases in the cold period. It strongly depends on accuracy of measurements of the RWV in snow cover and its separate layers. With sufficiently high accuracy of the measurements this makes possible to detect and identify loose layers of deep hoar and compact layers of infiltration and superimposed ice, which is important for studying the liquid water storage of snow cover and a glacier mass balance. Therefore, considerable attention should be given to accuracy of the RWV measurements in dry and wet snow cover and its individual layers. With sufficiently high accuracy of measurements of the RWV, this should allow revealing such layers and estimating their thickness and average density.Представлены результаты расчётов скорости распространения радиоволн в снежном покрове в зависимости от плотности, влажности, структуры снега и прослоек в нём льда по разным эмпирическим и теоретическим зависимостям. Различие в скорости распространения радиоволн в сухом снеге плотностью 300  кг/м3 с преобладающей вертикальной или горизонтальной ориентацией включений воздуха достигает 32  м/мкс и уменьшается до 5  м/мкс во льду плотностью 700  кг/м3. Выполненные оценки показывают заметное влияние структуры на скорость распространения радиоволн в сухом и влажном снежном покрове, что позволяет обнаруживать и идентифицировать рыхлые слои глубинной изморози и плотные слои инфильтрационного и наложенного льда

Recommendations for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samples.

Genotyping of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in monitoring viral evolution and transmission during the pandemic. The quality of the sequence data obtained from these genotyping efforts depends on several factors, including the quantity/integrity of the input material, the technology, and laboratory-specific implementation. The current lack of guidelines for SARS-CoV-2 genotyping leads to inclusion of error-containing genome sequences in genomic epidemiology studies. We aimed to establish clear and broadly applicable recommendations for reliable virus genotyping. We established and used a sequencing data analysis workflow that reliably identifies and removes technical artefacts; such artefacts can result in miscalls when using alternative pipelines to process clinical samples and synthetic viral genomes with an amplicon-based genotyping approach. We evaluated the impact of experimental factors, including viral load and sequencing depth, on correct sequence determination. We found that at least 1000 viral genomes are necessary to confidently detect variants in the SARS-CoV-2 genome at frequencies of ≥10%. The broad applicability of our recommendations was validated in over 200 clinical samples from six independent laboratories. The genotypes we determined for clinical isolates with sufficient quality cluster by sampling location and period. Our analysis also supports the rise in frequencies of 20A.EU1 and 20A.EU2, two recently reported European strains whose dissemination was facilitated by travel during the summer of 2020. We present much-needed recommendations for the reliable determination of SARS-CoV-2 genome sequences and demonstrate their broad applicability in a large cohort of clinical samples

Equal Channel Angular Extrusion Progress Report for March 1998 - May 1999

Pure copper and Alloy 5083 aluminum were processed by equal channel angular extrusion (ECAE); their microstructural evolution and corresponding mechanical properties were investigated. Work also began on the possible use of ECAE to synthesize advanced materials or to consolidate metal powders or powder mixtures. The die tooling used for ECAE is described and selected microstructural and mechanical property results for ECAE-processed copper and cold-rolled (conventionally-processed) copper in the as-processed and annealed condition are compared. Results thus far show that the “pure” metal is prone to low temperature recrystallization after large strain hardening—more beneficial effects are expected in the dispersion-strengthened and precipitation-hardening alloys. The large range of tensile properties and grain sizes from the copper allowed a flow stress analysis to be performed. From this analysis, a new model for flow stress behavior is proposed. An evaluation of ECAE processing of material for spot welding electrodes began. Results to date include electrodes of ECAE-processed commercially pure copper (Alloy 101). Future work involving Glidcop® (Al2O3 oxide dispersionstrengthened copper) and CuCrZr (Cr-Zr precipitation dispersion) materials will be required to fully investigate the benefits of ECAE for electrode life extension. Initial work on Aluminum Alloy 5083 showed that ECAE led to grain refinement as well as broke up and more uniformly dispersed the hardening precipitates. This is desirable for enhancing superplastic behavior. Study of ECAE for consolidating metal powder began. Early results with a Cu-Ag powder indicate that near 100% density was achieved with room temperature consolidation

Условия на ложе и поверхности ледникового купола Вавилова (Северная Земля) во время его подвижки по данным аэрорадиозондирования

The glacier surge at Vavilov Ice Cap, Severnaya Zemlya, Russia (79°18′ N, 94°40′ E) began as early as the mid-1960s with a slow advance of its margin in the western part. Since 2012, the advance switched to the phase of catastrophic movement, which reached its climax in 2016, when the glacier velocity reached 9.2 km a‒1. An ice fan with an area of about 140 km2 advanced into the Kara Sea water area 11 km from the shore, and a strongly crevassed ice stream was formed in the ice cap itself, which continues to move now with speeds of about 2 km a‒1. The dynamic instability of Vavilov Ice Cap can be triggered by changes in basal conditions, which are still poorly known. In this study, we used airborne radio-echo sounding data acquired in September 2014 over the ice cap to characterize its surface and bedrock conditions. Based on the delay time and reflection amplitudes, the power reflection coefficient (PRC) from glacier surface and bedrock was estimated. For its calibration, we used the amplitude of reflections from the sea surface registered from different altitudes. The bedrock PRC values were converted to dielectric permittivity and compared with the glacier surface velocities in 2014 obtained from Landsat-7 images. We found a high positive correlation between the bedrock PRCs and velocities in the area with glacier speed higher than 1000 m a-1. In this area, the PRC is 20 dB higher than in the neighboring slower moving areas. Such a difference may be because the ice stream advanced on marine loose sediments with higher dielectric permittivity and conductivity and a higher reflection coefficient. The range of estimated bedrock PRCs corresponds to bed materials with relative dielectric permittivity from 5 to 10 and electrical conductivity from 10–5 to 10–2 Sm m‒1.По данным измерений времени запаздывания и амплитуд радиоотражений от ложа ледникового купола Вавилова, полученным в период быстрой подвижки его западной части, определены значения коэффициента отражения от ложа по мощности, которые коррелируют с высокими скоростями (более 1000 м/год) движения ледника в области, наступившей на участок мелководья

A flow cytometry-based method to simplify the analysis and quantification of protein association to chromatin in mammalian cells.

Protein accumulation on chromatin has traditionally been studied using immunofluorescence microscopy or biochemical cellular fractionation followed by western immunoblot analysis. As a way to improve the reproducibility of this kind of analysis, to make it easier to quantify and to allow a streamlined application in high-throughput screens, we recently combined a classical immunofluorescence microscopy detection technique with flow cytometry. In addition to the features described above, and by combining it with detection of both DNA content and DNA replication, this method allows unequivocal and direct assignment of cell cycle distribution of protein association to chromatin without the need for cell culture synchronization. Furthermore, it is relatively quick (takes no more than a working day from sample collection to quantification), requires less starting material compared with standard biochemical fractionation methods and overcomes the need for flat, adherent cell types that are required for immunofluorescence microscopy.Research in our laboratory is funded by Cancer Research UK (CRUK; programme grant C6/A11224), the European Research Council and the European Community Seventh Framework Programme (grant agreement no. HEALTH¬‐F2¬‐2010¬‐259893 (DDResponse)). Core funding is provided by Cancer Research UK (C6946/A14492) and the Wellcome Trust (WT092096). J.V.F. is funded by Cancer Research UK programme grant C6/A11224 and the Ataxia Telangiectasia Society. S.P.J. receives his salary from the University of Cambridge, supplemented by CRUK.This is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/nprot.2015.06

Modeling double strand break susceptibility to interrogate structural variation in cancer

Abstract Background Structural variants (SVs) are known to play important roles in a variety of cancers, but their origins and functional consequences are still poorly understood. Many SVs are thought to emerge from errors in the repair processes following DNA double strand breaks (DSBs). Results We used experimentally quantified DSB frequencies in cell lines with matched chromatin and sequence features to derive the first quantitative genome-wide models of DSB susceptibility. These models are accurate and provide novel insights into the mutational mechanisms generating DSBs. Models trained in one cell type can be successfully applied to others, but a substantial proportion of DSBs appear to reflect cell type-specific processes. Using model predictions as a proxy for susceptibility to DSBs in tumors, many SV-enriched regions appear to be poorly explained by selectively neutral mutational bias alone. A substantial number of these regions show unexpectedly high SV breakpoint frequencies given their predicted susceptibility to mutation and are therefore credible targets of positive selection in tumors. These putatively positively selected SV hotspots are enriched for genes previously shown to be oncogenic. In contrast, several hundred regions across the genome show unexpectedly low levels of SVs, given their relatively high susceptibility to mutation. These novel coldspot regions appear to be subject to purifying selection in tumors and are enriched for active promoters and enhancers. Conclusions We conclude that models of DSB susceptibility offer a rigorous approach to the inference of SVs putatively subject to selection in tumors

State of the art of immunoassay methods for B-type natriuretic peptides: An update

The aim of this review article is to give an update on the state of the art of the immunoassay methods for the measurement of B-type natriuretic peptide (BNP) and its related peptides. Using chromatographic procedures, several studies reported an increasing number of circulating peptides related to BNP in human plasma of patients with heart failure. These peptides may have reduced or even no biological activity. Furthermore, other studies have suggested that, using immunoassays that are considered specific for BNP, the precursor of the peptide hormone, proBNP, constitutes a major portion of the peptide measured in plasma of patients with heart failure. Because BNP immunoassay methods show large (up to 50%) systematic differences in values, the use of identical decision values for all immunoassay methods, as suggested by the most recent international guidelines, seems unreasonable. Since proBNP significantly cross-reacts with all commercial immunoassay methods considered specific for BNP, manufacturers should test and clearly declare the degree of cross-reactivity of glycosylated and non-glycosylated proBNP in their BNP immunoassay methods. Clinicians should take into account that there are large systematic differences between methods when they compare results from different laboratories that use different BNP immunoassays. On the other hand, clinical laboratories should take part in external quality assessment (EQA) programs to evaluate the bias of their method in comparison to other BNP methods. Finally, the authors believe that the development of more specific methods for the active peptide, BNP1–32, should reduce the systematic differences between methods and result in better harmonization of results