Genetic characterization of Yersinia enterocolitica collected from tonsils of slaughtered pigs

From January to March 2009, detection of pathogenic Yersinia enterocolitica was done from 900 tonsils swabs collected from 45 pig batches in one slaughterhouse. 316 Y. enterocolitica isolates were collected and confirmed as pathogenic biotype by biochemical tests. For this study, these strains were genetically characterized on the basis of their virulence genes and their PFGE profiles. Real Time PCR was used to evaluate the presence of genes ail, myfA, and ystA on the genome and the gene yadA on the pYV plasmid. PFGE analysis using XbaI enzyme was also realised

A selective chromogenic plate, YECA, for the detection of pathogenic Yersinia enterocolitica: specificity, sensibility and capacity to detect pathogenic Y. enterocolitica from pig tonsils

A new selective chromogenic plate, YECA, was tested for its specificity, sensitivity and accuracy to detect pathogenic Y. enterocolitica from pig tonsils. We tested a panel of 26 bacterial strains on YECA and compared it to PCA, CIN and YeCM media. Detection of pathogenic Y. enterocolitica was carried out on 50 pig tonsils collected in one slaughterhouse. Enrichment was done in PSB and ITC broths. Streaking on YECA and CIN was done in direct, after 24H incubation of ITC, after 48H incubation of PSB and ITC. All the plates were incubated at 30°C during 24 hours. Presence of typical colonies on CIN and YECA was checked and isolates were biotyped

Detection of Yersinia enterocolitica on slaughtered pig tonsils in France

This study was conducted to obtain data about the presence of Yersinia enterocolitica (YE) in French slaughtered pigs and to evaluate detection methods. Nine hundred tonsil swabs were taken from pigs at slaughter (one slaughterhouse in Brittany, France, 45 batches of pigs with 20 pigs sampled per batch from January to March 2009). The swabs were vortexed in 10 ml PSB broth and I ml was transferred in 9 ml lTC broth. After 48h at 25°C, PSB enrichment was streaked on CIN plates and lTC enrichment on SSDC and CIN plates

A selective chromogenic plate, YECA, for the detection of pathogenic Yersinia enterocolitica: specificity, sensibility and capacity to detect pathogenic Y. enterocolitica from pig tonsils

A new selective chromogenic plate, YECA, was tested for its specificity, sensitivity and accuracy to detect pathogenic Y. enterocolitica from pig tonsils. We tested a panel of 26 bacterial strains on YECA and compared it to PCA, CIN and YeCM media. Detection of pathogenic Y. enterocolitica was carried out on 50 pig tonsils collected in one slaughterhouse. Enrichment was done in PSB and ITC broths. Streaking on YECA and CIN was done in direct, after 24H incubation of ITC, after 48H incubation of PSB and ITC. All the plates were incubated at 30°C during 24 hours. Presence of typical colonies on CIN and YECA was checked and isolates were biotyped.</p

Genetic characterization of Yersinia enterocolitica collected from tonsils of slaughtered pigs

From January to March 2009, detection of pathogenic Yersinia enterocolitica was done from 900 tonsils swabs collected from 45 pig batches in one slaughterhouse. 316 Y. enterocolitica isolates were collected and confirmed as pathogenic biotype by biochemical tests. For this study, these strains were genetically characterized on the basis of their virulence genes and their PFGE profiles. Real Time PCR was used to evaluate the presence of genes ail, myfA, and ystA on the genome and the gene yadA on the pYV plasmid. PFGE analysis using XbaI enzyme was also realised.</p

A Selective Chromogenic Plate, YECA, for the Detection of Pathogenic Yersinia enterocolitica: Specificity, Sensitivity, and Capacity to Detect Pathogenic Y. enterocolitica from Pig Tonsils

A new selective chromogenic plate, YECA, was tested for its specificity, sensitivity, and accuracy to detect pathogenic Y. enterocolitica from pig tonsils. We tested a panel of 26 bacterial strains on YECA and compared it to PCA, CIN, and YeCM media. Detection of pathogenic Y. enterocolitica was carried out on 50 pig tonsils collected in one slaughter house. Enrichment was done in PSB and ITC broths. Streaking on YECA and CIN was done in direct, after 24H incubation of ITC, after 48H incubation of PSB and ITC. All the plates were incubated at 30∘C during 24 hours. Presence of typical colonies on CIN and YECA was checked, and isolates were biotyped. Pathogenic Y. enterocolitica strains showed an important growth on YECA with small and red fuchsia colonies while biotype 1A exhibited very few violet colonies. Enrichment in ITC during 48H gave the best performance for detecting positive samples in pathogenic Y. enterocolitica, and YECA could detect directly pathogenic Y. enterocolitica strains (2, 3, and 4). Use of YECA in combination with ITC generates a time-saver by giving a positive test in 72H

Detection of Yersinia enterocolitica on slaughtered pig tonsils in France

This study was conducted to obtain data about the presence of Yersinia enterocolitica (YE) in French slaughtered pigs and to evaluate detection methods. Nine hundred tonsil swabs were taken from pigs at slaughter (one slaughterhouse in Brittany, France, 45 batches of pigs with 20 pigs sampled per batch from January to March 2009). The swabs were vortexed in 10 ml PSB broth and I ml was transferred in 9 ml lTC broth. After 48h at 25°C, PSB enrichment was streaked on CIN plates and lTC enrichment on SSDC and CIN plates.</p