Provocation: Technology, resistance and surveillance in public space

The introduction of technologies that monitor and track individuals to attribute suspicion and guilt has become commonplace in practices of order maintenance in public space. A case study of the introduction of a marker spray in Dutch urban public transport is used to conceptualise the role of technology in everyday resistances against surveillance. The introduction of this technology made available alternative subject positions. The notion of provocation is proposed for the opening up of social spaces by a technology. Through provocation, issues that do not find their expression in commonly accepted protocols and means of evidence are given a voice as a result of defiant, emotional and provisional technology usage. Attending to visible and defiant usages also opens up an agenda for examining the varying intensities at which technology operates

Doing a transversal method: developing an ethics of care in a collaborative research project

Beck and Sznaider call for ‘methodological cosmopolitanism’ to transcend methodological nationalism and account for an increasingly cosmopolitanized reality. We take up their challenge by drawing on our experiences doing a collaborative ethnography of methodological changes in the production of population statistics within and between European national and international statistical institutes. Drawing on debates in Science and Technology Studies, we depart from some conceptual presuppositions of methodological cosmopolitanism to define a ‘transversal method’. Referring to this method as performative and ontopolitical, we reflect on how it requires collaboration and in our ethnography gave rise to three practical challenges: 1) going beyond the individual project; 2) using each other’s field notes; 3) working against the national order of things. To meet these challenges we reflect on how this method required that we practice three modes of care: thinking with others, tinkering with field notes, and dissenting within

Phenotypic Studies of Natural Killer Cell Subsets in Human Transporter Associated with Antigen Processing Deficiency

Peripheral blood natural killer (NK) cells from patients with transporter associated with antigen processing (TAP) deficiency are hyporesponsive. The mechanism of this defect is unknown, but the phenotype of TAP-deficient NK cells is almost normal. However, we noticed a high percentage of CD56bright cells among total NK cells from two patients. We further investigated TAP-deficient NK cells in these patients and compared them to NK cells from two other TAP-deficient patients with no clinical symptoms and to individuals with chronic inflammatory diseases other than TAP deficiency (chronic lung diseases or vasculitis). Peripheral blood mononuclear cells isolated from venous blood were stained with fluorochrome-conjugated antibodies and the phenotype of NK cells was analyzed by flow cytometry. In addition, 51Chromium release assays were performed to assess the cytotoxic activity of NK cells. In the symptomatic patients, CD56bright NK cells represented 28% and 45%, respectively, of all NK cells (higher than in healthy donors). The patients also displayed a higher percentage of CD56dimCD16− NK cells than controls. Interestingly, this unusual NK cell subtype distribution was not found in the two asymptomatic TAP-deficient cases, but was instead present in several of the other patients. Over-expression of the inhibitory receptor CD94/NKG2A by TAP-deficient NK cells was confirmed and extended to the inhibitory receptor ILT2 (CD85j). These inhibitory receptors were not involved in regulating the cytotoxicity of TAP-deficient NK cells. We conclude that expansion of the CD56bright NK cell subtype in peripheral blood is not a hallmark of TAP deficiency, but can be found in other diseases as well. This might reflect a reaction of the immune system to pathologic conditions. It could be interesting to investigate the relative distribution of NK cell subsets in various respiratory and autoimmune diseases

Turning aggression into an object of intervention: Tinkering in a crime control pilot study

Real-world experiments that test new technologies can affect policy and practice by introducing new objects of intervention through tinkering; the ad hoc work of realigning relations in the face of frictions, surprises, and disturbances that occur when introducing a technology. In a pilot study on aggression detection, tinkering moved aggression in and out of the human body. In the end, the pilot defined aggression as a set of acoustic-physical variables representing the aroused human body, alongside other signals of aggression. How aggression as an object intervention was shaped by tinkering is relevant because it involved inclusions and exclusions by the authorities who identified aggression, the methods they applied, and mandate for intervention. A focus on relations that are tinkered within a real-world experiment permits critical engagement with this format. Although the real-world experimental format is credited with producing knowledge about a technology's ‘actual’ performance, actors and events at the pilot study location were made only selectively relevant. Analyses of real-world experiments should therefore explain how experiments selectively make the world relevant, giving only particular objects of intervention a truth status

Allelic differences in the relationship between proteasome activity and MHC class I peptide loading.

MHC class I molecules are cell surface glycoproteins that play a pivotal role in the response to intracellular pathogens. The loading of MHC class I molecules with antigenic substrates takes place in the endoplasmic reticulum. This requires a functional TAP transporter, which translocates peptides into the endoplasmic reticulum from the cytosol. The generation of antigenic peptides from polypeptide precursors is thought to be mediated in the cytosol by the proteasome. Previously, we have demonstrated that inhibiting the proteasome with the specific covalent inhibitor lactacystin results in a direct reduction of peptide-loaded MHC class I molecules. This indicates that the proteasome is the limiting step in the MHC class I pathway. In this study we use isoelectric focusing to demonstrate that two related MHC class I alleles, HLA-A3 and HLA-A11, as well as HLA-B35 do not follow this behavior. In contrast to other class I alleles expressed by the same cells, these alleles are loaded with peptides and mature normally when proteasome activity is severely inhibited. Our observations highlight a new level of diversity in the MHC class I system and indicate that there are allele-specific differences in the linkage between proteasome activity and MHC class I peptide loading

The major substrates for TAP in vivo are derived from newly synthesized proteins

The transporter associated with antigen processing (TAP) is a member of the family of ABC transporters that translocate a large variety of substrates across membranes. TAP transports peptides from the cytosol into the endoplasmic reticulum for binding to MHC class I molecules and for subsequent presentation to the immune system. Here we follow the lateral mobility of TAP in living cells. TAP's mobility increases when it is inactive and decreases when it translocates peptides. Because TAP activity is dependent on substrate, the mobility of TAP is used to monitor the intracellular peptide content in vivo. Comparison of the diffusion rates in peptide-free and peptide-saturated cells indicates that normally about one-third of all TAP molecules actively translocate peptides. However, during an acute influenza infection TAP becomes fully employed owing to the production and degradation of viral proteins. Furthermore, TAP activity depends on continuing protein translation. This implies that MHC class I molecules mainly sample peptides that originate from newly synthesized proteins, to ensure rapid presentation to the immune syste

Paleolatitude of the Hawaiian Hot Spot Since 48 Ma: Evidence for a Mid‐Cenozoic True Polar Stillstand Followed by Late Cenozoic True Polar Wander Coincident With Northern Hemisphere Glaciation

Paleospin axis locations since 48 Ma inferred from the distribution of equatorial sediment accumulation rates on the Pacific plate, together with paleomagnetic poles from magnetic anomaly skewness, indicate that the Hawaiian hot spot was nearly fixed in latitude from 48 to 12 Ma, but ≈3° north of its current latitude. From 48 to 12 Ma in the Pacific hot spot reference frame, which we take to be equivalent to the global hot spot reference frame, the spin axis was located near 87°N, 164°E, recording a stillstand in true polar wander. Global hot spots shifted coherently relative to the spin axis since ≈12 Ma, consistent with an episode of true polar wander, which may continue today. The motion of the spin axis away from the Hawaiian hot spot and toward Greenland since ≈12 Ma coincided with, and may have contributed to, the onset of northern hemisphere glaciation