Evaluating the In vivo Efficacy of Copper-Chitosan Nanocomposition for Treating Vascular Wilt Disease in Date Palm

Date palm, Phoenix dactylifera, as one of the most important fruit crops in Egypt and many other countries, can be affected by many fungal diseases, among which the vascular wilt disease, caused by the fungal pathogen Fusarium oxysporum, is considered the most deteriorating one. This study aims at evaluating the efficiency of Copper-Chitosan Nanopcomposition for treating the vascular wilt disease in date palm. The study relies mainly on beleaguering the disease via the double-role functionality of copper-chitosan nanocomposition, i.e. its potential antifungal effect on the fungal pathogen, besides its capability to enhance the immune responses of the infected plant. In this regard, chitosan nanoparticles were prepared according to the ionic gelation method, whereas copper nanoparticles were prepared according to the chemical reduction method. Physicochemical characterization of both chitosan and copper nanoparticles was performed using dynamic light scattering (DLS), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR) and x-ray diffraction (XRD). Copper-chitosan nanocomposition could significantly reduce the vascular wilt disease severity; this means that the nanocomposition can be used in the future for developing new nano-fungicides to control such pathogens

Can deficit irrigations be an optimum solution for increasing water productivity under arid conditions? A case study on wheat plants

Water scarcity is of growing concern in many countries around the world, especially within the arid and semi-arid zones. Accordingly, rationalizing irrigation water has become an obligation to achieve the sustainable developmental goals of these countries. This may take place via using deficit irrigation which is long thought to be an effective strategy to save and improve water productivity. The current study is a trial to evaluate the pros and cons of using 50 and 75 % of the irrigation requirements (IR) of wheat (deficit irrigations) versus 100 %IR, while precisely charting changes in wheat growth parameters, antioxidant enzymes in plant shoots and the overall nutritional status of plants (NPK contents). Accordingly, a field experiment was conducted for two successive seasons, followed a split-plot design in which deficit irrigations (two irrigations to achieve 50 % of the irrigations requirements (IR), three irrigations to attain 75 % IR, and four irrigations to fulfill 100 % IR) were placed in main plots while four different studied wheat cultivars were in subplots. Results obtained herein indicate that deficit irrigations led to significant reductions in growth parameters and productivity of all wheat cultivars, especially when using 50 % IR. It also decreased NPK contents within plant shoots while elevated their contents of proline, peroxidase, and catalase enzymes. On the other hand, this type of irrigation decreased virtual water content (VWC, the amount of water used in production on ton of wheat grains). Stress tolerance index (STI), and financial revenues per unit area were also assessed. The obtained values of grain productivity, STI, VWC and financial revenues were weighted via PCA analyses, and then introduced in a novel model to estimate the efficiency of deficit irrigations (ODEI) whose results specified that the overall efficiency decreased as follows: 50 %IR < 75 %IR < 100 %IR. In conclusion, deficit irrigation is not deemed appropriate for rationalizing irrigation water while growing wheat on arid soils

Influence of preharvest spraying with the chemical salts on grey mold disease and keeping quality of table grapes during storage

The efficacy of calcium chloride, calcium nitrate, potassium metabisulfate, calcium carbonate and sodium bicarbonate to protect grape bunches during harvesting was studied under storage conditions. This experiment was carried out in a private vineyard, El-Khatatba region, Alexandria desert road, Egypt during 2014 and 2015 growing seasons on Thompson seedless variety. The chemical used at 0.1 and 0.2% g /liter water. The healthy grape bunches of all treatments were harvested at proper ripeness, packed in perforated plastic bags, put inside carton boxes and stored at 0oC under 90-95% R.H. for 36 days. Other grape bunches either treated or untreated with the salts were artificially inoculated with spore suspension of Botrytis cinerea at a concentration of 3x106 spores/ml before packing and cold storage. Disease severity of grape bunches with B. cinerea was estimated after 36 days of cold storage. All treatments exhibited disease reduction under field conditions of Thompson seedless grape variety. The field applications of salts gave a significant reduction of botrytis rot during storage. In this respect, the grape rots were significantly reduced when sprayed by the salts three times

Adverse effects of energy drink on rat pancreas and the therapeutic role of each of bone marrow mesenchymal stem cells and Nigella Sativa oil

Background: Energy drinks have been observed to threaten public health leading to many medical problems. Bone marrow-derived mesenchymal stem cells (BMSCs) have broad prospects in tissue regeneration. Nigella Sativa (NS) possess great therapeutic properties for the treatment of a wide range of diseases. Materials and methods: Forty adult male albino rats were divided into: control group and treated group. The treated group was further subdivided into: energy drink subgroup 2a, BMSCs-injected subgroup 2b, NS-injected subgroup 2c. Histological, immunohistochemical and biochemical assessment was performed. Results: Administration of energy drink revealed that it adversely affected the pancreatic cytoarchitecture. BMSCs and NS have been similarly observed to significantly ameliorate the histological, biochemical and immunohistochemical changes induced by energy drink. Conclusions: The extent of pancreatic regeneration, exerted by each of BMSCs and NS oil, is nearly similar but the effect of BMSCs is more superior; however, NS could be privileged to BMSCs as a line of treatment being easily accessible and of lower cost

Screening of some sorghum genotypes for resistance to sorghum midge, Stenodiplosis (= Contarinia) sorghicola Coqillet (diptera: Cecidomyiidae) under gedarif rainfed conditions

Preliminary experiments were conducted at Northern area, Gedarif State during seasons of 2002-03, 2006-07.Where as advanced trials during 2008/2009 to 2012/2013 at northern and Southern areas. The objective was to evaluate selected sorghum genotypes for sorghum midge resistance. A total of 3000 accessions were obtained from Gene Bank Resources. Resistant genotype, DJ 6514 (Resistant Check already released in 2007) was obtained from International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). Result showed that the midge damage rating was significantly different among genotypes. The midge damage rating scores ranged between, 1.3- 8.6; 1.1- 9.2; 1.4- 9.0; 1.1 – 9.4; and 1.2- 9.2 for all seasons (2008/09; 2009/10; 2010/11; 2011/12 and 2012/13). However, the lowest midge damage rating was recorded by DJ 6514 (Resistant check), followed by P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Wad Baco; and Safra (1.4; 1.5; 1.5, 1.6 and 1.7), respectively. Genotypes, P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Wad Baco; Safra and Harerai showed lowest % yield loss and performed similar to the resistant check (14.2; 14.5; 14.5; 17.0 and 17.3%), respectively. The combined analysis showed that a significant difference was observed between genotypes. The genotypes were significantly different in panicle types, compact and semi-compact headed genotypes showed lower % glumes coverage (1.3- 4.5%), while semi-compact headed genotypes ranged between 5.5 – 7.8%. Genotypes, Wad Baco; P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Safra; Wad Ahmed; Harerai and Wad Akar showed the shortest glumes coverage (1.3; 1.3; 1.5; 1.5; 1.6; 1.7 and 1.8 %). Compact headed genotypes recorded lowest midge density (6.5 adults/ 5 heads), while the semi-compact headed genotypes were recorded 15.7 adults/ 5 heads compared with others types of heads