Aniline incorporated silica nanobubbles

We report the synthesis of stearate functionalized nanobubbles of SiO2 with a few aniline molecules inside, represented as C6H5NH2@SiO2@stearate, exhibiting fluorescence with red-shifted emission. Stearic acid functionalization allows the materials to be handled just as free molecules, for dissolution, precipitation, storage etc. The methodology adopted involves adsorption of aniline on the surface of gold nanoparticles with subsequent growth of a silica shell through monolayers, followed by the selective removal of the metal core either using sodium cyanide or by a new reaction involving halocarbons. The material is stable and can be stored for extended periods without loss of fluorescence. Spectroscopic and voltammetric properties of the system were studied in order to understand the interaction of aniline with the shell as well as the monolayer, whilst transmission electron microscopy has been used to study the silica shell

Efficacy of spirotetramat in the control of the currant-sowthistle (Hyperomyzus lactucae L.) on blackcurrant plantations in Poland

ABSTRACT Spirotetramat applied as Movento 100 SC at dose rates: 0.7 and 1.0 L·ha-1 in a single treatment just after blossom resulted in a very high reduction of currant-sowthistle aphid - Hyperomyzus lactucae L. (Aphidoidea, Aphididae) on blackcurrants ‘Ojebyn’ and ‘Titania’. The results obtained with this pesticide applied at both tested dose rates: 0.7 and 1.0 L·ha-1 were comparable. The efficacy of spirotetramat at 7th day after treatment was similar or slightly poorer compared to standard insecticides, but at 21st day after treatment a higher reduction of aphids than with reference insecticides such as pirimicarb (Pirimor 500 WG at the rate 0.75 kg·ha-1), thiacloprid (Calypso 480 SC 0.15 L·ha-1) and thiamethoxam (Actara 25 WG at the rate 0.1 kg·ha-1) was recorded. With regard to environment safety and biodiversity it must be underlined that on plants treated with Movento 100 SC, high populations of Coccinellidae (both larvae and beetles), Syrphidae (larvae) and Phytoseiidae were noted. </jats:p

A large fraction of trisomy 12, 17p ⁻ , and 11q ⁻ CLL cases carry unidentified microdeletions of <i>miR-15a/16-1</i>

Significance 13q14.3 deletion is the most common genetic lesion identified in CLLs. This study shows that microdeletions affecting the miR-15a/16-1 cluster are more frequent than expected in all CLL cohorts and are prevalent in patients carrying a trisomy 12. Copy-number variation analysis and an experimental FISH analysis revealed that ∼34% of samples carry previously unidentified microdeletions of miR-15a/16-1 . These data may have clinical relevance for the successful stratification of patients for treatment. </jats:p

A large fraction of trisomy 12, 17p-, and 11q- CLL cases carry unidentified microdeletions of miR-15a/16-1.

Chronic lymphocytic leukemia (CLL) is the most common adult leukemia and is characterized by chromosomal aberrations including 13q, 11q, and 17p deletions and a trisomy of chromosome 12 (T12). 13q deletions are often associated with 11q and 17p deletions in aggressive cases. Conversely, T12 CLLs show a variable prognosis, and association with 13q deletions is uncommon. The miR-15a/16-1 cluster is the functional target of 13q deletions, leading to BCL2 overexpression. Chromosomal aberrations in CLL are associated with prognosis, and their identification is carried out by fluorescence in situ hybridization (FISH). Since standard FISH only detects large deletions, we investigated the presence of undetected microdeletions targeting miR-15a/16-1 in CLL cases. We found that ∼34% of CLL samples show an unreported loss of the miR-15a/16-1 locus regardless of their cytogenetic profile. Interestingly, 15 out of 39 (∼39%) of all CLLs with T12, carry microdeletions of miR-15a/16-1, indicating that, in patients with T12, miR-15a/16-1 are mostly inactivated by microdeletions. In addition, ∼40% of CLL cases bearing T12, 17p-, and 11q- showed unidentified microdeletions of miR-15a/16-1, suggesting that miR-15a/16-1 loss cooperates with such chromosomal alterations in CLL. These data may have clinical relevance for the successful stratification of patients for treatment