Validation and quantitation of an in vitro M-cell model

This study has evaluated an in vitro model of the follicle-associated epithelia that overlie Peyer's patches of the small intestine. The model shares many phenotypic characteristics of M- cells in vivo. Co-cultures of the human adenocarcinoma cell line Caco-2 and freshly isolated Peyer's patch cells were established. Fluorescence microscopy and quantitative image analysis were used to validate the model against known markers of M-cell phenotype. Apical expression of alkaline phosphatase was down-regulated in co-cultures and villin was re- distributed from the apical membrane to the cytoplasm. a5ß1 integrin was found on the apical surfaces of the monolayers and B and T lymphocytes integrated into the monolayers. Particle transport was temperature-dependent in co-cultures, indicating that a transcytotic route was responsible. This model provides opportunities to study factors that influence M-cell development, assess putative Peyer's patch targeting in oral vaccine technologies, and study intestinal uptake in vitro

Time-layered cultural map of Australia

This paper reports on an Australian project that is developing an online system to deliver researcher-driven national-scale infrastructure for the humanities, focused on mapping, time series, and data integration. Australian scholars and scholars of Australia worldwide are well served with digital resources and tools to deepen the understanding of Australia and its historical and cultural heritage. There are, however, significant barriers to use. The Time Layered Cultural Map of Australia (TLCMap) will provide an umbrella infrastructure related to time and space, helping to activate and draw together existing high-quality resources. TLCMap expands the use of Australian cultural and historical data for research through sharply defined and powerful discovery mechanisms. See https://tlcmap.newcastle.edu.au/

By staff and for staff: building, sustaining and empowering a community of practice in teaching and learning for all university staff

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