Photometry and low resolution spectroscopy of hot post-AGB candidates

We have obtained Johnson U, B, V and Cousins R, I photometry and low resolution spectra of a small sample of hot post-AGB candidates. Using the present data in combination with JHK data from 2MASS, infrared data from the MSX catalog and the IRAS fluxes, we have studied the spectral energy distribution (SED) of these stars. Using the DUSTY code we have estimated the dust temperatures, the distances to the stars, the mass-loss rates, angular radii of the inner boundary of the dust envelopes and dynamical ages from the tip of the AGB. These candidates have also been imaged through a narrow band H-alpha filter, to search for nebulosity around the central stars. Our H-alpha images revealed the bipolar morphology of the low excitation PN IRAS 17395-0841 with an angular extent of 2.8arcsec. The bipolar lobes of IRAS 17423-1755 in H-alpha were found to have an angular extent of 3.5arcsec (south-east lobe) and 2.2arcsec (north-west lobe). The dust envelope characteristics, low resolution spectrum and IRAS colors suggest that IRAS 18313-1738 is similar to the proto-planetary nebula (PPN) HD 51585. The SED of IRAS 17423-1755, IRAS 18313-1738 and IRAS 19127+1717 show a warm dust component (in addition to the cold dust) which may be due to recent and ongoing mass-loss.Comment: 20 pages, 6 figures, h-alpha figure compressed with XV, paper accepted for publication in Astronomy & Astrophysic

Staggered Spin Order of Localized pi-electrons in the Insulating State of the Organic Conductor kappa-BETS)2Mn[N(CN)2]3

Magnetic properties of the conduction pi-electron system of kappa-BETS)2Mn[N(CN)2]3 have been probed using 13C NMR. At ambient pressure, the metal-insulator transition observed in the resistivity measurements below T~23K is shown to be accompanied by ordering of the pi-spins in a long-range staggered structure. As the metal-insulator transition is suppressed by applying a small pressure of ~0.5 kbar, the pi-spin system maintains the properties of the metallic state down to 5K.Comment: 13 pages, 4 figure

The Human Retinoblastoma Gene Is Imprinted

Genomic imprinting is an epigenetic process leading to parent-of-origin–specific DNA methylation and gene expression. To date, ∼60 imprinted human genes are known. Based on genome-wide methylation analysis of a patient with multiple imprinting defects, we have identified a differentially methylated CpG island in intron 2 of the retinoblastoma (RB1) gene on chromosome 13. The CpG island is part of a 5′-truncated, processed pseudogene derived from the KIAA0649 gene on chromosome 9 and corresponds to two small CpG islands in the open reading frame of the ancestral gene. It is methylated on the maternal chromosome 13 and acts as a weak promoter for an alternative RB1 transcript on the paternal chromosome 13. In four other KIAA0649 pseudogene copies, which are located on chromosome 22, the two CpG islands have deteriorated and the CpG dinucleotides are fully methylated. By analysing allelic RB1 transcript levels in blood cells, as well as in hypermethylated and 5-aza-2′-deoxycytidine–treated lymphoblastoid cells, we have found that differential methylation of the CpG island skews RB1 gene expression in favor of the maternal allele. Thus, RB1 is imprinted in the same direction as CDKN1C, which operates upstream of RB1. The imprinting of two components of the same pathway indicates that there has been strong evolutionary selection for maternal inhibition of cell proliferation

Normalisation to Blood Activity Is Required for the Accurate Quantification of Na/I Symporter Ectopic Expression by SPECT/CT in Individual Subjects

The utilisation of the Na/I symporter (NIS) and associated radiotracers as a reporter system for imaging gene expression is now reaching the clinical setting in cancer gene therapy applications. However, a formal assessment of the methodology in terms of normalisation of the data still remains to be performed, particularly in the context of the assessment of activities in individual subjects in longitudinal studies. In this context, we administered to mice a recombinant, replication-incompetent adenovirus encoding rat NIS, or a human colorectal carcinoma cell line (HT29) encoding mouse NIS. We used 99mTc pertechnetate as a radiotracer for SPECT/CT imaging to determine the pattern of ectopic NIS expression in longitudinal kinetic studies. Some animals of the cohort were culled and NIS expression was measured by quantitative RT-PCR and immunohistochemistry. The radioactive content of some liver biopsies was also measured ex vivo. Our results show that in longitudinal studies involving datasets taken from individual mice, the presentation of non-normalised data (activity expressed as %ID/g or %ID/cc) leads to ‘noisy’, and sometimes incoherent, results. This variability is due to the fact that the blood pertechnetate concentration can vary up to three-fold from day to day. Normalisation of these data with blood activities corrects for these inconsistencies. We advocate that, blood pertechnetate activity should be determined and used to normalise the activity measured in the organ/region of interest that expresses NIS ectopically. Considering that NIS imaging has already reached the clinical setting in the context of cancer gene therapy, this normalisation may be essential in order to obtain accurate and predictive information in future longitudinal clinical studies in biotherapy

On the interpretation of the CH cometary spectrum

A procedure to compute cometary spectra taking into account radiative processes and collisional effects is described. The populations of the rotational levels are determined by solving the statistical equilibrium equations at a certain number of locations within the coma (which can be modelled with n(R), T(R), v(R) laws) and the emerging intensity is then evaluated by integrating the emissions along the line of sight. Such a program was applied to the CH radical. The importance of collisions in exciting the lower rotational levels of CH is confirmed