Design of biochip microelectrode arrays for cell arrangement

Times Cited: 0 Frenea, M Lhermite, H Le Pioufle, B Fujita, H 2nd Annual International IEEE/EMBS Conference on Microtechnologies in Medicine and Biology May 02-04, 2002 Madison, wi IEEE/Engn Med & Biol Soc, Natl Inst Hlth, Defense Adv Res Project Agcy, Motorola, NimbleGen Syst Inc, PanVera Corp, Dow Chem Co, GWC Instruments, Venture Invest LLC, Univ Wisconsin-Madison, Dept Biomed Engn, IEEE/Robot & Automat Soc, PromegaInternational audienceThis paper focuses on the use of dielectrophoresis (DEP) for particle arrangement on a biochip. Our aim was to propose a device that enables the positioning of cells at regularly spaced locations using a 2D microelectrode array (prior to electropermeabilization, for instance). Whereas the use of the positive DEP force is mainly restricted to the collection of particles at electrode edges, negative DEP can be used advantageously to confine particles at existing field minima, i.e. away from the electrodes. Three different microelectrode geometries designed for negative DEP are compared in this paper. The first two configurations are composed of two interdigitated networks of triangular and rectangular shape microelectrodes respectively. In the third type of microsystem, electrode plates of opposite polarities are alternated and powered thanks to a multilayer structure. The results obtained show that a matrix arrangement of thousands of particles (such as cells, latex beads) on a large 2D microelectrode array is possible using negative DEP

Positioning living cells on a high-density electrode array by negative dielectrophoresis

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On-chip spermatozoa trapping by dielectrophoresis

International audienceThis paper deals with spermatozoa behavior under the influence of non uniform electric fields. Both bipolar and quadripolar microelectrode structures are used to study spermatozoa trapping by negative or positive dielectrophoresis (DEP). These electrode arrays have proven their efficiency to capture motile cells using the action of the dielectrophoretic force. However, fluid flow may also explain some effects observed during our experiments

On-chip spermatozoa trapping by dielectrophoresis

International audienceThis paper deals with spermatozoa behavior under the influence of non uniform electric fields. Both bipolar and quadripolar microelectrode structures are used to study spermatozoa trapping by negative or positive dielectrophoresis (DEP). These electrode arrays have proven their efficiency to capture motile cells using the action of the dielectrophoretic force. However, fluid flow may also explain some effects observed during our experiments

Micro-magnet arrays for specific single bacterial cell positioning

International audienceIn various contexts such as pathogen detection or analysis of microbial diversity where cellular heterogeneity must be taken into account, there is a growing need for tools and methods that enable microbiologists to analyze bacterial cells individually. One of the main challenges in the development of new platforms for single cell studies is to perform precise cell positioning, but the ability to specifically target cells is also important in many applications. In this work, we report the development of new strategies to selectively trap single bacterial cells upon large arrays, based on the use of micro-magnets. Escherichia coli bacteria were used to demonstrate magnetically driven bacterial cell organization. In order to provide a flexible approach adaptable to several applications in the field of microbiology, cells were magnetically and specifically labeled using two different strategies, namely immunomagnetic labeling and magnetic in situ hybridization. Results show that centimeter-sized arrays of targeted, isolated bacteria can be successfully created upon the surface of a flat magnetically patterned hard magnetic film. Efforts are now being directed towards the integration of a detection tool to provide a complete micro-system device for a variety of microbiological applications

A silicon micro-system for parallel gene transfection into arrayed cells

International audienceWe successfully made a silicon bio-micro-system able to capture several hundreds of cells as a regular array and to place each cell between microelectrodes for electroporation. We confirmed that some fluorescent dye was successfully inserted by electroporation in 75% of living captured cells; this is the first step towards parallel processing of gene insertion to individual cells