Within-host competition between two entomopathogenic fungi and a granulovirus in Diatraea saccharalis (Lepidoptera: Crambidae).

Abstract: We provide insights into how the interactions of two entomopathogenic fungi and a virus play a role in virulence, disease development, and pathogen reproduction for an economically important insect crop pest, the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae). In our model system, we highlight the antagonistic effects of the co-inoculation of Beauveria bassiana and granulovirus (DisaGV) on virulence, compared to their single counterparts. By contrast, combinations of Metarhizium anisopliae and B. bassiana, or M. anisopliae and DisaGV, have resulted in additive effects against the insect. Intriguingly, most cadavers that were derived from dual or triple infections, produced signs/symptoms of only one species after the death of the infected host. In the combination of fungi and DisaGV, there was a trend where a higher proportion of viral infection bearing conspicuous symptoms occurred, except when the larvae were inoculated with M. anisopliae and DisaGV at the two highest inoculum rates. Co-infections with B. bassiana and M. anisopliae did not affect pathogen reproduction, since the sporulation from co-inoculated larvae did not differ from their single counterparts

Insecticidal Activity of the Granulosis Virus in Combination with Neem Products and Talc Powder Against the Potato Tuberworm Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)

The potato tuberworm Phthorimaea operculella (Zeller) is an important agricultural pest that causes significant economic losses to potato growers worldwide. The addition of an effective method of biological control for the potato tuberworm is greatly needed, and is currently unavailable in Brazil. The granulosis virus (Baculoviridae) is a promising biological control agent to protect post-harvest potatoes and in storage from the potato tuberworm. However, the control measure must be economically feasible. Liquid suspensions of a granulosis virus applied alone or in mixture with two commercial neem oil-based products (DalNeem (TM) and NeemAzal (TM)), and a dry powder formulation of viral granules were evaluated for control of potato tuberworm larvae by treating potato tubers under laboratory conditions. High larval mortality (86.7%) was achieved when DalNeem and virus were applied together at 4 mg of azadirachtin/L and 10(4) occlusion bodies (OBs)/mL, respectively. This combination resulted in a parts per thousand yen50% efficacy in relation to their counterparts alone. Conversely, NeemAzal did not enhance virus effectiveness against larvae of the potato tuberworm. The talc-based virus formulation was used for dusting seed tubers at different concentrations and resulted in 100% larval mortality at 5 x 10(8) OBs/g. Formulated and unformulated virus provided 50% mortality at 166 OBs/g and at 5.0 x 10(5) OBs/mL, respectively. As a result, talc-based virus formulation had a better control efficiency on potato tuberworm than the aqueous virus suspension. The granulosis virus combined with DalNeem at low rates or formulated with talc powder is a viable option to control the potato tuberworm under storage conditions.CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES

Virulência de fungos entomopatogênicos a ninfas de Bemisia tabaci biótipo B (Hemiptera: Aleyrodidae).

Neste trabalho, avaliou-se a virulência de 14 isolados dos fungos Beauveria bassiana senso latu, Isaria fumosorosea e Lecanicillium spp. sobre ninfas de segundo ínstar de Bemisia tabaci biótipo B em folhas de feijoeiro (cv. Pérola) sob condições de laboratório (26°C, UR>80%, 14 h luz) e, em seguida, determinou-se a capacidade desses isolados em produzir inóculo sobre cadáveres do inseto

Transcriptional responses of Beauveria bassiana blastospores cultured under varying glucose concentrations.

Abstract: Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG signaling pathway with upregulation of homologous genes Ssk2 and Hog1 for all fermentation time points under hyperosmotic medium (20% glucose). These findings expand our knowledge of the molecular mechanisms behind blastospore development and may help facilitate large-scale industrial production of B. bassiana blastospores for pest control applications

Seletividade de agroquimicos a Mononychellus tanajoa (Bondar) e Amblyseius idaeus (Denmark & Muma) (Acari: Tetranychidae e Phytoseiidae).

Mononychellus tanajoa e uma das principais pragas da mandioca. Devido as caracteristicas da cultura e da praga tem sido proposto o uso de inimigos naturais para seu controle. Dentre estes, A. idaeus e um dos predadores de M. tanajoa encontrado com maior frequencia no nordeste brasileiro. Para avaliacao de sua eficiencia, usa-se comparar a flutuacao da praga entre parcelas onde o predador esteja presente e, onde o predador seja excluido mediante a utilizacao de produtos de seletividade inversa. Com o objetivo de se conhecer os produtos mais indicados para este estudo, foram realizados ensaios em laboratorio. O metodo de imersao de laminas foi utilizado para essas duas especies de acaros sendo realizadas quatro repeticoes a cinco concentracoes diferentes para cada produto. Foram utilizados: abamectina, carbaril, deltametrina, dimetoato, dicofol, endosulfan, mation, paration metilico, permetrina e trazofos. Deltrametrina, dimetoato, permetrina e triazofos mostraram-se promissores para utilizacao a nivel de campo, por serem mais toxicos a A. idaeus e menos toxicos a M. tanajoa

Ácaros predadores e o fungo Neozygites floridana associados a tetraniquídeos em soja no Rio Grande do Sul.

O objetivo deste trabalho foi verificar a ocorrência de ácaros predadores e do fungo Neozygites floridana, associados a ácaros tetraniquídeos, em soja (Glycine max L.), no Estado do Rio Grande do Sul. Entre 2003 e 2005, foram avaliadas amostras de folhas de soja das principais regiões produtoras do Estado. Essas amostras foram coletadas de diferentes posições da planta e aleatoriamente na lavoura. As espécies de ácaros predadores encontradas foram: Neoseiulus anonymus, N. californicus, Phytoseiulus fragariae, P. macropilis, Proprioseiopsis cannaensis e Galendromus annectens. O fungo N. floridana apresentou ampla distribuição e ocorreu em todas as espécies de tetraniquídeos encontradas: Mononychellus planki, Tetranychus desertorum, T. gigas, T. ludeni e T. urticae

Adequação de propriedades rurais ao Código Florestal Brasileiro: estudo de caso no Estado do Paraná.

O Código Florestal Brasileiro de 1965 conceitua e regulamenta as Áreas de Preservação Permanente (APP) e Reserva Legal (RL). Para regulamentar a adequação das propriedades agrícolas ao código florestal, o estado do Paraná instituiu o SISLEG - Sistema de Manutenção, Recuperação e Proteção da Reserva Legal e Áreas de Preservação Permanente. Neste estudo foram quantificadas, com auxílio do sistema de posicionamento global (GPS) e programas de Sistemas de Informações Geográficas (SIG), as APPs e RLs de 147 propriedades em oito municípios das regiões centro-oriental e sudeste do Paraná, visando analisá-las frente ao Código Florestal vigente. Observou-se expressiva cobertura florestal formada por fragmentos de Floresta Ombrófila Mista; as superfícies disponíveis para definição de RL apresentam valores superiores àqueles requeridos pela legislação. Há necessidade de recomposição das APPs em 67% das propriedades; 26% das propriedades estão de acordo com as exigências do Código Florestal. A quantificação da cobertura florestal necessária e remanescente, tal como a pequena demanda de área para readequação de APP (5% da área total) permitem inferir que esses quesitos não são entraves para a regularização ambiental da agricultura familiar, nas propriedades analisadas