Gold- and silver-based nano-particles influence pseudo-typed lenti-viral infection

The application of bio-active noble-metal-based nano-particles (NPs) with unique physico-chemical properties is multifaceted. Among other roles, they can be used as anti-viral agents, and at the same time might serve as matrix to facilitate the transport of various molecules for therapeutic purposes directly or via genetically modified microbes. For this reason, the influence of nano-materials on viral infection in living cells is described in this study utilizing pseudo-typed lenti-viral particles based on human immuno-deficiency virus 1 (HIV-1). Cells were exposed to various NPs and subsequently infected with lenti-virus. Transfection efficiency was quantified by flow-cytometry analysis. Gold-based NPs increased, silver-containing NPs decreased, while other NPs had little or no effect on viral infection rate. The opposing effect of NPs is determined by the size, chemical nature and surface chemistry of the nano-materials, which govern their interactions with molecular species present in their environment. These characteristics enable the distinct use of different NPs in various fields of bio-medicine. © 2013 Bentham Science Publishers

Gold- and silver-based nano-particles influence pseudo-typed lenti-viral infection

The application of bio-active noble-metal-based nano-particles (NPs) with unique physico-chemical properties is multifaceted. Among other roles, they can be used as anti-viral agents, and at the same time might serve as matrix to facilitate the transport of various molecules for therapeutic purposes directly or via genetically modified microbes. For this reason, the influence of nano-materials on viral infection in living cells is described in this study utilizing pseudo-typed lenti-viral particles based on human immuno-deficiency virus 1 (HIV-1). Cells were exposed to various NPs and subsequently infected with lenti-virus. Transfection efficiency was quantified by flow-cytometry analysis. Gold-based NPs increased, silver-containing NPs decreased, while other NPs had little or no effect on viral infection rate. The opposing effect of NPs is determined by the size, chemical nature and surface chemistry of the nano-materials, which govern their interactions with molecular species present in their environment. These characteristics enable the distinct use of different NPs in various fields of bio-medicine. © 2013 Bentham Science Publishers

Gold- and silver-based nano-particles influence pseudo-typed lenti-viral infection

The application of bio-active noble-metal-based nano-particles (NPs) with unique physico-chemical properties is multifaceted. Among other roles, they can be used as anti-viral agents, and at the same time might serve as matrix to facilitate the transport of various molecules for therapeutic purposes directly or via genetically modified microbes. For this reason, the influence of nano-materials on viral infection in living cells is described in this study utilizing pseudo-typed lenti-viral particles based on human immuno-deficiency virus 1 (HIV-1). Cells were exposed to various NPs and subsequently infected with lenti-virus. Transfection efficiency was quantified by flow-cytometry analysis. Gold-based NPs increased, silver-containing NPs decreased, while other NPs had little or no effect on viral infection rate. The opposing effect of NPs is determined by the size, chemical nature and surface chemistry of the nano-materials, which govern their interactions with molecular species present in their environment. These characteristics enable the distinct use of different NPs in various fields of bio-medicine. © 2013 Bentham Science Publishers

Gold- and silver-based nano-particles influence pseudo-typed lenti-viral infection

The application of bio-active noble-metal-based nano-particles (NPs) with unique physico-chemical properties is multifaceted. Among other roles, they can be used as anti-viral agents, and at the same time might serve as matrix to facilitate the transport of various molecules for therapeutic purposes directly or via genetically modified microbes. For this reason, the influence of nano-materials on viral infection in living cells is described in this study utilizing pseudo-typed lenti-viral particles based on human immuno-deficiency virus 1 (HIV-1). Cells were exposed to various NPs and subsequently infected with lenti-virus. Transfection efficiency was quantified by flow-cytometry analysis. Gold-based NPs increased, silver-containing NPs decreased, while other NPs had little or no effect on viral infection rate. The opposing effect of NPs is determined by the size, chemical nature and surface chemistry of the nano-materials, which govern their interactions with molecular species present in their environment. These characteristics enable the distinct use of different NPs in various fields of bio-medicine. © 2013 Bentham Science Publishers

3D Bioprinting of aortic structures with HDF cells

Traditionally, tissue engineering strategies for damaged or diseased large blood vessels are based on scaffolds. Following the cell-seeding and surgical implantation of scaffold, there could be immunogenic reactions and complications due to the limitations of scaffold biomaterials. Therefore, recent vascular tissue engineering studies tends to scaffold-free techniques [1, 2]. In this research, a novel biomimetic computer-aided design and three-dimensional (3D) bioprinting techniques are developed to optimize bioprinting of scaffold-free macrovascular structures biomimicking a real human aorta. Live human dermal fibroblast cells (HDF) aggregates and support structures (hydrogels) are 3D bioprinted layer-by-layer according to the developed self-supporting computer model to form biomimetic aortic vessel structures

3D Bioprinting of branched vessel constructs

A novel scaffold-free method for 3D bioprinting complex branched blood vessels is presented. Branched vascular structures gained importance due to the challenges of scaffold vascularization. In literature, there are several approaches to construct multicellular networks which have limited functionalities [1, 2].Traditional tissue engineering studies depend on scaffolds. However, cell seeding into scaffolds and in-vivo implantation of scaffolds could have immunogenic and unaccepted side effects. Therefore, computer-aided models of the branched vascular structures are developed allowing printing both the support structures and living cells layer-by-layer. Methodology is implemented in Rhino 3D software using Rhinoscript language. The methods are utilized to 3D bioprint branched vascular structures directly from generated Zig-zag computer models