New tool for biological dosimetry: Reevaluation and automation of the gold standard method following telomere and centromere staining.

International audienceThe dicentric chromosome (dicentric) assay is the international gold-standard method for bio-logical dosimetry and classification of genotoxic agents. The introduction of telomere and centromere(TC) staining offers the potential to render dicentric scoring more efficient and robust. In this study,we improved the detection of dicentrics and all unstable chromosomal aberrations (CA) leading to asignificant reevaluation of the dose–effect curve and developed an automated approach following TCstaining