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Persistent, symptomless, systemic, and seed-borne infection of lettuce by Botrytis cinerea
Experiments are presented which show that Botrytis cinerea, the cause of gray mould disease, is often present in symptomless lettuce plants as a systemic, endophytic, infection which may arise from seed. The fungus was isolated on selective media from surface sterilized sections of roots, stem pieces and leaf discs from symptomless plants grown in a conventional glasshouse and in a spore-free air-flow provided by an isolation propagator. The presence of B. cinerea was confirmed by immuno-labelling the tissues with the Botrytis-specific monoclonal antibody BC-12.CA4. As plants grew, infection spread from the roots to stems and leaves. Surface sterilization of seeds reduced the number of infected symptomless plants. Artificial infection of seedlings with dry conidia increased the rate of infection in some experiments. Selected isolates were genetically finger-printed using microsatellite loci. This confirmed systemic spread of the inoculating isolates but showed that other isolates were also present and that single plants hosted multiple isolates. This shows that B. cinerea commonly grows in lettuce plants as an endophyte, as has already been shown for Primula. If true for other hosts, the endophytic phase may be as important a component of the species population as the aggressive necrotrophic phase
The Formation of the Double Pulsar PSR J0737-3039A/B
Recent timing observations of the double pulsar J0737-3039A/B have shown that
its transverse velocity is extremely low, only 10 km/s, and nearly in the Plane
of the Galaxy. With this new information, we rigorously re-examine the history
and formation of this system, determining estimates of the pre-supernova
companion mass, supernova kick and misalignment angle between the pre- and
post-supernova orbital planes. We find that the progenitor to the recently
formed `B' pulsar was probably less than 2 MSun, lending credence to
suggestions that this object may not have formed in a normal supernova
involving the collapse of an iron core. At the same time, the supernova kick
was likely non-zero. A comparison to the history of the double-neutron-star
binary B1534+12 suggests a range of possible parameters for the progenitors of
these systems, which should be taken into account in future binary population
syntheses and in predictions of the rate and spatial distribution of short
gamma-ray burst events.Comment: To appear in MNRAS Letters. Title typo fix only; no change to pape
Relating multi-sequence longitudinal intensity profiles and clinical covariates in new multiple sclerosis lesions
Structural magnetic resonance imaging (MRI) can be used to detect lesions in
the brains of multiple sclerosis (MS) patients. The formation of these lesions
is a complex process involving inflammation, tissue damage, and tissue repair,
all of which are visible on MRI. Here we characterize the lesion formation
process on longitudinal, multi-sequence structural MRI from 34 MS patients and
relate the longitudinal changes we observe within lesions to therapeutic
interventions. In this article, we first outline a pipeline to extract voxel
level, multi-sequence longitudinal profiles from four MRI sequences within
lesion tissue. We then propose two models to relate clinical covariates to the
longitudinal profiles. The first model is a principal component analysis (PCA)
regression model, which collapses the information from all four profiles into a
scalar value. We find that the score on the first PC identifies areas of slow,
long-term intensity changes within the lesion at a voxel level, as validated by
two experienced clinicians, a neuroradiologist and a neurologist. On a quality
scale of 1 to 4 (4 being the highest) the neuroradiologist gave the score on
the first PC a median rating of 4 (95% CI: [4,4]), and the neurologist gave it
a median rating of 3 (95% CI: [3,3]). In the PCA regression model, we find that
treatment with disease modifying therapies (p-value < 0.01), steroids (p-value
< 0.01), and being closer to the boundary of abnormal signal intensity (p-value
< 0.01) are associated with a return of a voxel to intensity values closer to
that of normal-appearing tissue. The second model is a function-on-scalar
regression, which allows for assessment of the individual time points at which
the covariates are associated with the profiles. In the function-on-scalar
regression both age and distance to the boundary were found to have a
statistically significant association with the profiles
Precision determination of absolute neutron flux
A technique for establishing the total neutron rate of a highly-collimated
monochromatic cold neutron beam was demonstrated using a method of an
alpha-gamma counter. The method involves only the counting of measured rates
and is independent of neutron cross sections, decay chain branching ratios, and
neutron beam energy. For the measurement, a target of 10B-enriched boron
carbide totally absorbed the neutrons in a monochromatic beam, and the rate of
absorbed neutrons was determined by counting 478keV gamma rays from neutron
capture on 10B with calibrated high-purity germanium detectors. A second
measurement based on Bragg diffraction from a perfect silicon crystal was
performed to determine the mean de Broglie wavelength of the beam to a
precision of 0.024 %. With these measurements, the detection efficiency of a
neutron monitor based on neutron absorption on 6Li was determined to an overall
uncertainty of 0.058 %. We discuss the principle of the alpha-gamma method and
present details of how the measurement was performed including the systematic
effects. We also describe how this method may be used for applications in
neutron dosimetry and metrology, fundamental neutron physics, and neutron cross
section measurements.Comment: 44 page
Spectral Line Imaging Observations of 1E0102.2-7219
E0102-72 is the second brightest X-ray source in the Small Magellanic Cloud
and the brightest supernova remnant in the SMC. We observed this SNR for ~140
ksec with the High Energy Transmission Gratings (HETG) aboard the Chandra X-ray
Observatory. The small angular size and high surface brightness make this an
excellent target for HETG and we resolve the remnant into individual lines. We
observe fluxes from several lines which include O VIII Ly, Ly,
and O VII along with several lines from Ne X, Ne IX and Mg XII. These line
ratios provide powerful constraints on the electron temperature and the
ionization age of the remnant.Comment: To appear in "Young Supernova Remnants" (11th Annual Astrophysics
Conference in Maryland), S. S. Holt & U. Hwang (eds), AIP, New York (2001
Introducing problem-based learning techniques into the natural resources managment curriculum at the University of Delaware
Natural Resource Management is a relatively new interdisciplinary major in the College of Agriculture and Natural Resources at the University of Delaware. This paper describes the efforts of faculty to incorporate problem-based learning techniques into several courses in the Natural Resource Management major. It includes a brief history of the major; an overview of problem-based learning programs and initiatives at the University of Delaware; a discussion of problem-based learning techniques recently introduced into courses in statistics, community economic development, environmental law, and a senior capstone course; and observations by faculty and students on the problems and benefits of problem-based learning techniques
Is there a gender difference in noninvasive coronary imaging? Multislice computed tomography for noninvasive detection of coronary stenoses
<p>Abstract</p> <p>Background</p> <p>Multislice computed tomography (MSCT) coronary angiography is the foremost alternative to invasive coronary angiography.</p> <p>Methods</p> <p>We sought to compare the diagnostic accuracy of MSCT in female and male patients with suspected coronary disease. Altogether 50 women and 95 men underwent MSCT with 0.5 mm detector collimation. Coronary artery stenoses of at least 50% on conventional coronary angiography were considered significant.</p> <p>Results</p> <p>The coronary vessel diameters of all four main coronary artery branches were significantly larger in men than in women. The diagnostic accuracy of MSCT in identifying patients with coronary artery disease was significantly lower for women (72%) compared with men (89%, <it>p </it>< 0.05). Also sensitivity (70% vs. 95%), positive predictive value (64% vs. 93%), and the rate of nondiagnostic examinations (14% vs. 4%, all: <it>p </it>< 0.05) were significantly worse for women. The effective radiation dose of MSCT coronary angiography was significantly higher in the examination of women (13.7 ± 1.2 mSv) than of men (11.7 ± 0.9 mSv, <it>p </it>< 0.001), mainly as a result of the fact that the radiosensitive female breast (contributing 24.5% of the dose in women) is in the x-ray path.</p> <p>Conclusion</p> <p>Noninvasive coronary angiography with MSCT might be less accurate and sensitive for women than men. Also, women are exposed to a significantly higher effective radiation dose than men.</p
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