NBN: IT The Italian trusted persistent identifier infrastructure

This paper describes how the Italian national bibliography number (NBN) service has become a trusted infrastructure when it has been combined with digital stacks, the national digital preservation infrastructure. A suitable descriptive model, the bricks of trust, to assess the trustworthiness of the PI systems, has been provided. The Italian NBN has been positively assessed according to this model

Interoperability knowledge base for persistent identifiers interoperability framework

In this paper, we propose a semantic web based solution to implement the Interoperability Framework (IF) for Persistent Identifiers (PI) developed within the context of APARSEN EU project. The IF provides a comprehensive, semantics-aware solution for interoperability of heterogeneous Persistent Identifier systems. Such a solution aims to provide added-value services built on an Interoperability Knowledge Base. The IF ontology refinement and the related prototype specifics have been designed adopting a bottom-up approach that starts from a) the analysis of metadata provided by Content Providers and b) the collection of functional and semantic requirements of Persistent Identifier Domains (PID to fostering ontology-based metadata translation among different bodies. Conclusions and intended future work close the paper

BCR-ABL prevents c-Jun-mediated and proteasome-dependent FUS (TLS) proteolysis through a protein kinase C beta II-dependent pathway

The DNA binding activity of FUS (also known as TLS), a nuclear pro-oncogene involved in multiple translocations, is regulated by BCR-ABL in a protein kinase cpn (PKC beta II)-dependent manner. We show here that in normal myeloid progenitor cells FUS, although not visibly ubiquitinated, undergoes proteasome-dependent degradation, whereas in BCR-ABL-expressing cells, degradation is suppressed by PKC beta II phosphorylation. Replacement of serine 256 with the phosphomimetic aspartic acid prevents proteasome-dependent proteolysis of FUS, while the serine-256-to-alanine FUS mutant is unstable and susceptible to degradation. Ectopic expression of the phosphomimetic S256D FUS mutant in granulocyte colony-stimulating factor-treated 32Dcl3 cells induces massive apoptosis and inhibits the differentiation of the cells escaping cell death, while the degradation-prone S256A mutant has no effect on either survival or differentiation. FUS proteolysis is induced by c-Jun, is suppressed by BCR-ABL or Jun kinase 1, and does not depend on c-Jun transactivation potential, ubiquitination, or its interaction with Jun kinase 1, In addition, c-Jun induced FUS proteasome-dependent degradation is enhanced by heterogeneous nuclear ribonucleoprotein (hnRNP) A1 and depends on the formation of a FUS-Jun-hnRNP A1-containing complex and on lack of PKC beta II phosphorylation at serine 256 but not on FUS ubiquitination. Thus, novel mechanisms appear to be involved in the degradation of FUS in normal myeloid cells; moreover, the ability of the BCR-ABL oncoprotein to suppress FUS degradation by the induction of posttranslational modifications might contribute to the phenotype of BCR-ABL expressing hematopoietic cells

Tradition and modernity in the mediterranean cities. The tunisian cities: Tunis, Kairouan, Tozeur, Nefta.

L’articolo fa un confronto tra le differenti forme urbane delle città del Nord Africa, in particolare quelle della Tunisia (Tunisi, Kairouan, Tozeur, Nefta). Tali città, distribuite tra il Nord ed il Sud del paese, esprimono, nelle loro medine, l’identità della forma urbana e ne dichiarano la modernità lungo i loro bordi. Tunisi, posta tra due laghi, costruisce la sua modernità con la città coloniale che, anche se con linguaggi diversi, evidenzia i temi del Mediterraneo. La città di Kairouan mantiene la sua identità nella grande moschea, luogo centrale di organizzazione urbana. Le città d’oasi, come Tozeur e Nefta, esprimono il loro rapporto col paesaggio ed evidenziano con le loro architetture i materiali usati