Hoe cultureel is de digitale generatie?

Over the past decade, young people in the Netherlands have developed into fervent Internet users. At the same time, cultural institutions have digitised a great deal of their content and made it accessible online. This was seen as a challenging combination, or a new opportunity to reach this target audience, since most young people are online. It is however unclear how much use the digital generation actually makes of the online opportunities for cultural purposes. This report describes how culturally active teenagers are on the Internet and to what extent their cultural activities are related to the extent of their Internet use, their digital skills, the cultural activities of their family and friends, and the (cultural and digital) activities at their school. For this study, 1592 Dutch secondary school students were interviewed in 2008, with questionnaires being completed by students in 167 classes at 32 schools distributed across five large cities, 11 medium-sized cities and three small municipalities. The students varied in age from 12 to 18 years and were attending courses at pre-vocational secondary, senior general secondary or pre-university level (students in final examination classes were not interviewed). In order to investigate the digital cultural participation of 12-18 year-olds, a distinction was made between three types of Internet use. These can be summarised using three ‘C’s: − content (using the Internet to search for various kinds of information, such as text, images and sound); − contact (personal communication or interaction in peer-to-peer networks); − creation (production of text, images or sound and uploading these to the Internet, as well as responding online to contributions from others and posting tags).In het afgelopen decennium hebben jongeren zich geprofileerd als fervente internetgebruikers. Tegelijkertijd hebben culturele instellingen veel aanbod gedigitaliseerd en online toegankelijk gemaakt. Dit werd als een uitdagende combinatie of een nieuwe kans gezien, aangezien de meeste jongeren online zijn. Het is echter de vraag in hoeverre de digitale generatie de online mogelijkheden inderdaad benut voor culturele doeleinden. In dit rapport is beschreven hoe cultureel actief tieners op internet zijn en in hoeverre die activiteiten samenhangen met de omvang van hun internetgebruik, hun digitale vaardigheden, de culturele activiteiten in hun gezin en van hun vrienden, en de (culturele en digitale) activiteiten op hun school. Voor het onderzoek zijn leerlingen in het voortgezet onderwijs geïnterviewd. In 2008 vulden 1592 leerlingen uit 167 klassen van 32 scholen in de klas een vragenlijst in. De scholen zijn verdeeld over vijf grote steden, elf middelgrote steden en drie kleine gemeenten. De leerlingen varieerden in leeftijd tussen de 12 en 18 jaar en zaten op een vmbo-, havo- of vwo-opleiding (leerlingen in eindexamenklassen zijn niet ondervraagd). Om de digitale cultuurparticipatie van 12-18-jarige scholieren te onderzoeken is een onderscheid gemaakt in drie rollen bij het internetgebruik. Deze drie rollen kunnen samengevat worden met drie C’s: − content (gebruik internet voor verschillende soorten informatie zoals tekst, beeld als geluid); − contact (persoonlijke communicatie of interactie in peer-to-peer-netwerken); − creatie (het zelf produceren van tekst, beeld of geluid en deze uploaden op internetalsmede het online reageren op bijdragen van anderen en het plaatsen van tags)

Knowledge of pressure ulcer prevention: a cross-sectional and comparative study among nurses

BACKGROUND: Pressure ulcers are a common, painful and costly condition. Results of a 1991 study into the knowledge among Dutch hospital nurses on the usefulness of measures to prevent pressure ulcers showed moderate knowledge. Results were confirmed by subsequent studies. In recent years, Dutch guidelines have been updated and the attention given to pressure ulcer care has been increased. This was expected to improve pressure ulcer care and to increase nurses' knowledge. The aims of the current study were to investigate (1) how much nurses employed in Dutch hospitals know about the usefulness of 28 preventive measures considered in the most recent national pressure ulcer guideline; (2) whether differences in knowledge exist between nurses working in hospitals that audit pressure ulcers and those employed in hospitals that do not; and (3) to study whether knowledge among Dutch hospital nurses regarding the usefulness of preventive measures had changed between 1991 and 2003. METHODS: A cross-sectional study design among nurses employed in Dutch hospitals in 2003 was used to investigate their knowledge and differences in knowledge between nurses employed in different types of institution. A comparative design was used to assess whether knowledge differed between this population and that of Dutch hospital nurses in 1991. The nurses' knowledge was assessed by a written questionnaire. Data of 522 respondents meeting the inclusion criteria were analyzed and compared with the results of the 351 nurses included in the 1991 study. RESULTS: Knowledge in 2003 was slightly better than that in 1991. The nurses were moderately aware of the usefulness of preventive measures. Nurses employed in organizations that monitored pressure ulcers did not display greater knowledge than those employed in organizations that did not do so. CONCLUSION: Knowledge among Dutch hospital nurses about the usefulness of measures to prevent pressure ulcers seems to be moderate. Being employed in an institution that monitors pressure ulcer care hardly affects the knowledge level. Knowledge about prevention has improved little since 1991

The lipid droplet coat protein perilipin 5 also localizes to muscle mitochondria

Perilipin 5 (PLIN5/OXPAT) is a lipid droplet (LD) coat protein mainly present in tissues with a high fat-oxidative capacity, suggesting a role for PLIN5 in facilitating fatty acid oxidation. Here, we investigated the role of PLIN5 in fat oxidation in skeletal muscle. In human skeletal muscle, we observed that PLIN5 (but not PLIN2) protein content correlated tightly with OXPHOS content and in rat muscle PLIN5 content correlated with mitochondrial respiration rates on a lipid-derived substrate. This prompted us to examine PLIN5 protein expression in skeletal muscle mitochondria by means of immunogold electron microscopy and Western blots in isolated mitochondria. These data show that PLIN5, in contrast to PLIN2, not only localizes to LD but also to mitochondria, possibly facilitating fatty acid oxidation. Unilateral overexpression of PLIN5 in rat anterior tibialis muscle augmented myocellular fat storage without increasing mitochondrial density as indicated by the lack of change in protein content of five components of the OXPHOS system. Mitochondria isolated from PLIN5 overexpressing muscles did not possess increased fatty acid respiration. Interestingly though, 14C-palmitate oxidation assays in muscle homogenates from PLIN5 overexpressing muscles revealed a 44.8% (P = 0.05) increase in complete fatty acid oxidation. Thus, in mitochondrial isolations devoid of LD, PLIN5 does not augment fat oxidation, while in homogenates containing PLIN5-coated LD, fat oxidation is higher upon PLIN5 overexpression. The presence of PLIN5 in mitochondria helps to understand why PLIN5, in contrast to PLIN2, is of specific importance in fat oxidative tissues. Our data suggests involvement of PLIN5 in directing fatty acids from the LD to mitochondrial fatty acid oxidation

Assessing Causality in the Relationship Between Adolescents’ Risky Sexual Online Behavior and Their Perceptions of this Behavior

The main aim of this study was to investigate the causal nature of the relationship between adolescents’ risky sexual behavior on the internet and their perceptions of this behavior. Engagement in the following online behaviors was assessed: searching online for someone to talk about sex, searching online for someone to have sex, sending intimate photos or videos to someone online, and sending one’s telephone number and address to someone exclusively known online. The relationship between these behaviors and adolescents’ perceptions of peer involvement, personal invulnerability, and risks and benefits was investigated. A two-wave longitudinal study among a representative sample of 1,445 Dutch adolescents aged 12–17 was conducted (49% females). Autoregressive cross-lagged structural equation models revealed that perceived peer involvement, perceived vulnerability, and perceived risks were all significant predictors of risky sexual online behavior 6 months later. No reverse causal paths were found. When the relationships between perceptions and risky sexual online behavior were modeled simultaneously, only perceived peer involvement was a determinant of risky sexual online behavior. Findings highlight the importance of addressing peer involvement in future interventions to reduce adolescents’ risky sexual online behavior

An organoid-derived bronchioalveolar model for SARS-CoV-2 infection of human alveolar type II-like cells

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes coronavirus disease 2019 (COVID-19), which may result in acute respiratory distress syndrome (ARDS), multiorg