Study of serum lipid profile and magnesium in preeclampsia

Background: A comparative study of serum lipid profile and magnesium levels in normal pregnancy versus preeclampsia (PE).Methods: A prospective study done for 2 years (October 2014 to October 2016) in the Department of Obstetrics and Gynecology, Narayana medical college and hospital, a tertiary care centre, Nellore, Andhra Pradesh, India. A sample size of 200 pregnant women, recruited and divided into group A and B. group A being women with PE and group B is normal pregnant women.A10ml of venous blood was collected in the fasting state and serum collected from clotted blood to measure lipid profile, magnesium, Apo lipoprotein A-I and Apo lipoprotein B 100.Serum Lipid profile  measured by enzymatic method using commercially available kit Human (GmbH Germany) using humastar 600 chemistry analyzer (Human GmbH Germany). Serum magnesium measured by dye binding method using commercially available kit Human (GmbH Germany) using Humastar 600 chemistry analyzer (Human GmbH Germany). Serum ApoA-I and ApoB were measured by immune-turbidometry using commercial kits from Spinreact Spain. Urine albumin done by dipstick method.Results: Serum total cholesterol (TC), Triglyceride (TG), low density lipoprotein (LDL), very low-density lipoprotein (VLDL), Apo lipoprotein B 100 (Apo B100) were high and serum Magnesium, high density lipoprotein (HDL) and Apo lipoprotein A1(Apo A1) were low in the study group (group A) compared to controls (group B).Conclusions: Abnormal lipid profile (low HDL and increased TG concentration) and serum hypomagnesaemia may be contributing etiologies of preeclampsia, having good predictive value as a screening procedure for PE in high risk pregnant population

Impact of Carbon and Nitrogen Sources on L-Asparaginase Production by Isolated Bacillus circulans (MTCC 8574): Application of Saturated Plackett-Burman Design

The present work aimed to study the impact of different carbon and nitrogen sources on the effective production of L-asparaginase by isolated Bacillus circulans using Plackett-Burman design (PBD). Among carbon sources; lactose, galactose, starch, sucrose, maltose, arabinose and among nitrogen sources; proline, tryptone, sodium glutamate, corn steep liquor, beef extract, ammonium chloride, yeast extract at selected concentration produced a maximum of 105 and 164 U mL–1 L-asparaginase enzyme, respectively. Initial analysis of L-asparaginase data with different carbon and nitrogen sources as per PBD did not reveal standardized effects as well as ‘p’ and ‘t’ values. Regression coefficient and t-values were calculated by subjecting the experimental data to statistical analysis after pooling the least two nutrient components into error. Among selected 26 components, xylose, galactose, yeast extract and proline denoted least significance, while glucose, mannose, ammonium chloride and ammonium sulphate showed maximum significance on enzyme production. Saturated PBD can be effectively utilized for analysis of the data by pooling the least significant factors based on the effects on metabolite/product/enzyme production. Ammonium chloride and glucose were observed to be the most significant carbon and nitrogen sources, respectively in L-asparaginase production in this bacterial strain. The presented approach is highly useful in bioprocess optimization procedure where all selected parameters show little degree of freedom and subsequent significant factors

Seroma: an interesting case report

A seroma is defined as a sterile accumulation of serum in a circumscribed location in the tissue. Seromas can occur after surgeries, especially those that are extensive or involve significant tissue disruption viz. hernia repairs, plastic surgeries such as breast augmentation or reconstruction, abdominoplasties (tummy tucks)and removal of large intra-abdominal space occupying lesions like huge fibroids/ovarian masses. The literature citing the exact incidence of seromas following removal of large intra-abdominal masses is scant. Seromas at times become severely symptomatic. The best way to prevent a seroma is not to give it any place to form. It's easier said than done. Here is an interesting case report of postoperative seroma following removal of a large broad ligament fibroid

Sensory and nutritional quality of Moringa oleifera leaf powder incorporated multi-millet ready to eat (RTE) snack

204-209The present study aimed at developing value-added multi-millet ready to eat (RTE) snack by incorporating Moringa oleifera (Drumstick) leaf powder (MOLP) at different concentrations viz., 0%, 5%, 10% 15% and evaluation of corresponding sensory and nutritional properties. The sensory evaluation revealed that newly formulated 5% MOLP snacks had higher overall sensory acceptability after control snacks. Besides, nutritional values also demonstrated that MOLP (15%) incorporated multi millet snacks contained abundant minerals including iron 99.09±0.6 mg/kg, zinc 20.27±0.3 mg/kg and calcium 4374±22.7 mg/kg. The protein (9.681±0.01 g/100 g) and antioxidants (230.6±0.46 µg/100 g) contents were also (pMoringa powder in RTE snacks production could be used to develop functional food with enhanced minerals, protein and antioxidants

Discovery of selective and potent anti-tubercular activity in arylpentane-1,4-diones

411-415Anti-tubercular activity (MIC 50μg/mL against Mtb H37Rv) have been reported in hispolons, which possess α, β-unsaturated-1,3-diketone functional group. In the pursuit to optimize structure and bioactivity, various substituted arylpentane-1,4-diones S1-8 have been synthesised. Upon screening, the test compounds show negligible antimicrobial activity against the tested organisms at 100 μg/mL but show antitubercular activity against Mycobacterium tuberculosis H37Rv strain with an MIC 0.8 to 25μg/mL. Compounds having a halogen at the ortho position on the aromatic ring show highest potency (MIC 0.8μg/mL), implying a relation between the position of the halogen atom and anti-tubercular activity

Synchronization of Fractional Order Uncertain BAM Competitive Neural Networks

This article examines the drive-response synchronization of a class of fractional order uncertain BAM (Bidirectional Associative Memory) competitive neural networks. By using the differential inclusions theory, and constructing a proper Lyapunov-Krasovskii functional, novel sufficient conditions are obtained to achieve global asymptotic stability of fractional order uncertain BAM competitive neural networks. This novel approach is based on the linear matrix inequality (LMI) technique and the derived conditions are easy to verify via the LMI toolbox. Moreover, numerical examples are presented to show the feasibility and effectiveness of the theoretical results