Acute myeloid leukemia of donor cell origin after allogeneic stem cell transplantation in a patient with acute myeloid leukemia

41st Annual Meeting of the European-Society-for-Blood-and-Marrow-Transplantation -- MAR 22-25, 2015 -- Istanbul, TURKEYWOS: 000351632903216…European Soc Blood & Marrow Transplanta

Non-invasive prenatal diagnosis of fetal RhD by using free fetal DNA

WOS: 000356108200017PubMed ID: 26152007Objective: Anti-D immunoglobulin is applied to all pregnant women having Rh]) incompatibility to prevent hemolytic disease of the newborn. The aim of this study is to determine fetal RE]) status in the Rh incompatible pregnancies with an non-invasive technique; free fetal DNA isolation from maternal circulation. In the case of Rh incompatibility especially with a history of previous fetal anemia, it can be beneficial to know Rh status antenatally in terms of monitoring fetuses with Rh positive [RhD(+)] status consciously. Materials and Methods: Total free DNA was isolated in 50 Rh negative [RhD()] pregnant women, who had Rh]) alloimmunisation with their husbands. The gene in isolated DNA was investigated with TagMan prob and real time PCR by using primers belonging to exon 7 of the RhD gene. Results: The authors analyzed 50 RhD() women by using quantitative real time PCR technique. Five of them were RhD() and the rest of them were found to be RhD(+). After birth one of the infants who were analyzed as RhD(+) were found to be RED(). Conclusion: The detection of fetal RED status by using a non-invasive method from maternal circulation was found to be possible. Assessing fetal RED status non-invasively by using free fetal DNA in maternal blood will be cost-efficient, avoiding unnecessary indirect Coombs test and unnecessary Rhogam applications that is used in RH incompatible pregnancies. This study will throw a fresh light on prenatal diagnosis

Genetic Variations in the Xenobiotic-Metabolizing Enzymes CYP1A1

Cytochrome P450 (CYP) enzymes are genetically polymorphic and play key roles in the metabolism of xenobiotics. Colorectal cancer (CRC) is one of the most common malignant tumors in Turkey as well as in the world. In this study, it was aimed both to evaluate the effects of CYP variants on the susceptibility to CRC and to predict the individual response of the Turkish people to xenobiotics metabolized by CYP enzymes. For that, we assessed the association of CYP1A1, CYP1A2, CYP2C9, and CYP2C19 polymorphisms in patients with CRC in the Turkish population through a case-control study. Distributions of the variants were determined in 104 patients with CRC and 183 healthy volunteers. As results, CYP1A1 6235T/C was significantly associated with CRC risk (odds ratio [OR]=2.53; 95% confidence interval [CI]=0.99-6.45; p=0.046). In a haplotype-based analysis, CYP1A1 haplotype C-6235-A(2455) might be associated with the development of CRC (OR=2.70; 95% CI=0.58-5.90; p=0.046). We believe that the findings are the first results of CYP allele distributions in the Turkish population and provide an understanding of the epidemiological studies that correlate therapeutic approaches and etiology of CRC especially in Turkish patients